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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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Keyword Search:
1 – 15 of 5,628 results
1

Thermo Scientific™ HisPur™ Ni-NTA Resin
GSA_VA

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line HisPur
2
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Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
GSA_VA

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

EDGE
Buffer Lysis Buffer
Shipping Condition Ambient
Form Liquid
Product Type Nuclear and Cytoplasmic Extraction Reagent
Reagent Type Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
Product Line NE-PER
3
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Thermo Scientific™ Detergent Removal Spin Columns & Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.

EDGE
Stationary Phase Proprietary Ligand
Column Type Proprietary Resin, Affinity
Purification Target Detergent Removal
4

Thermo Scientific™ Pierce™ High-Capacity EDTA-Compatible Ni-IMAC Resin

Pierce High-Capacity EDTA-Compatible Ni-IMAC Resin is designed for the purification of over-expressed His-tagged proteins from cell culture media.

EDGE
Binding Property Chelator Stable
5

Thermo Scientific™ HisPur™ Cobalt Resin
GSA_VA

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

EDGE
Stationary Phase Metal Chelate, Divalent Cobalt
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Protein Form Recombinant
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Product Line HisPur
6

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose
GSA_VA

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

EDGE
Stationary Phase Divalent Nickel, Metal Chelate
Type Agarose
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Product Line HisPur
7

Cytiva HisTrap™ FF Columns: 5 x 5mL
GSA_VA

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.

EDGE
8
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Cytiva PD MiniTrap™ G-10 Sample Preparation Columns
GSA_VA

Use for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides. Cytiva Life Sciences™ PD MiniTrap™ G-10 Sample Preparation Columns are prepacked, single-use gravity columns.

EDGE
9

Cytiva HisTrap™ HP Prepacked Columns
GSA_VA

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
10

Cytiva HisTrap™ excel Prepacked Columns
GSA_VA

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

EDGE
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 2 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
11

Cytiva™ Protein Select™ Resin

An affinity chromatography resin for purifying recombinant proteins using the self-cleaving Cytiva™ Protein Select™ tag.

12

Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

ENCOMPASS_PREFERRED
Type Agarose
Stationary Phase Ni-NTA
Content And Storage The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.
Form Suspension
Column Type Affinity Column
13

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

14
Promotions Available

Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit
GSA_VA

Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.

15

Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.