Substrates
Bachem H-Ala-Ala-Ala-OH
H-Ala-Ala-Ala-OH, 5874-90-8, C9H17N3O4, Mr 231.25, 1g. Trialanine. Substrate for dipeptidyl peptidase II (DPP II).
Bioworld BCIP, 1 g
BCIP Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue, insoluble product. This product then reacts to form a chromogen homodimer. This reaction may be used to amplify the signal by catalyzing the reduction of a tetrazolium salt (usually NBT) to form an intenselycolored, insoluble formazan chromogen. Technical Information:Ultra Pure GradePurity (enzymatic): >99.0%Purity (UV): >99.0%Inhibitors (enzymatic): <0.1%Solubility (0.1% phosphate buffer): Clear and haze-freeSolubility (0.10% DMF): Pass (Clear/Haze-free)Melting Point: 195-197CA260 (1mg/ml, DMF): 0.010Ref: Unger, et al., Am. J. Surg. Pathol., 10(1): 1-8, 1986.
Apexbio Technology LLC D-Luciferin (potassium salt) 115144-35-9 100mg
D-Luciferin (potassium salt) a potassium salt derivative of D-luciferin serves as the bioluminescent substrate for firefly luciferase The enzyme-mediated oxidation of D-luciferin in the presence of ATP and oxygen produces bioluminescent emission at approximately 560 nm without the need for external excitation This property is applied extensively in bioluminescence imaging (BLI) experiments including live-cell and small-animal imaging Cells genetically engineered to express luciferase utilize D-luciferin to generate optical signals facilitating non-invasive visualization and tracking of biological events such as tumor growth gene expression dynamics in vivo distribution and therapeutic responses
Biotium Coelenterazine FCP
Coelenterazine fcp is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is 135 times higher than that of aequorin formed from native coelenterazine. Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator. It binds coelenterazine to form an aequorin complex that emits light upon calcium binding and measures concentrations ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter of transcription regulation and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines are also used for chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues. Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines are also available.Properties : Yellow solid soluble in MeOH or EtOH; Store at -20°C, protect from light; C25H24FN3O2; MW: 417.48.
Research Products International Corp BCIP, P-Toluidine Salt, 1 G
- Substance Name: 5-Bromo-4-Chloro-3-Indolyl-Phospate-P-Toluidine Salt
- CAS Number: 6578-06-9
- Molecular Formula: C8H5BrCINO4P • C7H10N
- Molecular Weight: 433.6
Biotium RHODAMINE 110 BZIPAR 5mg
BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin. The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases.Properties: Ex/Em of end product (R110) = 496/520 nm; Off-white to pink solid soluble in DMSO; Store at 4°C and protect from light; C70 H88Cl2N14O13; MW: 1404.5
Biotium Coelenterazine (ALSO KNOWN AS NATIVE COE
Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator whereas Renilla luciferase is commonly used as a reporter of transcription regulation. Coelenterazine binds apoaequorin to form an aequorin complex which emits light upon calcium binding, and can measure a broad range of calcium concentrations from ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter protein and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines can also be used for chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.Coelenterazine (native): Yellow solid soluble in MeOH or EtOH; Store at -20°C and protect from light; C26H21N3O3; MW: 423; [55779-48-1]. See also other Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines (native and analog h) for in vivo use.
Sino Biological Peptide Substrates: ZIPtide
The ZIPtide substrate peptide sequence (KKLNRTLSFAEPG) is routinely evaluated as a substrate for DAPK3 (ZIPK).
Sino Biological Peptide Substrates: PAKtide
The 10 amino acids of PAKtide peptide (RRRLSFAEPG) contain a serine/threonine protein kinase phosphorylation site in a common seven-residue epitope (1, 2).
Sino Biological Peptide Substrates: PP2B Substrate
The PP2B Substrate peptide [DLDVPIPGRFDRRV(p)SVAAE] is derived from bovine PRKAR2A (cAMP-dependent protein kinase type II-alpha regulatory subunit) (amino acids 82-100) and is suitable as PP2B substrate.
Sino Biological Peptide Substrates: CATCHtide
The CATCHtide peptide sequence (CRRHYYYDTHTNTYY-LRTFGHNTRR) is derived from human SLC12A2 (amino acids 198-217) and is suitable as a substrate for kinases OSR1 and STK39.
Medchemexpress LLC D-luciferin (potassium) | 115144-35-9 | 99.1% | 318.41 g/mol | C11H7KN2O3S2 | 500MG
D-Luciferin potassium is the potassium salt of D-luciferin, the native substrate for firefly luciferase. It is provided as a water-soluble reagent for use in in vitro and in vivo bioluminescence assays and imaging, intended to deliver consistent luminescent signals with appropriate storage for stability.
- Water-soluble potassium salt form suitable for aqueous assays.
- Natural substrate for luciferase-based bioluminescent detection.
- High purity (99.1% by LCMS) for reproducible assay results.
- Molecular weight 318.41 g/mol enables precise dosing.
- Stable when stored sealed at 4°C; in solvent stable at -80°C for up to 2 years.
- Suitable for reporter assays and in vivo bioluminescence imaging.