Protein Purification Reagents and Kits
Abcam BVT 948, Protein tyrosine phosphatase (PTP) inhibitor, 10MG
MW 241.24 Da, Purity >99%. Non-competitive, irreversible protein tyrosine phosphatase (PTP) inhibitor (IC₅₀ values are 0.9 (PTP1B), 1.7 (TCPTP), 0.09 (SHP-2), 1.5 (LAR) and 0.7 (YopH) μM). Active in vivo and cell-permeable.
The product is subject to the following: Abcam Restricted Use Statement
Biotium CD106 / VCAM1(1.4C3), CF647 conjugate, 0.1mg/mL
CD106 (also known as vascular cell adhesion molecule-1, or VCAM-1) is a member of the Ig superfamily of adhesion molecules. It is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte beta-1 integrin VLA-4 and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
STEMCELL Technologies RosetteSep Human Monocyte Depletion Cocktail, For processing: 200 mL whole blood processed
The RosetteSep Human Monocyte Depletion Cocktail is designed to deplete monocytes from whole blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD36 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep (Catalog 07801) the unwanted cells pellet along with the RBCs. The monocyte depleted fraction is present as a highly enriched population at the interface between the plasma and the buoyant density medium.
Zymo Research Corporation ChIP DNA Clean & Concentrator™ Kit (50 Preps) w/ Zymo-Spin™ I Columns (Uncapped)
The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator™ provides a hassle-free method for the rapid purification and concentration of high quality DNA from any step in a standard ChIP protocol.
Abcam Cyclic Pifithrin-alpha hydrobromide, pifithrin-alpha analog, 10MG
MW 349.3 Da, Purity >98%. Stable, cell-permeable analog of pifithrin-α (ab120478). Inhibits p53-mediated apoptosis and p53-dependent gene transcription. Prevents dexamethasone-induced cell death in murine thymocytes (EC₅₀ = 2.01 μM). Potent STAT6 transcriptional inhibitor. Active in vivo and in vitro. See similar compound
The product is subject to the following: Abcam Restricted Use Statement
Abcam Ciglitazone, PPARgamma agonist, 10MG
MW 333.4 Da, Purity >98%. Selective PPARγ agonist (EC₅₀ = 3 μM). Shows antidiabetic and antineoplastic activity. Induces cell cycle arrest and apoptosis. Active in vivo.
The product is subject to the following: Abcam Restricted Use Statement
STEMCELL Technologies RoboSep Release Mouse CD138 Po, 2 x 10.9 cells
Immunomagnetic positive selection of particle-free mouse CD138 cells
AdipoGen Calreticulin h rec His
Protein. Human calreticulin aa18-417 is fused at the C-terminus to a His-tag. Source E. coli. Endotoxin content <1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl. Purity >90% SDS-PAGE. Calreticulin is involved in regulation of intracellular Ca2 homoeostasis and ER Ca2 capacity. It constitutes together with calnexin and ERp57 the ‘calreticulin/calnexin cycle’ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis.
Zymo Research Corporation EZ DNA Methlyation-Direct™ Kit (50 Rxns)
The EZ DNA Methylation™-Direct™ Kit is a further refinement of our popular EZ DNA Methylation™ and EZ DNA Methylation™-Gold™ kits. These products feature reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of these kits make it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation™-Gold™ kits, DNA denaturation and bisulfite conversion processes are combined into a single step. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
Cayman Chemical MK2 InhIbItr III 500ug
A potent, cell-permeable inhibitor of MK2 (IC50 = 8.5 nM); less potently blocks MK3 and MK5 (IC50s = 210 and 81 nM, respectively) and is weak or inactive against several other kinases, including other p38 MAP kinase targets; prevents LPS-induced synthesis of TNF-α in human monocyte-like U937 cells (IC50 = 4.4 µM)
Biotium Cytokeratin 18(DA7), CF647 conjugate, 0.1mg/mL
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower flu
AdipoGen DNER ED msFc h rec
Protein. Signal peptide and extracellular domain of mouse DNER aa 1-640 are fused at the C-terminus to the Fc portion of human IgG. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS. Purity >85% SDS-PAGE. DNER is an activator of the Notch1 pathway. It mediates neuron-glia interaction during astrocytogenesis.
Aat Bioquest Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
Biotium EGFR(GFR1195 + 31G7), CF647 conjugate, 0.1mg/mL
This MAb recognizes a protein of 170 kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, βcellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.