General Cell Based Assays
Eurofins Discoverx PathHunter eXpress NPSR1b U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0822E3CP0L)
The PathHunter NPSR1b eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptide S) based activation of NPSR1b GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPSR1b and EA-tagged β-Arrestin-2. Activation of NPSR1b stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human MMP-13 ASY
The R-Plex Human MMP-13 Assay is a multiplex immunoassay kit from Meso Scale Diagnostics that allows for the quantitative measurement of human Matrix Metalloproteinase 13 (MMP-13) in serum plasma cell culture supernatants cell lysates tissue homogenates and other biological fluids It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay can be used to study MMP-13 levels in research areas like arthritis cancer cardiovascular disease and tissue remodeling With minimal hands-on time this kit allows for rapid profiling of MMP-13 across multiple samples
Eurofins Discoverx PathHunter Human THR alpha Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human THR alpha Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated THRα nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Mouse S1P2 (EDG5) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse S1P2 (EDG5) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mEDG5 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress GLP1R CHO-K1 GPCR Assay, 10-Plate Kit. (95-0062E2CP2L)
The cAMP Hunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter™ Human VIPR2 Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human VIPR2 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure VIPR2 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human CRHR1 Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human CRHR1 Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure CRHR1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
NEW EAST BIOSCIENCES LLC RhoA Pull-Down Activation Assay Kit, 30 Assays
The RhoA Activation Assay Kit uses configuration-specific anti-RhoA-GTP Mouse monoclonal antibody to measure RhoA-GTP levels in cell extracts or in vitro GTPγS loading RhoA activation assays. Anti-RhoA-GTP mouse monoclonal antibody is first incubated with cell lysates containing RhoA-GTP. Next, the GTP-bound RhoA is pulled down by protein A/G agarose. Finally, the precipitated RhoA-GTP is detected through immunoblot analysis using Anti-RhoA Rabbit Polyclonal Antibody.
Eurofins Discoverx PathHunter eXpress HCRTR1 Total GPCR Internalization Assay, 2pl Kit. (93-0729E3CP0M)
The PathHunter HCRTR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery KIT 2400 PHOS-AKT (T308) 5PK
MA2400 Phospho-Akt (Thr308) Whole Cell Lysate Kit (5 Pl)
Eurofins Discoverx PathHunter eXpress mGALR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0604E2MCP2L)
The PathHunter mGALR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of mGALR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGALR2 and EA-tagged β-Arrestin-2. Activation of mGALR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Waters Corp Publication, Origins of UPLC Technology
This publication documents the journey and development of Ultra Performance Liquid Chromatography (UPLC) technology. It delves into the foundational aspects and historical context that led to its creation, providing a comprehensive understanding for professionals in the field.
- Explores the foundational aspects of Ultra Performance Liquid Chromatography (UPLC) technology.
- Provides historical context for the development of UPLC.
- Offers a comprehensive understanding for professionals in liquid chromatography.
Bioassay Systems Parallel Artificial Membrane Permeability Assay-BBB Kit. Assess blood brain barrier (BBB) membrane permeability of test compounds.
For quantitative determination of blood brain barrier (BBB) membrane permeability of test compounds. Key Features: Convenient. Includes all necessary equipment to run a PAMPA plate. Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: PAMPA. Samples: Chemical compounds. Procedure: Assay takes 20 hr, hands-on time 1 hr. Kit size: 96 tests.
Eurofins Discoverx PathHunter Human Glucagon (GCG Receptor) Total GPCR Internalization Cell-Based Assay Kit (HEK 293)
The PathHunter Human Glucagon (GCGR) Stable Cell Line Total GPCR Internalization Assay (HEK 293) contains a stable clonal cell line used to measure GCGR (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Mouse MC3R β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse MC3R β-Arrestin Stable Cell Pool Assay (CHO-K1) contains a stable engineered cell pool used to measure mMC3R (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell pool vials, all required culture reagents for maintaining the cells in continuous culture, and detection reagents for running the assay.