General Cell Based Assays
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES METHANOL QUANT ASSAY KIT
NC3652522 METHANOL QUANT ASSAY KIT
Cayman Chemical SphngosIn KInase 2 InhIbIto 96
A DAPK1 (IC50 200 nM) and DAPK3 inhibitor selectively binds to DAPK1 DAPK2 and DAPK3 (Kds 300 79 and 280 nM respectively) over Pim-1 Pim-2 and Pim-3 (Kds 1800 6500 and 810 nM respectively) but does inhibit Pim-3 (IC50 200 nM) inhibits phosphorylation of RLC20 in primary human coronary artery smooth muscle cells at 10 UM reduces phenylephrine-induced contractions in isolated mouse aortic rings and increases the time to reach 50 maximal contractions in calyculin A-stimulated isolated rat caudal arterial smooth muscle strips at 10 and 100 UM respectively
Abcam Calcium Assay Kit (Colorimetric) (ab102505) 250 tests
Assay type: QuantitativePlatform: Microplate readerAssay time: 20 minSample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Cayman Chemical L-LACTATE ASSAY KIT
A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva
Cayman Chemical Glycogen Assay Kit, 96 wells
Glycogen is the principal storage form of glucose in animal and human cells. It is made primarily by the liver and muscles, but it can also be made by glycogenesis within the brain and stomach. Cayman’s Glycogen Assay provides a simple, reproducible, and sensitive tool for assaying glycogen from tissue. In this assay, glycogen is hydrolyzed by amyloglucosidase to form β-D-glucose, which is then specifically oxidized to D-glucono-δ-lactone by glucose oxidase forming hydrogen peroxide in the process. Hydrogen peroxide, in the presence of horseradish peroxidase, reacts with 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) in a 1:1 stoichiometry to generate the highly fluorescent product resorufin which is measure at an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Cayman Chemical SPHK1 asy Buffer1X 14mL
Source Recombinant human C-terminal His-tagged coagulation factor X expressed in insect cells Amino acids 1-488 (full length) MW 52.8 kDa
Cayman Chemical MTT Cell Proliferation Assay Kit, 480 wells
Cayman’s MTT Proliferation Assay provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation. In this assay, MTT is taken up by cells and then reduced to formazan by intracellular NAD(P)H-oxidoreductases.