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Sircol; Dye Reagent (3 x 110 ml). Contains Sirius Red in picric acid and has been formulated for specific binding to collagen under the conditions defined in the Sircol Manual. Sircol™ Collagen Assay component. Biocolor Item # S1005
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TypeColorimetric assayApplicationsCell culture and/or animal studiesShippingAt ambient temperature. Upon receipt, store the product at the temperature recommended below.Storage/ExpirationAll components are stable for one year, (from Invoice Date), when stored at 15–25°C. The glass vial containing collagen standard should be stored at +4°C once opened. Calibration Range2.5 – 12.5 μg using 250μl of Alkali Reagent to recover the collagen bound dye10 – 50 μg using 1000μl of Alkali Reagent to recover the collagen bound dyeLimit of Detection1.0 μg
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Bradford Protein Assay Kit is based on the Bradford method a colorimetric protein quantification approach leveraging Coomassie Brilliant Blue G-250 Under acidic conditions this dye interacts specifically with basic and aromatic amino acid residues notably arginine causing a measurable shift of its absorbance maximum from 465 nm to 595 nm accompanied by a clear solution color change to blue The absorbance intensity at 595 nm directly correlates with protein concentration enabling rapid quantitative analysis Due to its simplicity sensitivity (detection down to 0 5 g) and reliable linearity within 0 1 1 5 mg/mL range it is widely applicable in biomedical protein research
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The R-PLEX Human BCA-1 (BLC) Assay from Meso Scale Diagnostics is a multiplex immunoassay kit that allows for the simultaneous quantitative measurement of B-Cell Activating Factor (BAFF also known as B Lymphocyte Stimulator (BLyS)) in human serum plasma and cell culture supernatants This electrochemiluminescent assay utilizes MSD s proprietary SECTOR Imager and provides a sensitive and reproducible method for measuring BAFF levels with a detection range of 1 95 - 1000 pg/mL The assay requires 50 L of sample and can be completed in under 5 hours This product could be useful for research related to autoimmune diseases immune monitoring or biomarker discovery and validation
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For quantitative determination of total protein. Key Features: Fast and sensitive. Assay takes 10 min. Linear detection range of 5 - 200 mg/dL protein. Robust and Reliable. Substances in urine have minimal interference (<2%). Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD600nm (Pyrogallol Red). Samples: Urine, cerebrospinal fluid etc. Species: All. Procedure: Assay takes 10 min. Kit size: 1000 tests. Detection limit: 5 mg/dL.
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Pronase (Streptomyces griseus) is a commonly used digestive enzyme. In formalin-fixed paraffin-embedded tissues, certain antibody protocols require enzyme pretreatment for proper immunohistochemical staining. CAREZYME III is an aggressive enzyme and can be used at room temperature or at 37°C. Certain antibodies such as EGFR require this type of aggressive enzyme digestion. This product is provided as a two component system and can be used at different concentrations. CAREZYME III is not compatible with antibodies AE1/AE3 or AE3. It can also be used on an automated staining system.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More