Protein Purification Reagents and Kits
Genscript Corporation MonoRab Anti-DYKDDDDK Magnet
GenScript MonoRab sup TM /sup Anti-DYKDDDDK Magnetic Beads are super paramagnetic beads with diameter of 70 m covalently coated with a rabbit monoclonal anti-DYKDDDDK antibody The beads are supplied as 50% slurry in TBS with 50% glycerol and 0 02% sodium azide The MonoRab sup TM /sup Anti-DYKDDDDK Magnetic Beads have a binding capacity of 1 5 mg DYKDDDDK-tagged protein (Size 50KDa) per 1 ml settled beads The beads can be re-used for at least 5 times Based on our new rabbit monoclonal antibody technology (MonoRab sup TM /sup ) this beads offers high binding capacity high purity and withstand stringent washing steps especially with buffers containing high concentration of salt The beads are resistant to up to 2 M NaCl and to buffers with pH as low as 2 5
Bioworld Co-IMAC 5 x 1mL Columns, 5 Column
Co-immobilized metal affinity chromatography (IMAC) columns consist of Co-Chelated Separoporematrix conveniently prepacked spin columns for gravity flow chromatography.Co-IMAC Columns separate proteins via their differences in affinity toCo2+ metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.Technical SpecificationsImminodiacetate immobilized matrix charged withCo metal ions.Chelating group coupling method: epoxy activationMatrix: cross-linked Separopore 4BParticle size (range): 50-170µm Metal ion capacity: > 20µmol Cu2+/ml gelSpacer arm: 14 atompH stability: 3-12Binding Capacity: Approx. 40 mg His-tagged protein/ml mediumSupplied as suspension in 20% ethanol.
Bioworld Zn-IMAC 5 x 2mL Columns, 5 Column
Zn-immobilized metal affinity chromatography (IMAC) columns consist of Zn-Chelated Separoporematrix conveniently prepacked spin columns for gravity flow chromatography.Zn-IMAC Columns separate proteins via their differences in affinity toZn2+ metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.Technical SpecificationsImminodiacetate immobilized matrix charged withZn metal ions.Chelating group coupling method: epoxy activationMatrix: cross-linked Separopore 4BParticle size (range): 50-170µm Metal ion capacity: > 20µmol Zn2+/ml gelSpacer arm: 14 atompH stability: 3-12Binding Capacity: Approx. 40 mg His-tagged protein/ml mediumSupplied as suspension in 20% ethanol.
Gold Biotechnology Inc L-Glutathione, Reduced 25 g
L-Glutathione is a co-substrate to glutathione transferase found in plants and animals and is involved in the elimination of free radicals resulting from both cellular respiratory and metabolic activities.This antioxidant plays a role in reducing inflammatory stress in plasma and lymphocytes by directly reacting with reactive oxygen species (ROS). It also serves in amino acid transport and the maintenance of protein sulfhydryl reduction.
ILEX LIFE SCIENCES LLC EndoTrap HD 5/1, 5 mL resin column, Affinity chromatography endotoxin (LPS) removal kit
EndoTrap HD endotoxin removal products, manufactured in Germany by LIONEX, are intended for in vitro quantitative removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts, phage, and nucleic acids. EndoTrap HD can be used in small scale processes for R&D and large scale manufacturing processes. It can be applied in early or late biomanufacturing process steps. EndoTrap HD is based on hydrophilic, dimensionally stable affinity matrix with excellent pressure/flow characteristics.Method: affinity chromatography.Format: pre-filled gravity flow columns, pre-filled FPLC columns, or bulk resin.LPS Binding Capacity: > 5,000,000 EU/mL.LPS Removal Rate: > 99.99%.Sample Recovery Rate: > 95%.pH Sample Range: 4-10.Non-toxic, no cytotoxic effects.Re-use more than 10 times.Suitable for high-salt conditions.The EndoTrap HD Leakage ELISA is available for quantitative detection of leached ligand from EndoTrap HD resin.
World Precision Instrument WPI MICRO-ePORE™ Pinpoint Cell Penetrator
Pinpoint Cell Penetrator for targeted cell microinjection and increased embryo viability
The new WPI MICRO-ePORE™ pinpoint cell penetrator is a simple and versatile system that can be used for efficient cell microinjection of a diverse array of compounds and biomolecules into oocytes and pre-implantation stage mammalian embryos. Patent pending Flutter Electrode Technology assists in small, clean, precise membrane penetration without tearing or damaging the membrane when you are working on the microinjection of transgenic animals or cell manipulation.
Platinum wire electrode holders (sold separately) are available!
NOTE: Microinjection pump and other accessories shown in the system image are sold separately.
STEMCELL Technologies RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Memory CD4+ T Cell Isolation Kit is designed to isolate pan-memory CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-memory CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD45R, CD45RB, CD49b, CD105 and TCRγ/δ) and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.Learn more about our next-generation EasySep™ mouse cell isolation kits (/easysep-15-minute-kits), featuring RapidSphere™ technology.
AdipoGen IGFL4 human rec His
Recombinant Protein. Human IGFL4 aa 20-124 is fused at the N-terminus to a His-tag. Endotoxin <0.01EU/µg purified protein LAL test.. Lyophilized. Contains PBS. The Insulin-growth factor-like gene family is a new family of proteins consisting of four proteins in humans IGFL1 to 4 and one in mice mIGFL. mIGFL is expressed in normal skin in mice and further upregulated during inflammation responses in the skin or after skin wounding. In humans only IGFL1 expression is increased in psoriatic skin samples. mIGFL and human IGFL1, 3 and 4 interact with specificity and high affinity to a novel receptor named IGF-like family receptor 1 formerly TMEM-149. Analysis of the amino acid sequence of IGFLR1 indicated that this receptor is likely a novel member of the TNF-R family. IGFLR1 is expressed most abundantly on mouse T cells, suggesting that mIGFL and IGFL1 produced in the skin may potentially exert regulatory functions on T cell responses.
Amsbio LLC Exo-FACS ready to use kit for analysis of exosomes markers from cell media
Exo-FACS ready to use kit for analysis of exosomes markers from cell media
Revvity Health Sciences Inc AlphaLISA Anti-6His Acceptor Beads, 5 mg
AlphaLISA Anti-6His Acceptor Beads, 5 mg
ILEX LIFE SCIENCES LLC EndoTrap HD 10, 10 mL settled resin, Affinity chromatography endotoxin (LPS) removal resin
EndoTrap HD endotoxin removal products, manufactured in Germany by LIONEX, are intended for in vitro quantitative removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts, phage, and nucleic acids. EndoTrap HD can be used in small scale processes for R&D and large scale manufacturing processes. It can be applied in early or late biomanufacturing process steps. EndoTrap HD is based on hydrophilic, dimensionally stable affinity matrix with excellent pressure/flow characteristics.Method: affinity chromatography.Format: pre-filled gravity flow columns, pre-filled FPLC columns, or bulk resin.LPS Binding Capacity: > 5,000,000 EU/mL.LPS Removal Rate: > 99.99%.Sample Recovery Rate: > 95%.pH Sample Range: 4-10.Non-toxic, no cytotoxic effects.Re-use more than 10 times.Suitable for high-salt conditions.The EndoTrap HD Leakage ELISA is available for quantitative detection of leached ligand from EndoTrap HD resin.
STEMCELL Technologies RoboSep™ Mouse Plasmacytoid DC Isolation Kit, For processing: 2 x 10^9 cells
The EasySep™ Mouse Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-pDCs. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™, For processing: 100 mL whole blood processed
The EasySep™ Direct HLA Crossmatch B Cell Isolation Kit can be used to isolate highly purified B cells directly from human whole blood, spleen, or lymph nodes by immunomagnetic negative selection. This cell isolation kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and and are immediately available for downstream applications such as flow cytometry crossmatch.
STEMCELL Technologies EasySep™ Mouse Mesenchymal Stem/Progenitor Cell Enrichment Kit, For processing: 4 x 10^8 cells
The EasySep™ Mouse Mesenchymal Stem/Progenitor Cell Enrichment Kit is designed to isolate mesenchymal stem/progenitor cells from mouse compact bone by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-mesenchymal stem/progenitor cells (CD45, TER119). Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.