Protein Purification Reagents and Kits
Abcam Complex I Immunocapture Kit
Complex I Immunocapture Kit
The product is subject to the following: Abcam Restricted Use Statement
Active Motif miRNA Target IP Kit
Active Motif's miRNA Target IP Kit was designed to identify the physical interactions of a miRNA with endogenous mRNA transcripts. It utilizes protein G-coupled magnetic beads and a pan-Ago antibody to efficiently immunoprecipitate miRNA/mRNA complexes that have associated with Ago1, Ago2 or Ago3 proteins as part of a RISC complex. Following their immunoprecipitation, the mRNA targets are purified and then amplified by qRT-PCR using gene-specific primers. This makes it possible to validate the specific mRNA transcripts that are binding targets of that miRNA. It is also possible to profile the immunoprecipitated mRNA molecules using conventional expression microarrays or RNA-Seq.
American Research Products Inc Rat p53 tumor protein p53 TP5
Rat p53/tumor protein p53/TP53 ELISA Kit from CUSABIO Detection Range 12 5 pg/mL-800 pg/mL Sensitivity 3 12 pg/mL Sample serum plasma tissue homogenates Method Sandwich
ABclonal Technology MAPKAPK-2/MK2 Rabbit pAb
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Active Motif ChIP-IT Express Enzymatic Shearing Kit (included in 53009)
The first step in successful chromatin immunoprecipitation is shearing the chromatin into 200-1000 bp fragments. This has traditionally been performed by subjecting the isolated chromatin to different pulses of sonication. Sonication is an effective method for shearing DNA, and is the most widely-used method. In some circumstances, however, it can be difficult to optimize due to complications arising from emulsification and overheating. Because of this, Active Motif has developed a robust and user-friendly method to shear chromatin by enzymatic treatment. The Enzymatic Shearing Kit uses a proprietary Enzymatic Shearing Cocktail that quickly shears DNA into 200-1000 bp fragments. Because enzymatic shearing is solely time and temperature dependent, the problems associated with sonication are eliminated and ChIP results are improved.
ABclonal Technology PARP1 Rabbit pAb
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Cayman Chemical Methylated LysIn PolycLNal
Immunogen: Methylated KLH • Host: Rabbit • Species reactivity: (+) Species independent • Applications: ELISA, ICC, IF, IP, WB • Lysine can be methylated once, twice, or three times by lysine methyltransferases. The transfer of methyl groups from SAM to histones is catalyzed by histone methyltransferases.
Medchemexpress LLC p53 Rat His 10ug
Recombinant full-length rat p53 protein (M1-D391), expressed in Escherichia coli and supplied lyophilized with an N-terminal 6xHis tag. The protein is formulated for stability and provided at >90% purity for use in biochemical assays, antibody validation, protein-protein interaction studies, and assay development. Store frozen and follow the datasheet for reconstitution and handling to preserve activity.
- Full-length sequence M1-D391, matching accession P10361.
- N-terminal 6xHis tag for purification and detection.
- Expressed in Escherichia coli for high-yield production.
- Lyophilized formulation with stabilizing excipients for storage stability.
- Purity greater than 90% as determined by reducing SDS-PAGE.
- Suitable for biochemical assays, antibody validation, and interaction studies.
- Recommended to aliquot and store at -20°C or -80°C to minimize freeze-thaw.
Proteintech Group Inc V5-Trap Magnetic Particles M-270
The ChromoTek V5-Trap Magnetic Particles M-270 consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
Cell Signaling Technology SimpleChIP Plus Enzymatic Chromatin IP Kit (Agarose Beads) 1 Kit
SimpleChIP Plus Enzymatic Chromatin IP Kit (Agarose Beads) 1 Kit
Cellecta Inc TET-ACTVTD DOX-ON NO RFP SNSR
InDOXible™ Tet-Activated cDNA Cloning Vector (Single-Vector Tet System)Dox-On No RFP Sensor
Proteintech Group Inc DYKDDDDK Fab-Trap™ Agarose Kit
The ChromoTek DYKDDDDK Fab-Trap™ Agarose Kit consists of an anti-DYKDDDDK-tag Fab fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose Kit is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc
