General Cell Based Assays
Indigo Biosciences Human Insulin-Like Growth Factor Receptor 1 (IGF-1R) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Insulin-Like Growth Factor Receptor 1 reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against Insulin-Like Growth Factor Receptor 1. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request).
Agilent Technologies SEAHORSE XF FATTY ACID KIT
Seahorse XF Long Chain Fatty Acid Oxidation Test Kit.
Abcam Creatinine Assay Kit
Creatinine Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical MPO asy Buffer1X 1ea
A - and K1-opioid receptor antagonist (Kis 0.3 and 0.2 nM respectively) selective for - and K1-opioid receptors over the -opioid receptor (Ki 12.8 nM) reversibly inhibits electricity-induced twitches of isolated guinea pig ileal longitudinal muscle strips (IC50 48 nM) indicating opioid agonist activity inhibits endomorphin 1-induced conditioned place preference in mice at 0.3 UG/animal i.c.v. prevents decreases in urine output induced by fentanyl D-propoxyphene buprenorphine profadol or bromadoline in water-loaded rats at 40 mg/kg reduces hemokinin 1-induced increases in the latency to withdrawal in the tail-flick test in mice
Meso Scale Discovery R-PLEX Human Enolase 2 ASY
The R-PLEX Human Enolase 2 Assay Kit from Meso Scale Discovery is used to quantitatively measure enolase 2 in human serum plasma and cell culture supernatants It utilizes an electrochemiluminescent detection method and provides high sensitivity and broad dynamic range The assay takes approximately 4 hours to complete and requires an MSD instrument such as QuickPlex QuickPlex SQ 120 or QuickPlex SQ 240 Each kit provides reagents for one 96-well plate which can assay 40 samples in duplicate
Meso Scale Discovery R-PLEX Mouse G-CSF ASY
The R-PLEX Mouse G-CSF Assay is a multiplex immunoassay panel that measures G-CSF in mouse samples such as serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and is designed for use on Meso Scale Discovery (MSD) instrumentation platforms The assay allows sensitive accurate and rapid quantification of G-CSF levels It requires a small sample volume and provides a dynamic range of up to 3 logs The panel is provided in a convenient all-in-one kit containing plates coated with capture antibodies detection antibodies calibrators and controls The assay takes approximately 3 5 hours to complete
Cayman Chemical NFAT InhIbItr 10mg
A peptide inhibitor of NFAT; binds to the NFAT recognition site on calcineurin (IC50 = 25 pM) but does not inhibit calcineurin phosphatase activity at 100 µM; inhibits the dephosphorylation of NFAT in HEK293T lysates expressing NFAT1, -2, or -4; inhibits NFAT activation in RAW 264.7 macrophages, EA.hy926 endothelial cells, and isolated mouse thoracic aorta VSMCs (IC50s = 29.1, 30.2, and 45.7 µM, respectively, in reporter assays)
Cayman Chemical NFAT InhIbItr 1mg
A peptide inhibitor of NFAT; binds to the NFAT recognition site on calcineurin (IC50 = 25 pM) but does not inhibit calcineurin phosphatase activity at 100 µM; inhibits the dephosphorylation of NFAT in HEK293T lysates expressing NFAT1, -2, or -4; inhibits NFAT activation in RAW 264.7 macrophages, EA.hy926 endothelial cells, and isolated mouse thoracic aorta VSMCs (IC50s = 29.1, 30.2, and 45.7 µM, respectively, in reporter assays)
Sigma Aldrich Fine Chemicals Biosciences Rap1 Assay Reagent Ral GDS
Product Source expressed in E. coli
Revvity Health Sciences Inc LANCE Ultra Phospho-STAT5 (Tyr694/699) Detection Kit, 10,000 Assay Points
LANCE Ultra Phospho-STAT5 (Tyr694/699) Detection Kit, 10,000 Assay Points
Sigma Aldrich Fine Chemicals Biosciences Proteasome 20S Activity As
Protein degradation occurs most commonly through two major mechanisms lysosomal degradation and proteasome mediated degradation. Lysosomal degradation occurs through proteolytic enzyme activity and is fairly non-specific. Proteasomal degradation is targeted for a specific protein through the ubiquitination of the target protein. The target protein is typically poly-ubiquitinated and activates the 26S proteasome toward proteolysis. The proteasome 26S is the most common form of the proteasome and is an ATP-dependent proteolytic complex which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps.The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis DNA repair endocytosis and cell cycle and has been implicated in disease states such as certain types of cancer diabetes mellitus and Alzheimer disease among others making it the target f