Gel Filtration Media and Columns

Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1 – 30 of 62 results
1

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

EDGE
Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type HiTrap Heparin Sepharose HP prepacked column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type DNA-binding Proteins, coagulation facto rs, Lipoproteins and Protein synthesis facto rs
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
2

Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

EDGE
Particle Size 18 to 111 μm in dry form
Height (Metric) 30cm
Flow Rate 720 cm/hr.
Content And Storage 4 to 30°C
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex LH-20
Sample Type Steroid, Terpenoid, Lipid, low molecular weight Peptide
Diameter (Metric) Inner 2.6cm
For Use With (Application) For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Support Type Hydroxypropylated, cross-linked Dextran
Max. Pressure 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Height (English) 11.81 in.
3

MP Biomedicals™ Sephadex™ G-100

Fractionation Range (MW)

4

Cytiva Superdex™ 200 Prep Grade

Produce separation medium with high selectivity and resolution.  Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D

5

Cytiva Sephadex G-50 Medium, 500g

Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.

6

Cytiva Sephadex™ G10 Media

Gel filtration medium for desalting and buffer exchange of peptides and small biomolecules

Height (Metric) 30cm
Bed Volume 2 to 3mL/g in Distilled Water
Content And Storage 4 to 30°C
pH Range 2 to 13
Diameter (Metric) Inner 2.6cm
For Use With (Application) Desalting and Buffer exchange of Peptides and small biomolecules >700 M.W.
Max. Pressure 24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d
Particle Size 55 to 165 μm in wet form, 40 to 120 μm in dry form
Flow Rate < Darcy´s Law
Format Solid Bead
Product Type Sephadex G-10
Sample Type Peptides and small biomolecules
Support Type Cross-linked Dextran
Height (English) 11.81 in.
7

Cytiva Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.

8

Cytiva Superose™ Media Prep Grade Lab Packs

High performance gel filtration media for scaleup to preparative purifications of biomolecules

Particle Size 30 to 40 μm
Flow Rate 40 cm/hr.
Bed Volume ≈100mL
pH Range 3 to 12
For Use With (Equipment) AKTA™ design pump or system
Sample Type Proteins, Peptides, Polynucleotides and wide variety of biomolecules
Quantity Each
For Use With (Application) Separations of Proteins and other biomolecules according to size
Support Type Highly Cross-linked Agarose
9

Cytiva Sephadex™ G75 Superfine Media

Classic gel filtration medium

10

Cytiva Sephadex™ G50 Medium Media

Suitable for gel filtration

Particle Size 100 to 300 μm in wet form, 50 to 150 μm in dry form
Height (Metric) 10cm
Bed Volume 9 to 11mg/g
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G-50 Medium
Sample Type DNA
Diameter (Metric) Inner 5cm
Support Type Cross-linked Dextran
Height (English) 3.93 in.
11

Cytiva Sepharose Agarose Gel Filtration Media

Choose from a wide range of bead-formed agarose-based gel filtration media. Cytiva Life Sciences™ Sepharose Agarose Gel Filtration Media shows good separation over a wide molecular weight range.

Format Solid Bead
Quantity 1L
For Use With (Application) Allow characterization or cleaning up of samples containing components of diverse molecular weight
12

Cytiva Sephadex™ G75 Media

Suitable for gel filtration

13

Cytiva Sephadex™ G-50 and G-100 DNA Grade Agarose Gels

Used for purification of DNA from small molecules.  Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.

Content And Storage 4° to 30°C
Format Beaded Gel
pH Range 2 to 10
For Use With (Equipment) Auto Seq G-50 column, ProbeQuant G-50 micro column, MicroSpin G-50 column and NICK column
Sample Type DNA
Support Type Cross-linked Dextran with Epichlorohydrin
14

Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Particle Size 34 μm
Ligand Type 10μM NHS groups/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 8°C
pH Range 3 to 12
Product Type HiTrap NHS activated HP column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
15

Cytiva HiTrap™ Streptavidin HP Column

Use for fast and easy preparative purifications either alone or connected in series. HiTrap™ Streptavidin HP Column are convenient and reliable prepacked columns.

Promotions are available
16

Cytiva HiTrap™ Benzamidine FF (high sub) Columns

Remove or purify serine proteases such as thrombin. Cytiva Life Sciences™ HiTrap™ Benzamidine FF (high sub) Columns are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub).

Particle Size 90 μm
Ligand Type >12μM p-aminobenzamidine/mL medium
Height (Metric) 2.5cm
Content And Storage 2° to 8°C
pH Range 2 to 8
Product Type HiTrap Benzamidine Sepharose 4 FF (high sub) column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Serine Proteases such as Thrombin
Material Polypropylene
For Use With (Application) Removal or purification of Serine Proteases such as Thrombin
Support Type 4% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
17

Cytiva Superdex™ 75 Prep Grade

Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins

18

Cytiva Sephacryl™ S-100 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

19

Cytiva Sephacryl™ S-200 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

20

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

21

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000.  Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

22

Cytiva HiPrep™ Sephacryl™ S-400 HR Columns

Maximum recovery and minimum nonspecific adsorption

23

MP Biomedicals™ Sephadex™ G-25

Used in affinity chromatography, protein chromatography and gel filtration chromatography. Sephadex™ G-25 is a gel filtration medium that has been used to study the acceleration of diabetic wound healing and the treatment of epithelial damage.

Flow Rate 100 to 150 cm/hr.
Content And Storage Store at Room Temperature (15°C to 30°C)
Format Powder
24

Cytiva Sephadex™ G15 Media

Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight

25

Cytiva Sephadex™ G50 Fine Media

Suitable for gel filtration

Height (Metric) 10cm
Bed Volume 9 to 11mg/g
Content And Storage 4 to 30°C
pH Range 2 to 10
Diameter (Metric) Inner 5cm
For Use With (Application) For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids
Max. Pressure min 150 cm/h, bed height 10 cm, column 5 cm i.d.
Particle Size 34 to 208 μm in wet form, 20 to 80 μm in dry form
Flow Rate < Darcy´s Law
Format Solid Bead
Product Type Sephadex G-50 Fine
Sample Type DNA
Support Type Cross-linked Dextran
Height (English) 3.93 in.
26

Cytiva HiTrap™ Blue HP Columns

For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins

Particle Size 34 μm
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 4 to 12
Product Type HiTrap Blue HP prepacked column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Albumin, interferon, a broad range of nucleotide-dependent Enzymes, a2-macroglobulin, coagulation facto rs and related Proteins
Material Polypropylene
For Use With (Application) Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
27

Cytiva HiPrep™ Sephacryl™ S-100 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Peptides and small proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
28

Cytiva HiPrep™ Sephacryl™ S-200 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Antibodies, serum proteins and mid-size proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
29

Cytiva IgG Sepharose™ 6 Fast Flow Medium

Use for rapid purification of protein A and protein A fusion conjugates. IgG Sepharose™ 6 Fast Flow Medium achieves the highest flowrates.

30

Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel

Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration