Substrates
Sino Biological Peptide Substrates: Histone H3 Peptide (1-21)
The Histone H3 Peptide (1-21) sequence (ARTKQTARKS-TGGKAPRKQLAGGKKC) is derived from human histone H3 (1-21) and is suitable for use as the substrate for histone methyltransferase (at K4 and K9) and acetyltransferase (at K9 and K14) family assays.
Neogen Diagnostics K-Gold PNPP Substrate, 500 mL, Microwell, AP, Colorimetric
K-Gold Substrate is a one-bottle stabilized chromogenic substrate for use with alkaline phosphatase based immunoassays. K-Gold Substrate utilizes the substrate p-nitrophenyl-phosphate (PNPP), which reacts with alkaline phosphatase to produce the chromophore p-nitrophenol. NEOGENs ready-to-use K-Gold formula does not require mixing of additional ingredients or stabilizing agents, making it an ideal formula for kit manufacturers. Stable for a minimum of 30-months when stored at 2-8C.
Medchemexpress LLC Ac-Trp-Leu-Ala-AMC | 1104011-59-7 | 99.8% | 587.7 g/mol | C32H37N5O6 | 10MG
Ac-WLA-AMC (Ac-Trp-Leu-Ala-AMC; CAS 1104011-59-7) is an acetylated tripeptidyl fluorogenic substrate for measuring the chymotrypsin-like activity of the 20S proteasome β5 (β5c) subunit. Enzymatic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal used in proteasome activity assays and inhibitor screening.
- Measures chymotrypsin-like proteasome β5 activity.
- Releases 7-amino-4-methylcoumarin (AMC) for sensitive fluorescence detection.
- Compatible with kinetic and end-point fluorescence assays.
- Supplied as a solid peptide substrate suitable for biochemical assays.
- High purity (99.8%) appropriate for research use.
Abcam Carbo x yl-DCFDA (5(6)-Carbo x y-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate, 1G
MW 529.28 Da, Purity >85%. Amine-reactive, fluorogenic, non-selective esterase substrate. Hydrolyses to DFCA (ab145439) fluorophore intracellularly. Labels intact cells. 2,7-Dichlorofluorescein (ab146332) derivative and DCFDA N-succinimidyl ester (ab145286) precursor.
The product is subject to the following: Abcam Restricted Use Statement
Ubpbio K63-linked di-Ubiquitin (human), enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
Sino Biological Peptide Substrates: IRS1 (Y608) Peptide
The synthetic IRS1 (Y608) Peptide [KKHTDDGYMPMSPGVA] is derived from mouse insulin receptor substrate 1 (amino acids 603-616) and is suitable as substrate for JAK1 and JAK2.
Sigma Aldrich Fine Chemicals Biosciences Albumin–fluorescein isothiocyanate conjugate, MFCD00282182, 100 mg
Protein bovine, Synonym: FITC-albumin. Albumin−fluorescein isothiocyanate conjugate has been used: as a control to compare the intracellular delivery efficiency of proteins with or without carrier, for the fluorescent visualization of proteins and cells, as a blocking agent in ELISA, as a chemical for brain perfusion and fixation, for in vivo capillary–epithelial permeability experiment, and for confocal microscopy of right ventricular (RV) vasculature in rats.
Surmodics Inc BCIP/NBT Purple One Component AP Membrane Substrate, 1L
The substrate is a ready to use, one-component formulation of 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium for use in immunoblotting or other membrane applications.
Enzo Life Sciences OMNIMMP fluorogenic substrate (1mg)
Fluorogenic substrate for nearly all matrix metalloproteinase (MMP) enzymes, and ADAM17/TACE. This highly-quenched (99.5%) substrate is useful for inhibitor screening and kinetic analysis. Ex.: 328 nm, Em.: 393 nm, although the following Ex/Em have been used: 320-340/400-420. Also available: OMNIMMP Fluorogenic Control Peptide (BML-P127). Alternative name: Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 . AcOH, Matrix metalloproteinase substrate. Purity: ≥98%. Solubility: Soluble in methanol (1mg/ml) or DMSO (20mg/ml). Long Term Storage: -20°C. Applications: WB Assay.
CHEMPEP INC Ubiquitin-AMC, 100 μg, Fluorogenic substrate for DUB activity assays, C388H636N106O119S, Molecular Weight 8722.2 g/mol, Fluorescence Excitation 380 nm, Emission 460 nm
Ubiquitin-AMC is a highly sensitive fluorogenic substrate essential for measuring deubiquitinating enzyme (DUB) activity in biochemical assays. This versatile reagent, composed of ubiquitin conjugated to the AMC fluorophore, facilitates real-time monitoring of DUB activity by releasing a fluorescent signal. It is particularly advantageous for high-throughput screening and detailed kinetic studies in various cellular processes.
- 100 μg quantity
- Molecular formula: C388H636N106O119S
- Molecular weight: 8722.2 g/mol
- Fluorogenic substrate type
- Target enzyme class: Deubiquitinating enzymes (DUBs)
- Real-time monitoring capability
- High sensitivity for biochemical research
- Uses aqueous buffers for solubility
- Storage temperature: -20°C or below
- Avoid repeated freeze-thaw cycles
- Purity of ≥95%
- Synthetic source of ubiquitin
- Supplied as lyophilized powder or solution in DMSO
Medchemexpress LLC H-Asp(AMC)-OH | 133628-73-6 | MFCD00236804 | 99.8% | 290.27 g/mol | C14H14N2O5 | 10 MG
H-Asp(AMC)-OH is an aspartic acid derivative linked to 7-amino-4-methylcoumarin (AMC) used as a fluorescent substrate in biochemical and enzymatic assays. It is supplied in small research quantities and characterized for assay use with reported high purity and defined storage recommendations.
- Fluorescent aspartic acid derivative for use as a substrate in assays.
- Does not inhibit glycine transport at a concentration of 0.25 mM.
- High purity (99.75%) suitable for research applications.
- Molecular weight 290.27 g/mol; formula C14H14N2O5.
- Available in a 10 mg pack size for laboratory use.
- Store sealed, away from moisture and light; in solvent: -80°C for 6 months, -20°C for 1 month.
Medchemexpress LLC Z-leu-leu-glu-AMC | 348086-66-8 | MFCD01074989 | 99.5% | 664.75 g/mol | C35H44N4O9 | 1MG
Z-Leu-Leu-Glu-AMC is a fluorogenic tripeptide substrate used to measure post-acidic (caspase-like) hydrolytic activity of the 20S proteasome. Proteolytic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal suitable for kinetic assays, inhibitor screening, and proteasome activity profiling in biochemical research.
- High purity (≈99.5%) for reliable assay performance.
- Releases AMC for sensitive fluorometric detection.
- Suitable for 20S/26S proteasome activity and inhibitor assays.
- Available as solid and 10 mM DMSO solution formats.
- Supplied in small research pack sizes for convenient use.
Eurofins Discoverx Coelenterazine h, 1 mg. (90-0084)
This product is an optimized form of coelenterazine substrate that is significantly more sensitive to calcium, intended for use in aequorin-based luminescent calcium assays. This product is shipped as a dry powder. This product contains 1 mg of dry powder. For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
![Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 10 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660224.jpg-250.jpg)
Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 10 Grams
Luciferin Sodium Salt (10 g) is a luminescent substrate widely used in cell assays for detecting biological activity through light emission. This reagent is ideal for studying ATP levels, enzyme activities, and other cellular processes that result in light emission upon reaction with luciferase. The 10 g formulation is designed for large-scale experiments and is commonly used in bioluminescence assays, including cell viability testing, gene expression analysis, and bioassay development. The luminescent properties of luciferin provide researchers with a sensitive and accurate method for detecting cellular processes, making it an essential tool in pharmacology, biochemistry, and molecular biology.
![eMolecules 115144-35-9 | D-Luciferin potassium | Ambeed | MFCD00044928 | 318.410 | C11H7KN2O3S2 | 97.000 | [K+].Oc1ccc2nc(sc2c1)C1=N[C@H](CS1)C([O-])=O | 1g | 525077369](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000482816.jpg-250.jpg)
eMolecules 115144-35-9 | D-Luciferin potassium | Ambeed | MFCD00044928 | 318.410 | C11H7KN2O3S2 | 97.000 | [K+].Oc1ccc2nc(sc2c1)C1=N[C@H](CS1)C([O-])=O | 1g | 525077369
D-Luciferin potassium | Ambeed | 115144-35-9 | MFCD00044928 | 318.410 | C11H7KN2O3S2 | 97.000 | [K+].Oc1ccc2nc(sc2c1)C1=N[C@H](CS1)C([O-])=O | 1g | 525077369