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The EasySep™ Mouse CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-CD4+ T cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.This product replaces the EasySep™ Mouse CD4+ T Cell Enrichment Kit (Catalog #19752) for even faster cell isolations.
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MW 307.48 g/mol, Purity >98%. Detergent used in the solubilization of oleosins. Synthetic zwitterionic detergent, which unlike other amphoteric surfactants, retains its zwitterionic character over a broad pH range. This property is attributed to the presence of a strongly basic quaternary ammonium ion and an acidic sulfonate ion of equal strength. This ionic balance presumably prevents irreversible binding to either anionic or cationic compounds.
MW 318.42 g/mol, Purity >99%. Selective nuclear transport receptor importin-β inhibitor (IC₅₀ = 25 μM). Blocks SINC localization at the nuclear envelope. Impairs release of importin-β cargo and induces defects in defects in spindle assembly during mitosis.
MW 241.24 Da, Purity >99%. Non-competitive, irreversible protein tyrosine phosphatase (PTP) inhibitor (IC₅₀ values are 0.9 (PTP1B), 1.7 (TCPTP), 0.09 (SHP-2), 1.5 (LAR) and 0.7 (YopH) μM). Active in vivo and cell-permeable.