General Cell Based Assays
BPS Bioscience Inc CBL-B TR-FRET ASSAY KIT
Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B) is an E3 ubiquitin-protein ligase that functions as a negative regulator of T-cell activation. The CBL-B TR-FRET Assay Kit is designed to measure CBL-B auto-ubiquitination activity in a homogeneous 384 reaction format.
AURORA BIOLABS LLC Cov-19 NP Rabbit Binding Assay, 384 rxn
SARS-CoV-2 Nucleocapsid protein (NP) is one of the core components of SARS-CoV-2 virus. It forms a complex with viral genomic RNA in a helical symmetrical structure and plays a key role in the process of virus replication and assembly. Since NP is abundantly expressed during infection, it can be used as an important diagnostic marker for COVID-19 and also can be used as a potential drug target or developing vaccines. Terbium-labeled anti-Tag1 antibody serves as fluorescence donor, that binds to the His-Tagged NP. If a test rabbit antibody binds to NP, fluorescence- labeled anti-rabbit antibody (fluorescence acceptor) will be brought in close proximity with the fluorescence donor. Excitation of Terbium (340 nm) generates fluorescence resonance energy transfer (FRET) to the fluorescence-labeled acceptor, which consequently fluoresces at 665 nm (figure below). Thus, the test antibody binding to NP can be quantitively measured by calculation of the fluorescent ratio of 665 nm/620 nm.
Meso Scale Discovery U-PLEX Human Fas (soluble) Assay, 96-well plate, 0.22 pg/mL LLOD, Dynamic Range: 0.22 - 1600 pg/mL, for serum and plasma, Mouse Monoclonal antibodies, Research use only
The U-PLEX Human Fas (soluble) Assay by MESO SCALE DISCOVERY is a sophisticated immunoassay designed for the quantitative detection of soluble Fas in human serum and plasma. This assay utilizes validated components of the U-PLEX platform, simplifying the development of sensitive assays suitable for future multiplexing within Immuno-Oncology analytes. Intended solely for research purposes, this product ensures reliable results in oncological and immunological applications.
- Designed for quantitative detection of soluble Fas.
- Suitable for serum and plasma samples.
- Dynamic range of 0.22 - 1,600 pg/mL.
- Sensitivity level of 0.22 pg/mL.
- Compatible with multiple instruments.
- Incorporates both capture and detection antibodies from mouse monoclonal sources.
- Part of Immuno-Oncology Group 1 multiplex compatibility.
- Multi-component storage conditions may apply.
Crystal Chem Inc Mouse Glucose Assay Kit, 10-Kit Pack, 96-tests per well
The Mouse Glucose 10-kit pack is an economical option for high-volume users of our Mouse Glucose Assay Kit offered as ten individual kits.Highlights: Easy-to-use, results in 5 minutes. Kits use a sample size of only 2 µL. For use with serum, plasma, and urine. Designed for mouse samples. Crystal Chem is a worldwide leading supplier of mouse and rat ELISA kits for research use. Our kits are among the highest quality found in the market, and are used by leading research universities, pharma companies, and government institutions around the world.
Amsbio LLC Oris Universal Cell Migration Assembly Kit - FLEX
Oris™ Universal Cell Migration Assembly Kit - FLEX
Eurofins Discoverx PathHunter eXpress MC3R Total Internalization Assay, 2pl Kit. (93-0837E3CP0M)
The PathHunter MC3R Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of MC3R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged MC3R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MC3R receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGIR Total Internalization Assay, 2pl Kit. (93-0838E3CP0M)
The PathHunter PTGIR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGIR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGIR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGIR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MC3R Total Internalization Assay, 10pl Kit. (93-0837E3CP0L)
The PathHunter MC3R Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of MC3R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged MC3R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MC3R receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CALCRL-RAMP1 (CGRP1) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0269E2CP0M)
The PathHunter CALCRL-RAMP1 (CGRP1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. β-CGRP) based activation of CALCRL GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCRL and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP1. Activation of CALCRL stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology Cathepsin B Rabbit pAb
This gene encodes a member of the C1 family of peptidases. Alternative splicing results in multiple transcript variants, with at least one encoding a preproprotein that is processed into cathepsin B light and heavy chains, which dimerize to form the active enzyme. Cathepsin B is a lysosomal cysteine protease with endopeptidase and exopeptidase activity, involved in protein turnover. It also acts as amyloid precursor protein secretase, with incomplete APP processing linked to Alzheimers disease. Overexpression of this protein is associated with esophageal adenocarcinoma and other cancers. Both cathepsin B and L are involved in cleaving the spike protein of SARS-CoV-2 during cell entry. Multiple pseudogenes of this gene have been identified.
Cayman Chemical NFAT AcTVatIon InHBTr III 50mg
A cell-permeant inhibitor of NFAT signaling; binds calcineurin (Kd = 0.8 µM), preventing it from dephosphorylating NFAT