Total Protein Assay Kits
BioVendor, LLC Sircol Soluble Collagen Assay, Bovine Collagen (Large Economy Kit Size: 440 assays)
Sircol Soluble Collagen Assay, Bovine Collagen (Large Economy Kit Size: 440 assays). Multispecies colorimetric assay. The Sircol Assay is a dye-binding method for the analysis of acid and pepsin-soluble collagens. Assess the rate of newly synthesised collagen produced during periods of rapid growth and development. New collagen is also generated during inflammation, wound healing & tumour development. The Sircol Assay is suitable for monitoring collagen produced in situ or during in-vitro cell culture and in-vitro extracellular matrix, (ECM), formation. Mammalian collagens; Types I to V can be measured. Sircol Dye Reagent contains Sirius Red in picric acid and has been formulated for specific binding to collagen; does not discriminate between collagen types, binding to the [Gly-X-Y]n helical structure as found in collagen. Not suitable for covalent cross-linked collagen (requires INSOLUBLE version of kit). Detection Limit: 1.0 g. Assay-Time: 1.5 hours. Biocolor Item # S5000
Cayman Chemical MIcro BCA Reagent 1 15mL
Micro BCA Reagent 1 has been tested and formulated to work exclusively with Cayman’s Micro BCA Protein Assay Kit (Item No. 760200).
Bioworld BCA Protein Assay Reagent A, 500 mL
BCA (Bicinchoninic Acid) Assay Reagent A A BCA Protein assay kit (cat# 20831001-1) component for use in protein quantification assays.
Medchemexpress LLC BCA-1/CXCL13 protein, rat (His-GST) | >90.0% | 20UG
Recombinant rat BCA-1 (CXCL13) is a CXC chemokine produced with an N-terminal His-GST tag in Escherichia coli and supplied lyophilized for research use. The protein is suitable for receptor binding studies, chemotaxis assays, and antibody generation, and is provided with characterized purity, molecular weight, and storage recommendations.
- Species: rat
- Tag: N-terminal His-GST
- Expression system: Escherichia coli
- Purity: >90.0%
- Predicted molecular weight: 36.8 kDa
- Formulation: lyophilized from 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0
- Storage: store at -20°C; stable for 2 years from receipt
- Available sizes: 20 μg, 50 μg, 100 μg; larger sizes on request
Cayman Chemical MIcro BCA Reagent 3 1mL
Micro BCA Reagent 3 has been tested and formulated to work exclusively with Cayman’s Micro BCA Protein Assay Kit (Item No. 760200).
Apexbio Technology LLC BCA Protein Assay Kit 1000T
BCA fully named Bicinchoninic Acid Assay is a protein quantification method based on the biuret reaction principle Under alkaline conditions protein peptide bonds reduce Cu2 (from copper sulfate) to Cu Subsequently BCA chelates Cu to form a purple-colored complex which strongly absorbs light at a wavelength of 562 nm The intensity of the colored complex is proportional to protein concentration as the reduction of Cu2 or the amount of Cu formed directly reflects the amount of protein present in the solution Thus protein concentration in an unknown sample can be determined by measuring absorbance This assay kit features easy operation high stability high sensitivity and excellent compatibility It can detect protein concentrations as low as 25 g/mL with a minimum detection limit as low as 0 5 g protein It requires a sample volume of only 1 20 L The assay shows good linearity in the concentration range of 50 2000 g/mL
Bioassay Systems QuantiChrom™ Total Protein Assay Kit. For quantitative determination of total protein.
For quantitative determination of total protein. Key Features: Fast and sensitive. Assay takes 10 min. Linear detection range of 5 - 200 mg/dL protein. Robust and Reliable. Substances in urine have minimal interference (< 2%). Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD600nm (Pyrogallol Red). Samples: Urine, cerebrospinal fluid etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 5 mg/dL.
ILEX LIFE SCIENCES LLC QuickZyme Soluble Collagen Assay, 96 Assays, 96-well plate format, colorimetric assay for collagen analysis (tissue culture supernatant, cellular extract, tissue homogenate samples)
The QuickZyme Soluble Collagen Assay Kit enables quantitative measurement of all types of fibrillary collagen (species independent) in a fast and simple 96-well plate format. The colorimetric assay is based on binding of the dye Sirius Red to collagen. The assay can be used to analyze soluble collagen or collagen that is solubilized by acetic acid alone or in combination with pepsin. The assay is intended for collagen analysis in tissue culture supernatants, cellular extracts, and tissue homogenates. The assay is not affected by serum up to at least 10%. Assay range: 0.5 to 10 µg/ml. Sensitivity: 0.69 ng/ml. Assay-time: < 3 hours. Store at 4*C. Collagen is one of the main components of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore measurement of collagen production is important in many disease related studies.
ProteinSimple Simple Plex Human CXCL13/BLC/BCA-1 Cartridge
Simple Plex assay for the detection of human BLC/BCA-1 in serum and plasma (EDTA/Heparin)
Abcam Soluble Collagen Assay Kit
Soluble Collagen Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC BCA-1/CXCL13 protein, cynomolgus (His tag) | 50 UG
Recombinant cynomolgus CXCL13 (BCA-1) is a chemokine that selectively attracts B lymphocytes. Supplied as a lyophilized 50 μg vial with an N-terminal His tag, the protein is expressed in E. coli and characterized for activity and purity for research use.
- Recombinant protein, cynomolgus origin.
- N-terminal His tag for purification and detection.
- Lyophilized formulation from 10 mM Tris, 250 mM NaCl, pH 8.0.
- Purity >95% as determined by reducing SDS-PAGE.
- Predicted molecular mass 10.3 kDa; apparent ~13 kDa by SDS-PAGE.
- Bioactivity: ED50 0.019 μg/mL in Ramos cell chemotaxis assay.
- Store lyophilized at -20°C; aliquot and store at -20°C or -80°C after reconstitution.
Medchemexpress LLC BCA-1/CXCL13 protein, cynomolgus (His tag) | >95.0% | 10 UG
Recombinant cynomolgus CXCL13 (BCA-1) is a His-tagged chemokine produced in E. coli for use in cell-based and biochemical assays. It acts as a B-lymphocyte chemoattractant and is supplied lyophilized with recommended reconstitution and storage instructions. Purity and bioactivity are validated by SDS-PAGE and cell migration assays.
- Recombinant cynomolgus CXCL13 (BCA-1), His-tagged.
- Expressed in E. coli.
- Purity >95.0% by reducing SDS-PAGE.
- Approximate molecular weight 13 kDa under reducing conditions.
- Lyophilized from 10 mM Tris, 250 mM NaCl, pH 8.0.
- Validated chemoattractant activity on Ramos cells (ED50 0.019 μg/mL).
- Store lyophilized at -20°C; aliquot and freeze at -20°C or -80°C after reconstitution.
Abcam BCA protein assay kit reducing agent compatible (microplate)
BCA protein assay kit reducing agent compatible (microplate)
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC C-X-C motif chemokine 13 (CXCL13), cynomolgus, His-tag | >95.0% | 500 UG
Recombinant C-X-C motif chemokine 13 (CXCL13, also BCA-1) from cynomolgus, supplied as a His-tagged, lyophilized protein for research use. The protein is expressed in E. coli, provided with low endotoxin, and validated for chemoattractant activity in cell assays.
- His-tagged recombinant protein from cynomolgus
- Expressed in E. coli and lyophilized for stability
- Purity greater than 95% by reducing SDS-PAGE
- Endotoxin level <1 EU/μg (LAL method)
- Apparent molecular weight ~13 kDa; ED50 0.019 μg/mL in chemoattraction assay
- Formulated in Tris/NaCl; recommend aliquoting with carrier protein for long-term storage at -20°C
- Suitable for cell migration, receptor binding, and signaling studies