Protein Purification Reagents and Kits
Active Motif ChIP-IT PBMC
Active Motif's ChIP-IT PBMC Kit overcomes the challenges of generating quality chromatin for use in ChIP from peripheral blood mononuclear cells (PBMCs). PBMCs, including lymphocytes (T cells, B cells and NK cells), monocytes and dendritic cells, constitute a critical component of the peripheral immune system and are often used in immunology, cancer and infectious disease research. However, for researchers who want to perform ChIP, PBMCs make it difficult to extract quality chromatin because they are highly resistant to lysis under conditions normally suitable for other cell types. Active Motif leveraged its years of experience working with these difficult-to-lyse cells into developing a kit to finally enable researchers to prepare chromatin and perform ChIP on PBMCs.
Bio Rad Laboratories INLINE FILTER KIT
Kit, Inline filter; Bio-Rad; For use w/BioLogic DuoFlow workstation replacement and maintenance parts; Includes 1 inline filter, 2 replacement frits
Genscript Corporation Ni Resin FF
GenScript Ni Resin FF is a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni sup 2 /sup by three coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins
Genscript Corporation Ni Resin FF
GenScript Ni Resin FF is a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni sup 2 /sup by three coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins
Genscript Corporation Anti-DYKDDDDK G1 Affinity Resi
GenScript Anti DYKDDDDK G1 Affinity Resin (Cat No L00432) is designed for the purification of DYKDDDDK tagged protein from commonly used protein expression systems including bacteria yeast and mammalian cells
STEMCELL Technologies EasySep™ Human Memory CD4+ T Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Memory CD4 T Cell Enrichment Kit is designed to isolate memory CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD8, CD14, CD16, CD19, CD20, CD36, CD45RA, CD56, CD123, TCRγ/δ, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Biotage BX PHYTIP COL W/PROPLUS AFF RS
Box of 96 PhyTip columns (1 ml volume) containing 80 μl of ProPlus affinity resin per column
Sigma Aldrich Fine Chemicals Biosciences Glutathione SepharoseR 4B
Glutathione Sepharose(R) 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose(R) 4B. Proteins are eluted under mild non-denaturing conditions that preserve protein antigenicity.
STEMCELL Technologies RoboSep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II is designed to isolate CD4+CD25+ T cells from single-cell suspensions of splenocytes or other tissues by negative followed by positive selection. CD4+ T cells are first enriched by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Cocktail. CD25+ cells are then positively selected from the pre-enriched cells using EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail.
STEMCELL Technologies EasySep™ Human Naïve Pan T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Naïve Pan T Cell Isolation Kit is designed to isolate naïve pan T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Agilent Technologies GlykoPrep-plus N-glycan prep with InstantAB, no labware, 96-ct
GlykoPrep-plus N-glycan prep with InstantAB, no labware, 96-ct.
STEMCELL Technologies EasySep™ Release Human PE Positive Selection Kit, Size: 1 x 10^9 cells
Immunomagnetic positive selection of particle-free human cells labeled with PE-conjugated antibodies
STEMCELL Technologies EasySep™ Mouse Biotin Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556). For highly purified particle-free cells, we recommend the EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655). (https://www.stemcell.com/products/easysep-release-mouse-biotin-positive-selection-kit.html)
STEMCELL Technologies RoboSep™ Human CD56 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD56 Positive Selection Kit II is designed to isolate CD56+ cells from fresh or previously frozen peripheral blood mononuclear cells and human skeletal muscle (myoblasts and fibroblasts) cultures by positive selection. Desired cells are targeted with antibody complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells, NKT cells and human myoblasts.This product replaces the EasySep™ Human CD56 Positive Selection Kit (Catalog #18055) for even faster cell isolations.