General Cell Based Assays
ACROBiosystems Human TL1A-DR3 inhibition Kit (TR-FRET) 500 Tests
Cost effective - Sufficient quantity at a lower price, accounting for dilution and pipetting losses.Comprehensive validation - Validated with various antibody subtypes and antibody drugs.Simple and fast operation - No complicated washing steps, significantly reducing time.High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.Accurate and reliable results - High sensitivity with minimal matrix effects.High throughput capability - Supports 500 tests, ideal for high-throughput screening.Fast completion - Results in just 1 hour.
Biotium ALDH1A1 (Aldehyde Dehydrogenase 1A1) (ALDH1A1/1381), CF740 conjugate, 0.1mg/mL
Aldehyde dehydrogenase 1 family member A1 (ALDH1A1), also known as retinal dehydrogenase 1, belongs to the aldehyde dehydrogenase enzyme family, which is involved in the metabolism of alcohol. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than othe
Eurofins Discoverx PathHunter eXpress mCNR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0471E2MCP0M)
The PathHunter mCNR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of mCNR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCNR1 and EA-tagged β-Arrestin-2. Activation of mCNR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress C5AR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0557E2CP0L)
The PathHunter C5AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR50 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0648E2CP1M)
The PathHunter GPR50 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR50 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR50 and EA-tagged β-Arrestin-2. Activation of GPR50 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rhTrkA-rhP75 Functional Assay, 10pl Kit. (93-1009E3CP16L)
The PathHunter eXpress rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the rhesus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR78 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0370E2ACP1L)
The PathHunter GPR78 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR78 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR78 and EA-tagged β-Arrestin-2. Activation of GPR78 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical MitoLite™ Orange EX405, 500 test
A mitochondrial dye for live cells; ex/em = 399/550 nm
Cayman Chemical cPLA2 Assay Kit, 96 Well
Arachidonoyl thio-PC is a substrate for cPLA2 by virtue of the presence of arachidonic acid at the sn-2 position of the glycerophospholipid. Hydrolysis of the arachidonoyl thioester bond at the sn-2 position by PLA2 releases free thiol which can be detected by DTNB. This assay can be used to determine the activity of cPLA2 in purified preparations, cell cultures, or tissue homogenates that are known to contain only cPLA2. Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone. Isozyme-specific cPLA2 activity can be measured by excluding sPLA2 or inhibiting iPLA2 activities in the assay.
Molecular Devices FLIPR Calcium 6-QF Assay Kit, Quench-Free, Sample, 96/384/1536-well
The Molecular Devices FLIPR Calcium 6-QF Sample Kit is a quench-free, mix-and-read fluorescent reagent set designed for sensitive intracellular calcium flux measurements. The high-quantum-yield dye increases signal amplitude for low-signal targets and the QF formulation enables assay multiplexing while minimizing quenching effects. The sample kit provides reagents sufficient for two microplates and supports 96-, 384-, and 1536-well formats.
- Quench-free dye formulation enables multiplexing with luminescent assays.
- High quantum yield increases signal window for low-signal targets.
- Mix-and-read protocol simplifies workflow and accelerates throughput.
- Reagents sufficient for two microplates, supporting 96-, 384-, and 1536-well formats.
- Includes component A (2 vials), component B buffer (1 bottle), and component C (2 vials).
- Reduces well-to-well variability, improving assay quality and reliability.
Apexbio Technology LLC LDH Cytotoxicity Assay Kit(Synonyms: LDH Cytotoxicity Detection Kit, LDH Release Assay Kit, Lactate Dehydrogenase Cytotoxicity Assay), 200T.
The LDH Cytotoxicity Assay Kit is a biochemical assay designed to quantitatively assess cell cytotoxicity through measurement of lactate dehydrogenase (LDH) enzyme activity released into the cell culture medium LDH an intracellular enzyme ubiquitously expressed is released extracellularly upon damage or death of the cell membrane This assay relies on LDH-mediated conversion of lactate to pyruvate coupled with NAD reduction to NADH The reaction product NADH interacts with specific substrates within the kit generating a compound measurable spectrophotometrically at 490 nm Accordingly absorbance intensity at this wavelength directly correlates with cellular damage severity Compared to radioactive chromium ( 51Cr) release assays this method provides comparable quantitative data in a safer non-radioactive format applicable for cell death cytotoxicity and drug screening research
Medchemexpress LLC PLAU/uPA Rat HEK29 10ug
PLAU/uPA proteins lack conserved residues critical for feature annotation propagation suggesting potential differences in molecular interactions in plasminogen activation and fibrinolysis regulation PLAU/uPA Protein Rat (HEK293 His) is the recombinant rat-derived PLAU/uPA protein expressed by HEK293 with C-His labeled tag
Discovery Life Sciences Gentest TransportoCellsTM
Gentest TransportoCellsTM Dog Organic Anion transporting Polypeptide 1b4 10e7 cells
Eurofins Discoverx PathHunter eXpress ACVR1C/ACVR2 Dimerization Assay, 2pl Kit. (93-1069E3CP5M)
The PathHunter ACVR1C/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. activin B) induced dimerization of ACVR1C/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR1C target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of ACVR1C/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress DRD2S (Short Isoform) CHO-K1 GPCR Assay, 2pl Kit. (95-0084E2CP2M)
The cAMP Hunter DRD2S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD2S GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD2S, a naturally Gi/Go coupled GPCR. Upon activation of DRD2S, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.