General Cell Based Assays
AnaSpec SENSOLYTE MG PHOSPHATE ASSAY
SensoLyte® MG Phosphate Assay Kit Colorimetric -1 kit. Component A: 1 mM Phosphate, Standard: 1 mL Component B: MG Reagent: 20 mL
Apexbio Technology LLC APExBIO Thioredoxin Reductase Colorimetric Assay Kit, 100 assays
This kit offers a rapid method for quantifying thioredoxin reductase (TrxR) activity. It utilizes NADPH as a coenzyme for TrxR to catalyze the reduction of DTNB, producing a yellow TNB that absorbs at 412 nm. A TrxR-specific inhibitor is included to eliminate background interference from other enzymes, ensuring high specificity in measuring TrxR activity.
- Rapid quantification of thioredoxin reductase (TrxR) activity.
- Utilizes NADPH as a coenzyme for enzymatic reactions.
- Catalyzes the reduction of DTNB to TNB, detectable at 412 nm.
- Includes a TrxR-specific inhibitor for high specificity and reduced background interference.
- Reliable for TrxR functional studies.
- Shelf life of 1 year.
- Intended for scientific research use only.
Abcam Hydrogen Peroxide Assay Kit
Hydrogen Peroxide Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc Cytotoxicity LDH Assay Kit-WST (500 TESTS) measures released LDH from dead cells and can be used with and without transferring supernatant.
Lactate dehydrogenase(LDH) is an enzyme that is present in almost all cell types and it catalyzes the oxidation of lactate to pyruvate in the presence of co-enzyme NAD+. Once cells are impaired by stress, injuries, chemicals or intercellular signals, LDH is rapidly released from the cell membrane. Thus, the measurement of the amount of released LDH from cells is one of the major methods to assess cell death. Since Dojindo's Cytotoxicity LDH Assay Kit-WST neither reflects the activity of living cells nor is harmful to cells, it allows the assay to perform in wells containing both viable and damaged cells. Cell Cytotoxicity is evaluated by multiple cell functions. Dojindo provides you useful selection guide. (https://www.dojindo.com/cell-proliferation-cytotoxicity-assay-kits-selection-guide/)
Dojindo Molecular Technologies Inc Mitophagy Detection Kit (1 set, 5UG x1) allows for mitochondria research for mammalian cells. No need for transfection, simply add Mtphagy Dye!
Mitochondria plays a crucial role in cells such as production of energy for cell viability. Mitophagy was recently found to be related to Alzheimer and Parkinson disease induced by the accumulation of depolarized mitochondria. Mitophagy serves as a specific elimination system that dysfunctional mitochondria caused by oxidative stress and DNA damage are sequestered into autophagosome, fused to lysosome and degraded by digestion. This kit is composed of Mtphagy Dye (reagent for detection of mitophagy) and Lyso Dye. Mtphagy Dye accumulates in intact mitochondria, is immobilized on it with chemical bonds and exhibits a weak fluorescence from the influence of surrounding conditions. When Mitophagy is induced, the damaged mitochondria fuses to lysosomes and then Mtphagy Dye emits a high fluorescence. To confirm the fusion of Mtphagy Dye labeled mitochondria and lysosomes, Lyso Dye included in this kit can be used.
TOPOGEN INC DNA Gyrase/Relaxed DNA Assay Kit
The E. coli DNA Gyrase and Relaxed DNA Assay Kit is designed to provide the investigator quick and specific assays for DNA gyrase. These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work. These kits are also appropriate for the researcher who is interested in large scale screening for novel anti-gyrase active agents. We matched the amount of DNA gyrase enzyme with the amount of relaxed pHOT-1 DNA to get you going and keep you going on the hunt for new actives.
BPS Bioscience Inc DR3:TL1A Inhibitor Screening Assay Kit, Size: 96 reactions
The DR3:TL1A Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of the interaction between DR3 (Death Receptor 3) and TL1A (TNF-like ligand 1A). This kit comes in a convenient 96-well or 384-well format, with biotin-labeled TL1A, purified DR3, streptavidin-labeled HRP, and assay buffer for 100 or 400 binding reactions.
Lonza Walkersville PPiLight Inorganic Pyrophosphate Assay, 500 Test
Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3), ligases (EC 6) and DNA polymerases such as DNA Polymerase theta (θ) encoded by POLQ. Benefits:Fast - Measure enzyme activity via pyrophosphate consumption in 1 hourSimple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substratesWide Detection Range - Sensitive to 0.02 µM with a linear range to 10 µMVersatile - Scalable to 96-, 384-, 1586-well formatsApplications:Measure activity of:PhosphotransferasesPyrophosphotasesCyclasesLigasesHydrolasesDNA Polymerases
Eurofins Discoverx cAMP Hunter eXpress GLP1R CHO-K1 GPCR Assay, 2-Plate Kit. (95-0062E2CP2M)
The cAMP Hunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
