Magnetic Affinity Separation Particles
Rockland Immunochemicals Rabbit TrueBlot: Anti-Rabbit IgG HRP - 18-8816-33
Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Rabbit IgG TrueBlot is provided as 1000X solution. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the IP. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For IP, Anti-Rat IgG beads, or Anti-Rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. Specific conditions for each protein and antibody combination should be specifically optimized by the end user.
Raybiotech Inc ANTI-HUMAN MAGNETIC BEADS 4ML
Superparamagnetic nanoparticles coupled with Goat anti-Human IgG and utilized in the magnetic separation and isolation of human antibodies from serum or human antibody-labeled components.
Biolegend MojoSort™ Streptavidin Nanobeads
MojoSort™ Streptavidin Nanobeads Apps: Cell Sep - Pos, Cell Sep - Neg; Size: 1 mL
ALADDIN SCIENTIFIC CORPORATION Recombinant Human ErbB2/HER2 Protein, >90% (SDS-PAGE), high purity, 50ug
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2.
