Protein Purification Reagents and Kits
LifeSensors Tandem Ubiquitin Binding Entities (TUBE2) TAMRA 50µg
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitylated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” protein in its polyubiquitin state. TAMRA-TUBE 2 has a single TAMRA fluorophore (Exc. = 540 nm, Emm. = 578 nm) attached to the fusion tag of TUBE 2 (Cat. No. UM202). Since the fluorophore is attached to the tag, it does not affect binding of the tandem ubiquitin binding domains to polyubiquitin chains. This product is protected by one or more US or Foreign patents.
Enzo Life Sciences Puromycin . 2HCl, (50mg), CAS Number: 58-58-2
Protein synthesis inhibitor. Inhibits protein synthesis. Inhibits translation by causing premature release of nascent polypeptide. Induces apoptosis. Alternative Name: 3'-[[2-Amino-3-(4-methoxyphenyl)-1-oxopropyl]amino]-3'-deoxy-N,N-dimethyl-adenosine . 2HCl, 3'-(alpha-Amino-p-methoxy-hydrocinnamamido-3'-deoxy-N,N-dimethyl-adenosine . 2HCl. Formula: C22H29N7O5 . 2HCl. MW: 544.4. CAS Number: 58-58-2. MI: 14: 7943. Purity: ≥98% (HPLC, TLC). Appearance: White to off-white solid. Solubility: Soluble in water (50mg/ml). Use/Stability: Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 3 months at -20°C. Long Term Storage: -20°C
Tosoh Bioscience LLC TSKGEL G3000PWXL 7.8X300
Column, Size Exclusion Chromatography (SEC), Aqueous and Organic; Tosoh TSKgel G3000PWxl; Media: Polymer; Particle: 6 micrometer; Pore Size: 200 angstrom; 7.8mm I.D. x 30cm Length; Stainless Steel - High Resolution XL
Tosoh Bioscience LLC TSKGEL OLIGODNA RP 4.6X150
Column, Reversed Phase Chromatography; Tosoh TSKgel OligoDNA RP; Media: Silica; Particle: 5 micrometer; Pore Size: 250 angstrom; 4.6mm I.D. x 15cm Length; Stainless Steel - Standard
MilliporeSigma Total Protein Extraction Kit, 1 kit
Total protein extraction kit is an excellent tool for the initial purification and preparation of proteins from any tissues and cells. Total protein isolated by this kit is native and can be used for downstream applications.
Immunology Consultants anti-DYKDDDDK (Flag) Antibody Rabbit - HRP Conjugated
RFLG‑45P is an HRP‑conjugated rabbit antibody specific for the DYKDDDDK (FLAG) tag, designed for chemiluminescent and colorimetric detection workflows. The horseradish peroxidase label enables highly sensitive signal generation in ELISA, Western blotting, and other enzyme‑based assays. Affinity purification ensures strong specificity and minimal nonspecific binding, supporting consistent and reproducible results. Produced by Immunology Consultants and supplied in a 1 mg format, RFLG‑45P is a powerful reagent for robust, high‑sensitivity FLAG detection.
Tosoh Bioscience LLC TSKGEL OCTADECYL-4PW 4.6X150
Column, Reversed Phase Chromatography; Tosoh TSKgel Octadecyl-4PW; Media: Polymer; Particle: 7 micrometer; Pore Size: 500 angstrom; 4.6mm I.D. x 15cm Length; Stainless Steel
Immunology Consultants anti-DYKDDDDK (Flag) Antibody Rabbit - Affinity Purified
RFLG‑45A is an affinity‑purified rabbit polyclonal antibody targeting the DYKDDDDK (FLAG) epitope, widely used in recombinant protein expression and detection. The purification process yields high‑specificity antibodies with minimal background, supporting clear and reproducible results in Western blotting, ELISA, immunoprecipitation, and immunofluorescence. This reagent provides strong, selective binding to FLAG‑tagged proteins, ensuring dependable performance across diverse assay conditions. Manufactured by Immunology Consultants and supplied in a 1 mg format, RFLG‑45A is an essential tool for researchers working with FLAG‑tagged constructs.
Zymo Research Corporation M-Solubilization Buffer (4.5 ml)
The M-Solubilization Buffer is designed for use in the preparation of the CT Conversion Reagent in the EZ DNA Methylation-Direct Kit. The M-Solubilization Buffer facilitates the solubilization of the CT Conversion Reagent from a solid mixture into a liquid reagent. Highlights: Sold separately or as part of the EZ DNA Methylation-Direct Kits.
Zymo Research Corporation EZ DNA Methlyation-Direct™ Kit (50 Rxns)
The EZ DNA Methylation™-Direct™ Kit is a further refinement of our popular EZ DNA Methylation™ and EZ DNA Methylation™-Gold™ kits. These products feature reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of these kits make it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation™-Gold™ kits, DNA denaturation and bisulfite conversion processes are combined into a single step. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
Enzo Life Sciences Poly(ADP-ribose), standard (1µg)
Alternative name: PAR. Purity: ≥95% (HPLC of ADP-ribose RNA and DNA measurement). MW: 1’100 to 275’000 depending on the number of units of ADP-ribose. Formulation: Liquid. In water. Source: Released from automodified PARP-1 by base treatment and purified by boronate affinity. Long Term Storage: -80°C. Use/Stability: Stable for one week when stored at +4°C. Stable for at least 2 years after receipt when stored at -80°C. Handling: Avoid freeze/thaw cycles.
Antibodies Online MagSi-S Epoxy 3.0 Beads, 2ml
PurposeSuperparamagnetic silica particles with epoxy modified surface. Intended for epoxy coupling chemistry to enzymes and other NH2 containing molecules.Bead Size - 3 µmCharacteristicsCoating chemistry: EpoxyBead concentration: 1 - 3 x 10^9 beads/mLComponentsMagnetic silica beads with activated surface