DNA Polymerases
Enzo Life Sciences AMPIGENE Taq Mix (40Reactions)
AMPIGENE Taq Mix uses the latest developments in polymerase technology and buffer chemistry to optimize PCR. The AMPIGENE Taq Mix is a robust mix for routine PCR applications including genotyping, screening, and library construction. An optimized buffer system allows efficient amplification under fast and standard cycling conditions. Applications: PCR.
Genscript Corporation iTaq/i DNA Polymerase con
i Taq /i DNA Polymerase is a thermostable DNA polymerase isolated from an i E coli /i strain that carries the i Taq /i DNA polymerase gene i Taq /i DNA Polymerase is the most common polymerase used in PCR In some cases such as RAPD PCR adding large volume of general i Taq /i DNA polymerase (5 U/ l) which has a high concentration of glycerol in its storage buffer will increase the glycerol concentration in the reaction mix interfering with PCR performance The use of concentrated i Taq /i DNA Polymerase (25 U/ l ) with a far slimmer dose of glycerol can prevent poor PCR efficiency br br b Note /b Concentrated i Taq /i DNA Polymerase (GenScript E00012) is supplied with 10X reaction buffer containing 15 mM magnesium chloride The a href https //www genscript com/enzyme/D0056-dNTP mixture 10 mM each html dNTP (10 mM) mix /a may be ordered separately
Zymo Research Corporation ZymoTaq™ Polymerase (50 Rxns)
The inclusion of a heat-activated, thermal-stable DNA polymerase reduces primer dimer and non-specific product formation that can occur when performing conventional PCR. This unique product is specifically designed for the amplification of bisulfite-treated DNA for methylation detection, real-time and quantitative PCR that are SYBR Green and probe based. The product generates specific amplicons with little or no by-product formation. Simple and easy to use: heat at 95 C for 10 minutes to initiate polymerization.ZymoTaq™ DNA polymerase is a heat-activated, hot startpolymerase that has 3-terminal transferase activity. Theaddition of A overhangs to amplified DNA makes it ideal for use in TA-cloning.
Alkali Scientific Radiant™ HiFi Ultra Polymerase 1000 Reactions 5 x 100μL, 2u/μL HiFi Ultra Polymerase & 15 x 1mL 5x HiFi ultra Buffer (includes dNTP’s)
Ultra-High Fidelity Including dNTP's RadiantTM HiFi Ultra DNA Polymerase is a high-performance, high-fidelity DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA. RadiantTM HiFi Ultra Polymerase possesses 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities with an error-rate of 4.55 x 10-7 . The polymerase is ideal for cloning applications, library construction, screening and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM HiFi Ultra Polymerase is engineered for robust, superior high-fidelity PCR in comparison with standard proof-reading enzymes such as pfu DNA Polymerase. Several point-mutations combined with a new-generation buffer formulation provide for significantly improved processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples. The supplied 5X HiFi Ultra Reaction Buffer contains an ideal concentration of MgCl2 and
Medchemexpress LLC T4 DNA Polymerase T 10ug | 10ug
T4 DNA Polymerase a pivotal enzyme in viral DNA replication performs dual functions crucial for genome synthesis As a polymerase it synthesizes DNA and with its exonucleolytic activity it proofreads by degrading single-stranded DNA in the 3 - to 5 -direction ensuring the accuracy and fidelity of replicated viral genomic DNA T4 DNA Polymerase Protein T4 phage (His) is the recombinant T4 DNA Polymerase protein expressed by E coli with N-6 His labeled tag T4 DNA Polymerase Protein T4 phage (His) has molecular weight of 103 6 kDa
INTACT GENOMICS INC T4 UvsX DNA Recombinase, Glycerol Free 1000µg
Glycerol Free T4 UvsX DNA Recombinase is useful for Recombinase Polymerase Amplification (RPA) and other applications.Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. T4 UvsX DNA Recombinase and other recombinases form presynaptic filaments on ssDNA that are activated to search for homology in dsDNA and to perform DNA strand exchange.
INTACT GENOMICS INC Sau DNA Polymerase Large Fragment 1000 Units (5 units/µl)
Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.Applications Strand displacement DNA synthesis (1) Random primer labeling Second strand cDNA synthesis dA-tailing
Bioworld Firepol (Thermostable TAQ), 1000 Unit(s)
A thermostable DNA polymerase FirePol is similar to Taq DNA polymerase I (homology 98%). 3' to 5' exonuclease activity. Has the "extendase activity", allowing TA cloning. Due to the unique modifications and purification technology it is very thermostable at high temperatures. Can be used in all applications involving polymerase chain reaction (PCR). Contains necessary reaction buffers and MgCl2.
Bioworld Firepol (Thermostable TAQ), 4000 Unit(s)
A thermostable DNA polymerase FirePol is similar to Taq DNA polymerase I (homology 98%). 3' to 5' exonuclease activity. Has the "extendase activity", allowing TA cloning. Due to the unique modifications and purification technology it is very thermostable at high temperatures. Can be used in all applications involving polymerase chain reaction (PCR). Contains necessary reaction buffers and MgCl2.
ABclonal Technology Heat-T4 DNA Ligase
Heat-T4 DNA Ligase is a thermostable mutant of T4 DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5? phosphate and 3? hydroxyl termini in duplex DNA or RNA. It is designed to function at higher temperatures than wild-type T4 DNA ligase. The catalytic reaction requires ATP as a cofactor. Heat-T4 DNA Ligase will join blunt end and cohesive end termini at temperatures as high as 45?C , and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. At the same time, T4 DNA ligase closes the gaps in these DNA substrates.
ABclonal Technology T4 DNA Polymerase (5,000 U/mL)
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5to3 direction and requires the presence of template and primer. This enzyme has a 3to5 exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5to3 exonuclease function. It is applicable to 3 overhang removal to form blunt ends, 5 overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.
CHIMERX Human DNA Polymerase Alpha, 200 u
Exclusively from CHIMERx, Human DNA Polymerase Alpha is a replication enzyme used to test the toxicity of drugs on key human replication systems.DescriptionThe simplest DNA Polymerase known in both size and catalysisReplication enzyme involved in initiation of DNA replication in eukaryotesA multi-subunit complex, which inhibits DNA polymerase and primase activitiesIsolated from HeLa cells by immunoaffinity chromatography (1)ApplicationsUsed to test in vitro replication capabilities along with other enzymesUsed to test the toxicity of drugs on key human replication systemsUsed in DNA replication experimentsUnit DefinitionOne unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C
Agilent Technologies PFUTURBO DNA POLYMERASE
PfuTurbo DNA Polymerase 1000 U Robust high-fidelity enzyme for PCR cloning with enhanced PCR product yield and length capabilities Contains: PfuTurbo DNA polymerase 10X cloned Pfu buf