Substrates
Sigma Aldrich Fine Chemicals Biosciences N-Succinyl-Gly-Gly-Phe-p-nitroanilide protease substrate | 68982-90-1 | MFCD00038721 | 10MG
N-Succinyl-Gly-Gly-Phe-p-nitroanilide protease substrate | Purity: >=98% (TLC) | Mol Wt: 499.47 | 68982-90-1 | MFCD00038721 | 10MG
Enzo Life Sciences Arachidonoyl-AMC (5 mg)
Fluorogenic substrate for fatty acid amide hydrolase (FAAH). This substrate is the basis for a simple, selective and sensitive assay for FAAH which is amenable to high-throughput screening. Purity: ≥99%. Solubility: Soluble in DMSO (>25mg/ml). Long Term Storage: -80°C.
Revvity Health Sciences Inc HTRF Biotin-APP-Eu cryptate (BACE1 substrate), 20,000 Assay Points
HTRF Biotin-APP-Eu cryptate (BACE1 substrate), 20,000 Assay Points
Medchemexpress LLC Z-Gly-Gly-Arg-AMC acetate | 2070009-61-7 | 99.84% | 639.66 | 50 MG
Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate primarily used for testing thrombin generation in both platelet-rich plasma (PRP) and platelet-poor plasma (PPP). This compound, characterized by excitation/emission wavelengths of 390/480 nm, is provided as a solid, typically white to off-white, and is strictly for research applications.
- Thrombin-specific fluorogenic substrate
- Suitable for thrombin generation assays in PRP and PPP
- Exhibits excitation at 390 nm and emission at 480 nm
- Supplied as a white to off-white solid
- Intended for research use only
Selleck Chemical LLC D-Luciferin
D-Luciferin is a popular bioluminescent substrate of luciferase in the presence of ATP used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications In an immunocompetent mouse model of ovarian cancer the use of D-luciferin substrate and firefly luciferase preserves tumour-host immune interactions since bioluminescent imaging is a more sensitive indication of tumour growth than weight gain
Selleck Chemical LLC D-Luciferin
D-Luciferin is a popular bioluminescent substrate of luciferase in the presence of ATP used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications In an immunocompetent mouse model of ovarian cancer the use of D-luciferin substrate and firefly luciferase preserves tumour-host immune interactions since bioluminescent imaging is a more sensitive indication of tumour growth than weight gain
Medchemexpress LLC N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin | 169332-61-0 | 99.2% | 675.64 | 25 MG
Ac-DEVD-AMC is a fluorescent substrate for caspase-3 and caspase-7. Upon treatment with cell lysate, it facilitates the release of amino-4-methylcoumarin (AMC), which allows for fluorescence detection at an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
- Fluorescent substrate of caspase-3/caspase-7
- Releases amino-4-methylcoumarin (AMC) for fluorescence detection
- Excitation wavelength of 380 nm
- Emission wavelength of 460 nm
Enzo Life Sciences Chk 1 & 2 substrate (1mg)
This peptide sequence, KKKVSRSGLYRSPSMPENLNRPR, corresponds to amino acids 206-225 of the phosphatase CDC25C, which is a phosphorylation target of Chk1 on Ser216. The addition of three N-terminal lysines allows the peptide to be used in phosphocellulose kinase assays. Both Chk1 and Chk2 will phosphorylate this peptide. Alternative name: CDC25C (206-225). Long Term Storage: -20°C.
Enzo Life Sciences FLUOR DE LYS-Green substrate (50µl)
The FLUOR DE LYS-Green substrate offers a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has two steps. First, the FLUOR DE LYS-Green Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS Developer (BML-KI105) produces a fluorophore. The FLUOR DE LYS-Green substrate has a higher wavelength excitation and emission than the original FLUOR DE LYS substrate (BML-KI104). This avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue range. Formulation: 50mM solution in DMSO. Purity: ≥95% (HPLC). Long Term Storage: -80°C.
Sigma Aldrich Fine Chemicals Biosciences Succinyl coenzyme A sodium salt | 108347-97-3 | MFCD00057770 | 25 mg
Succinyl coenzyme A sodium salt | Purity: ≥85% | Mol Wt: 867.61 | 108347-97-3 | MFCD00057770 | 25 mg
Enzo Life Sciences Z-YVAD-AFC (5mg). CAS: 201608-13-1
Fluorogenic substrate for caspase-1. AFC has an excitation maximum of 400nm and an emission maximum of 505nm. This product is similar to Ac-YVAD-AMC, ALX-260-024, but the AFC fluorophore has a greater Stokes' shift upon cleavage and the N-terminal is blocked by a benzyloxycarbonyl moiety, which increases cell permeability. Alternative name: Caspase 1 substrate (fluorogenic). Formula: C39H40N5O11F3. MW: 811.8. Purity: ≥95% (HPLC). Single spot (TLC). Solubility: Soluble in DMSO, DMF or methanol; insoluble in water. Long Term Storage: -20°C.
Enzo Life Sciences TACE substrate (fluorogenic) (0.5mg). CAS: 192723-42-5
Fluorogenic substrate for TACE (ADAM17) (between Ala-Val), as well as MMP-3, MMP-7, MMP-12, and MMP-17, but not for ADAM19, MMP-13, nor MMP-14. Similar substrates are also cleaved by ADAM10, ADAM8 and ADAM9, and MMP-1, -2, and -9. This quenched substrate is useful for TACE inhibitor screening and kinetic analysis. Ex: 328 nm, Em: 393 nm, although the following Ex/Em have been used: 320-340/400-420. Also available: OMNIMMP Fluorogenic Control peptide (BML-P127). Alternative name: ADAM17 substrate. Purity: ≥96%. Long Term Storage: -20°C.