Retrovirology Reagents and Kits
Retrovirology Reagents and Kits
631512 \ Pantropic Retroviral Expression System \ Each
The rate at which retroviral vectors bind to and infect cells is mostly controlled by diffusion. During retrovirus infection, only a small fraction of the retroviral vectors can transduce target cells. Virion adsorption is the limiting step of this process. The use of polycations, such as Polybrene, is standard in many retroviral infection protocols owing to the observations of improved infection efficiency. Its mechanism of action is thought to involve neutralization of electrostatic repulsion between virion and cell membranes, enhancing attachment. ViraDuctin™ Retrovirus Transduction Kit is a proprietary formulation for the transduction of retrovirus. After forming a complex with retrovirus in culture medium, the ViraDuctin™ reagents typically provide higher transduction efficiency in many cell types compared to reagents such as Polybrene. It is ideal for transduction of nonpermissive cells such as primary cells and stem cells. 200 transductions.
Lenti-X Packaging Single Shots (VSV-G) provide an extremely simple and consistent one-step method for producing high-titer lentivirus. No additional transfection reagent is needed because Lenti-X Packaging Single Shots (VSV-G) consist of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of VSV-G pseudotyped Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cells (Cat. No. 632180), in a 10 cm dish. 16 Rxns
Quantitatively precipitate any retrovirus with Retro-Concentin™. Instead of using lengthy spins in an ultracentrifuge, Retro-Concentin enables concentration of retroviruses directly from culture medium with a simple one (or an optional two) low-speed centrifugation steps. In addition, Retro-Concentin stabilizes the retroviruses for frozen storage, providing an additional advantage over other concentration methods. Each preparation can handle up to 200 mL of retroviral supernatant, which is compatible with most centrifuges, and the resulting pellet can be dissolved in the volume of buffer that meets your experimental requirements.
101 BIO Retrovirus 10X Titer-Up Reagent (Increase virus titer by 10 times) | 1 mL for packaging 500 mL retrovirus soup
This retrovirus titer boost is a novel recipe of small molecules designed for increasing / boosting retrovirus packaging efficiency. Retrovirus 10X Titer-Up stably regulates the viral RNA packaging on the transcriptional level, which can enhance production of retroviral particles for up to 10 fold.
pCMV-VSV-G Envelope Vector can be used in co-transfections with other packaging plasmids to make recombinant lentivirus or retrovirus.
Clone your gene of interest into Cell Biolabs' pBABE-puro Retroviral Vector and then package into a retroviral particle using a retroviral packaging cell or co-transfect with retroviral packaging constructs into 293T cells.
The KILR Retroparticles for Suspension Cells (G418) are MMLV particles designed to transduce suspension cells. This allows production of ProLabel-tagged, G418-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 4 vials of cryopreserved retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-suspension-cells-(g418)-97-0002.
BD Retro-X Universal Packaging System, 8 RXN, Use w/any Retroviral Vector, 100% Cell Transduce, Efficiently Transduce Hard-To-Transfect Cell Lines, Easily Create Stable Cell Lines, Preparation and Storage: -196degreeC