General Cell Based Assays
![Thermo Scientific™ LanthaScreen™ LRRK2 G2019S [pSer935] Cellular Assay Kit](https://assets.fishersci.com/TFS-Assets/LSG/product-images/A14686_LRRK2_G2019S_Kit_W.jpg-250.jpg)
Thermo Scientific™ LanthaScreen™ LRRK2 G2019S [pSer935] Cellular Assay Kit
The LanthaScreen™ LRRK2 G2019S [pSer935] Cellular Assay Kit combines the flexibility of the BacMam gene delivery system with the robustness and power of our LanthaScreen™ TR-FRET technology.
![Thermo Scientific™ BacMam Histone H3 [AcLys9] Cellular Assay Kit](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-250.jpg)
Thermo Scientific™ BacMam Histone H3 [AcLys9] Cellular Assay Kit
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of histones.
Thermo Scientific™ BacMam Akt [pThr308] Cellular Assay Kit
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT.
Thermo Scientific™ BacMam Akt [pSer473] Cellular Assay Kit
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT.
Cayman Chemical MitoCheck Complex IV Activity Assay Kit, 96 wells
Complex IV (cytochrome c oxidase) is the terminal electron acceptor in the mitochondrial electron transport chain. Complex IV functions by oxidizing cytochrome c and completing a four-electron reduction of oxygen (O2) to form water. During this process, two H+ are translocated from the mitochondrial matrix to the intermembrane space, contributing the mitochondrial membrane potential required for ATP synthesis. Common inhibitors of complex IV include potassium cyanide, azide, O2 limitation, nitric oxide, and carbon monoxide. Caymans MitoCheck Complex IV Activity Assay is designed to measure the direct oxidation of cytochrome c by complex IV, in an isolated bovine heart mitochondrial system that is supplied within the kit.
Cayman Chemical Glutathione Peroxidase Assay Kit, 480 wells
GPX catalyzes the reduction of hydroperoxides, including H2O2, using reduced glutathione and thereby functions to protect the cell from oxidative damage. Cayman’s Glutathione Peroxidase Assay measures GPX activity indirectly by a coupled reaction with GR. Oxidized GSSG, produced upon reduction of an organic hydroperoxide by GPX, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the GPX activity in the sample. Cayman’s GPX Assay can be used to measure all of the glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.
Eurofins Discoverx PathHunter C5AR1 Bioassay Kit, 10-Plate. (93-0557Y2-00060)
The PathHunter C5AR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cayman Chemical OXYGEN CONSUMPTION RATE ASSAY
For the measurement of oxygen consumption rate in living cells