Cell Line Lysates
Sino Biological Recombinant Human CCR7/CD197 Protein (VLP, Flag Tag)
A DNA sequence encoding the Human CCR7-VLP (Full Length) (P32248) (Met1-Leu344, thermostabilizing mutations) was fused with a flag tag at the C-terminus. This protein was prepared using our VLP platform.As an innovative technology platform, VLPs are generated through the self-assembly of viral proteins in HEK293 cells. This process efficiently integrates membrane proteins onto the VLP surface, preserving their native conformation and yielding soluble, high-concentration proteins that are ideally suited for immunization and antibody screening.
Zyagen Labs Rat liver tissue lysate is total protein extracted from healthy Sprague Dawley RAT LIVER tissues in lysis buffer contain protease inhibitors. Ideal for many applications including WB & ELISA. Packed at 2mg/ml or 5mg/ml
Zyagen tissue lysate (total protein) is extracted from freshly harvested tissues in protein lysis buffer supplemented with a cocktail of 7 mammalian protease inhibitors to minimize proteolysis. The extracted protein is precisely quantified and packed in 1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if stored properly at -80oC. The quality of protein is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody. The extracted protein is ready for immediate use in reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction.
Zyagen Labs Mouse C57 colon tissue lysate is total protein extracted from healthy C57BL/6 Mouse COLON tissues in lysis buffer contain protease inhibitors. Ideal for many applications including WB & ELISA. Packed at 2mg/ml or 5mg/ml
Zyagen tissue lysate (total protein) is extracted from freshly harvested tissues in protein lysis buffer supplemented with a cocktail of 7 mammalian protease inhibitors to minimize proteolysis. The extracted protein is precisely quantified and packed in 1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if stored properly at -80oC. The quality of protein is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody. The extracted protein is ready for immediate use in reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction.
Cellular Engineering Technologies HepG2 Hepatocellular Carcinoma Expansion Media (450mL)
HepG2 Human Hepatocellular Carcinoma Expansion Media is specifically designed for the robust growth of HepG2 cells. Media is compatible with available animal-origin serum, supplemented separately according to the customer preference.Media is shipped with gel packs.
ABclonal Technology NOMO2 Rabbit pAb
This gene encodes a protein originally thought to be related to the collagenase gene family. This gene is one of three highly similar genes in a region of duplication located on the p arm of chromosome 16. These three genes encode closely related proteins that may have the same function. The protein encoded by one of these genes has been identified as part of a protein complex that participates in the Nodal signaling pathway during vertebrate development. Mutations in ABCC6, which is located nearby, rather than mutations in this gene are associated with pseudoxanthoma elasticum (PXE). Two transcripts encoding different isoforms have been described.
Sino Biological Recombinant Human TRPM7 Protein (GST Tag), Active
Recombinant human CHAK1 (TRPM7) (1158-1865) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Rockland Immunochemicals U-87MG Whole Cell Lysate 500µg
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µL depending on the size format of your gel.
Cayman Chemical 7hydroxy CLOrpromazInhyd 2.5mg
An active metabolite of chlorpromazine; increases plasma prolactin levels in male rats at 5 mg/kg; reverses D-amphetamine-induced depression of the dopaminergic neuronal firing rate in the rat substantia nigra in rats at 1.6 mg/kg
ABclonal Technology TRAF7 Rabbit pAb
Tumor necrosis factor (TNF, see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs, a coiled-coil (leucine zipper) motif, and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.
ABclonal Technology CCR7 Rabbit pAb
The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis. Alternative splicing of this gene results in multiple transcript variants.