Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ HLA B Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ HLA B Cell Enrichment Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-B cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet and without the use of columns. Desired cells are poured off into a new tube and are ready for serology or flow cytometry crossmatch assays.
Abcam Cleaved Caspase-3 Staining Kit (FITC)
Cleaved Caspase-3 Staining Kit (FITC)
The product is subject to the following: Abcam Restricted Use Statement
STEMCELL Technologies RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36, For processing: 200 mL whole blood processed
The RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36 is designed to enrich circulating epithelial tumor cells from fresh whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD16, CD19, CD36, CD38, CD45, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified tumor cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.This cocktail is compatible with small-cell carcinoma samples, including lung cancer samples. If you are working with breast cancer samples, use the RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD56 (Catalog #15137).
American Research Products Inc PrEST Antigen CD14
PrEST Antigen CD14 antigen APrEST83071 for CD14 molecule Recombinant protein fragment of Human CD14
Biotium CD45RA(111-1C5), CF488A conjugate, 0.1mg/mL
Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA (Workshop III). CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4 T-cells, 50% of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO positive) from B cell lymphomas (CD45RA positive).Primary antibodies are available purified, or with a selection of fluoresc
Biolegend MojoSort™ Human CD8 T Cell Isolation Kit
MojoSort™ Human CD8 T Cell Isolation Kit Reactivity: Human; Apps: Cell Sep - Neg; Size: 200 tests
STEMCELL Technologies EasySep™ Human EpCAM Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human EpCAM Positive Selection Kit II is designed to isolate EpCAM+ cells from fresh or previously frozen human mammary epithelial cell preparations by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing EpCAM and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
Miltenyi Biotec, Inc. MACSxpress Buffy Coat Starting Kit
Kit containing MACS Equipment and Reagent for magnetic cell separation directly from buffy coat using MACSxpress Beads
STEMCELL Technologies EasySep™ Mouse CD8a Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD8a Positive Selection Kit II is designed to isolate CD8a+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD8a and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse CD8a Positive Selection Kit (Catalog #18753) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
Miltenyi Biotec, Inc. Monocyte Isolation Kit (BM), mouse
Isolation of untouched mouse monocytes from bone marrow by depletion of non-monocytes
STEMCELL Technologies EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II is designed to isolate CD4+CD25+ T cells from single-cell suspensions of splenocytes or other tissues by negative followed by positive selection. CD4+ T cells are first enriched by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Cocktail. CD25+ cells are then positively selected from the pre-enriched cells using EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail.
STEMCELL Technologies EasySep™ Mouse CD117 (cKIT) Positive Selection Kit, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD117 (cKIT) Positive Selection Kit is designed to isolate CD117+ cells from single-cell suspensions of bone marrow by positive selection. The cocktail contains a combination of PE-labeled antibodies that is directed against CD117, which are then recognized by antibody complexes that are directed against PE and dextran. Labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
STEMCELL Technologies RoboSep™ Human B Cell Enrichment Kit II without CD43 Depletion, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Enrichment Kit II without CD43 Depletion is designed to isolate B cells by negative selection from fresh or previously frozen PBMCs of individuals with B cell leukemia or lymphoma, or other disease states in which B cells may express CD43. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This kit replaces the EasySep™ Human B Cell Enrichment Kit without CD43 (Catalog #19154) for even faster cell isolations.
STEMCELL Technologies EasySep™ Direct Human Basophil Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Basophil Isolation Kit isolates functional, highly purified basophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation, or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.
STEMCELL Technologies EasySep™ Direct Human B-CLL Cell Isolation Kit, For processing: 100 mL whole blood processed
This kit isolates untouched and highly purified B-CLL cells directly from human whole blood by fully immunomagnetic negative selection. The depletion cocktail in this kit does not contain antibodies against CD43, which can be expressed on malignant cells in chronic lymphocytic leukemia (CLL) samples. This cell isolation kit targets non-B cells for removal based on specific surface antigens. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can immediately be used in downstream applications such as nucleic acid extraction, flow cytometry or culture.