Protein Purification Reagents and Kits
Cayman Chemical NavenIFlex TIsue MR RED 100RXN
A metabolite of isoleucine induces the formation of TBARS in isolated rat cerebral cortex at 0.5-5 mM elevated levels of are associated with 2-MBCDD also known as SBCAD
STEMCELL Technologies EasySep™ RBC Depletion Reagent for RoboSep, For processing: 100 mL whole blood processed
The EasySep™ RBC Depletion Reagent is designed to immunomagnetically deplete red blood cells (RBCs) from fresh human peripheral blood, umbilical cord blood, bone marrow or leukapheresis samples by negative selection. RBCs are targeted with Tetrameric Antibody Complexes recognizing Glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off.This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Human Biotin Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553). For highly purified particle-free cells, we recommend the EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653). (https://www.stemcell.com/products/easysep-release-human-biotin-positive-selection-kit.html)
STEMCELL Technologies RoboSep™ Mouse APC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse APC Positive Selection Kit (Catalog #18452).
STEMCELL Technologies RoboSep™ Human Naïve B Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human Naïve B Cell Enrichment Kit is designed to isolate naïve B cells from peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-naïve B cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube.
Genscript Corporation High Affinity Ni-Charged Resin
GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2 by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins
BIOHIPPO AAV-EF1a-DIO-TVA(T66)-EGFP-WPRE-hGH-polyA
Recombinant adeno associated virus expressing TVA fused to EGFP under the EF1a promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
STEMCELL Technologies RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail, For processing: 40 mL bone marrow processed
The RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail is designed to pre-enrich progenitor cells by removing lineage-specific cells from whole bone marrow by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD11b, CD14, CD16, CD19, CD56, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The pre-enriched progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ Mouse B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-B cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using and EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For isolation of B cells expressing CD11b or CD43, we recommend using the EasySep™ Mouse Pan-B Cell Isolation kit (Catalog #19844 (https://www.stemcell.com/products/easysep-mouse-pan-b-cell-isolation-kit.html)).This product replaces the EasySep™ Mouse B Cell Enrichment Kit (Catalog #19754) for even faster cell isolations.
STEMCELL Technologies EasySep™ Mouse Pan-ILC Enrichment Kit, For processing: 1 x 10^9 cells
This product is designed to enrich group 1, 2 and 3 innate lymphoid cells (ILC1, ILC2 and ILC3s) from single-cell suspensions of mouse tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-ILCs and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry and cell sorting.
STEMCELL Technologies EasySep™ HLA Total Lymphocyte Enrichment: Complete Processing Kit for Whole Blood, For processing: 200 mL whole blood processed
The EasySep™ Total Lymphocyte Enrichment Kit: Complete Processing Kit for Whole Blood is designed to isolate human lymphocytes from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD14, CD15, CD16, CD33, CD36, CD41, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Direct Human CD4+ T Cell Isolation Kit for RoboSep™, For processing: 100 mL whole blood processed
Isolate CD4+ T cells directly from human whole blood by immunomagnetic negative selection. Desired cells undergo minimal manipulation and are untouched and highly purified.This product labels RBCs, platelets and unwanted cells with antibody complexes and magnetic particles. The magnetically labeled RBCs, platelets and non-target cells are separated from the untouched CD4+ T cells in an EasySep™ magnet and poured or pipetted into a new tube.Isolated cells are available for use in downstream uses such as flow cytometry, functional assays or culture.
Lonza Walkersville Hu CD4/CD45RA Naive T-Cells
Na ve CD4 T cells are negatively selected using a two step isolation process CD4 T Cells are first negatively selected from mononucleated cells obtained from leukapharesed blood using an indirect immunomagnetic CD4 labeling system CD45RO microbeads are then used to deplete the CD45RO population leaving a highly enriched CD4 CD45RA Na ve T Cell population Cells are cryopreserved in Lonza Cryo Media in a cryovial Cell purity is determined post thaw using a FACS assay Count and viability is determined using hemacytometer (trypan blue method) Cells are collected from healthy donors following IRB protocols