DNA Polymerases
Genscript Corporation Taq DNA Polymerase without Mg
DNA Polymerase is a thermostable DNA polymerase isolated from an i E coli /i strain that carries the i Taq /i DNA polymerase gene i Taq /i DNA polymerase is the most common polymerase used for PCR
New England Biolabs, Inc. Bst 2.0 WarmStart DNA Polymerase – 8000 units
Bst 2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45C. The aptamer rapidly releases the Bst 2.0 WarmStart DNA Polymerase above 45C and therefore no special activation step is needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5' to 3' DNA polymerase activity and strong strand-displacement activity but lacks 5' to 3' exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
Alkali Scientific Radiant™ Taq DNA Polymerase, 5u/µl, 10,000 units w/ separate tube 10X PCR buffer and MgCl2
Radiant™ Taq DNA Polymerase Description RadiantTM Taq DNA Polymerase is a highly purified, high performance DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA. RadiantTM Taq DNA Polymerase exhibits 5´-3´ DNA polymerase activity with an error-rate of wild-type Taq ( 2.0 x 10-5). The polymerase is ideal for screening, colony PCR, high-throughput PCR and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM Taq DNA Polymerase is engineered for robust, superior PCR and is supplied with a highly optimized, new-generation buffer system which provides exceptional sensitivity. Storage RadiantTM Taq DNA Polymerase is shipped on blue or dry ice and should be stored at –20°C upon receipt. Excessive freeze/thawing should be avoided. When stored as specified, RadiantTM Taq DNA Polymerase is stable for 12 months from date of receipt. The Kit may also be stored at 4°C for 1 month. Important Cons
ILEX LIFE SCIENCES LLC Taq DNA Polymerase Recombinant (10000 U)
Taq DNA Polymerase Recombinant is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74¿C and exhibits a half-life of 40 minutes at 95¿C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5¿~3¿ direction in the presence of magnesium and also possesses a 5¿~3¿ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions. BUFFERS: Includes Storage Buffer, 10X Reaction Buffer with MgCl2, and 10X Reaction Buffer without MgCl2 and separate MgCl2. SOURCE: Recombinantly produced in E. coli. FORMULATION: Taq DNA Polymerase solution in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20. PURITY: Greater than 95.0% as determined by SDS-PAGE.