Protein Purification Reagents and Kits

Protein Purification Reagents and Kits
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Filtered Search Results

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Stationary Phase | Divalent Nickel, Metal Chelate |
---|---|
Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Description | HisPur Ni-NTA Resin |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin |
Product Line | HisPur™ |
Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

Type | Pre-packed Column |
---|---|
Content And Storage | 4°C to 30°C |
Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 2 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ HisPur™ Cobalt Purification Kit, 1 mL
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ HisPur™ Ni-NTA Resin, 10 mL PROMO
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose, 10 mL PROMO
Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL PROMO
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Invitrogen™ Ni-NTA Agarose
Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Type | Agarose |
---|---|
Stationary Phase | Ni-NTA |
Content And Storage | The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored. |
Form | Suspension |
Column Type | Affinity Column |
Cytiva Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Particle Size | 90μm |
---|---|
Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
Flow Rate | 600 cm/hr. |
Height (Metric) | 250mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | Ni Sepharose 6 FF chromatography media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Cross-linked Agarose |
Max. Pressure | 1 bar |
Height (English) | 9.84 in. |
Cytiva StrepTrap XT Chromatography Column
StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

Height (Metric) | 25 mm |
---|---|
Column Type | Affinity Column |
For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™ |
Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Flow Rate | <20 mL/min. |
---|---|
Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Thermo Scientific™ Pierce™ Glutathione Agarose

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Stationary Phase | Glutathione |
---|---|
Type | Agarose |
Content And Storage | Upon receipt store at 4°C. |
Format | Bottle |
Protein Form | Recombinant |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | 40 mg Purified Recombinant GST Protein Binding per mL Resin |
Product Line | Pierce™ |
Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
---|---|
Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
