Substrates
TARGETMOL CHEMICALS INC D-Luciferin 100MG
Also available in 10 mg, 50 mg, 200 mg, 500 mg and bulk. Please contact Fisher for quotes. D-Luciferin (D-(-)-Luciferin) is a luciferase (Luc) substrate that catalyzes the production of typical yellow-green light (560 nm) by bioluminescent insects. D-Luciferin can be used in luciferase assays. Purity 99.83%
Abcam AFC (7-Amino-4-trifluoromethylcoumarin), Fluorescent marker
Fluorescent marker. Substrate for fluorogenic enzyme assays.
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Boc-Leu-Gly-Arg-AMC | 65147-09-3 | 98.3% | 601.69 | 5 MG
Boc-Leu-Gly-Arg-AMC is a fluorogenic AMC substrate primarily used for convertases. This compound is suitable for use in enzymatic assays. Research has shown its application in developing enzymatic assays to detect proteolytic enzymes, such as those capable of generating sodefrin, by measuring the liberation of 7-amino-4-methylcoumarin. The optimal activity for cleaving Boc-Leu-Gly-Arg-AMC in certain extracts has been observed at pH 9.0 and 45 °C.
- Fluorogenic AMC substrate for convertases.
- Can be used in enzymatic assays.
- Suitable for demonstrating the presence of proteolytic enzymes.
Enzo Life Sciences AEC peroxidase substrate kit (300ml)
The AEC Peroxidase Substrate Kit contains reagents for visualization of peroxidase reactions using Aminoethylcarbazole (AEC). In the presence of H2O2, peroxidases catalyze the oxidation of AEC into a brick red precipitate. The supplied reagents are sufficient for preparation of 300 ml of ready-to-use color reaction mixture. Applications: Colorimetric detection .
Enzo Life Sciences Bio-16-ddUTP (25nmol)
Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labeling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labeled oligonucleotides produced by this reaction are suitable as hybridization probes. Biotin-labeled cDNA produced by this reaction can be used in nucleic acid array assays. Biotin-labeled DNA in hybridization assays can be detected by a reporter molecule linked to streptavidin, avidin, or and anti-biotin antibody. Such a complex can be detected directly, e.g. by the excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate. Alternative name: Biotin-16-2',3'-dideoxyuridine-5'-triphosphate . 4Li. Concentration: 1mM. Purity: ≥93% (HPLC). Formulation: Liquid. Solution in water.
Medchemexpress LLC Ac-Leu-Arg-AMC | 929621-79-4 | 98.2% | 486.56 | 5 MG
Ac-Leu-Arg-AMC is a fluorogenic peptide substrate used in various research applications. It is supplied as a solid with a purity of 98.2% and a molecular weight of 486.56. This compound is known for its fluorescent properties with an excitation wavelength of 390 nm and emission at 480 nm, making it suitable for fluorescence-based assays.
- Fluorogenic peptide substrate
- White to off-white solid appearance
- Excitation (Ex) at 390 nm
- Emission (Em) at 480 nm
- Soluble in DMSO (300 mg/mL)
Enzo Life Sciences Ac-YVAD-2,6-dimethylbenzoyloxymethylketone (5mg)
Irreversible, cell permeable inhibitor of caspase-1. Also inhibits caspase-4. Alternative name: Caspase 1 inhibitor, Ac-Tyr-Val-Ala-Asp-2,6-dimethylbenzoyloxymethylketone. Formula: C33H42N4O10. MW: 654.7. Purity: ≥95% (HPLC). Appearance: White powder. Peptide content: 78-99%. Solubility: Soluble in DMSO or methanol. Long Term Storage: -20°C. Use/Stability: Stock solutions should be divided into aliquots and stored at -20°C. Handling: Keep cool and dry.
Enzo Life Sciences Ac-WEHD-AMC (5mg)
Fluorogenic substrate for caspase-1 (Km=4µM), -4 (Km=31µM);and -5 (Km=15µM). This fluorogenic caspase-1 substrate with an optimal tetrapeptide sequence is cleaved 50-fold more efficiently than Ac-YVAD-AMC (ALX-260-024). Alternative name: Caspase 1 substrate (fluorogenic), Caspase 5 substrate (fluorogenic), Caspase 4 substrate (fluorogenic). Formula: C38H40N8O11. MW: 784.8. Purity: ≥98% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO (100mM, 75.5mg/ml). Dilute with distilled water or buffer. Long Term Storage: -20°C.
Enzo Life Sciences Ac-YVAD-CHO (1mg)
Inhibitor of caspase-1 and -4. Potent and reversible inhibitor of caspase-1. Also inhibits caspase-4. Strongly inhibits anti-Fas induced apoptosis in L929-Fas cells. Alternative name: Caspase-1 inhibitor (aldehyde). Formula: C23H32N4O8. MW: 492.5. Purity: ≥96% (HPLC). Appearance: White powder. Formulation: Lyophilized. Peptide content: 70-90%. Solubility: Soluble in DMSO or water (both 50mg/ml). Long Term Storage: -20°C. Use/Stability: Stock solutions should be divided into aliquots and stored at -20°C. Handling: Keep under inert gas. Keep cool and dry.
Enzo Life Sciences Ac-YVAD-AMC (5mg)
Fluorogenic caspase-1 substrate with a Km=14µM. Ex.:340-360nm, Em.:440-460nm.Sequence is based on precursor interleukin-1β Asp116 cleavage site for caspase-1. Alternative name: Caspase-1 substrate (fluorogenic). Formula: C33H39N5O10. MW: 665.7. Purity: ≥98% (HPLC). Appearance: White to off-white powder. Peptide content: 75-95%. Solubility: Soluble in DMSO. Dilute with distilled water or buffer, pH 7.0-7.5 (e. g. TRIS, HEPES). Long Term Storage: -20°C. Handling: Protect from light. Keep cool and dry.
Enzo Life Sciences Ac-WEHD-pNA (5mg)
Colorimetric caspase substrate. Combinatorial studies have found the WEHD sequence to be optimal for clevage of tetrapeptide substrate by caspase-1, caspase-4, caspase-5 and caspase-14. Cleavage of the substrate by a caspase releases pNA, which can be detected by its absorbance at 405nm. Alternative name: Caspase 1 substrate (chromogenic), Caspase 5 substrate (chromogenic). Formula: C34H37N9O11. MW: 747.7. Purity: ≥98% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO. Dilute with buffer, pH 7.5. Long Term Storage: -20°C.
Medchemexpress LLC ATR kinase substrate peptide | 1232497-16-3 | 99.30% | 1814.05 | 5 MG
ATR kinase substrate peptide (ASELPASQPQPFSAKKK) is a peptide substrate for ATR protein kinase and can be used to detect ATR kinase activity. This product is for research use only.
- Sequence: Ala-Ser-Glu-Leu-Pro-Ala-Ser-Gln-Pro-Gln-Pro-Phe-Ser-Ala-Lys-Lys-Lys
- Target: ATR (Ki 6 nM)
- Solubility in H2O: 28 mg/mL (15.44 mM; requires ultrasonic and warming)
- Appearance: Solid, white to off-white
Revvity Health Sciences Inc LANCE Ultra ULight Anti-GST Antibody, 10nmoles
LANCE Ultra ULight Anti-GST Antibody, 10nmoles
