Substrates
Enzo Life Sciences WP-AMC (5mg)
Fluorogenic substrate likely cleaved by dipeptidyl peptidase IV (DPIV, DPPIV, CD26), DPP8, and other prolyl peptidases. Can be used in vitro, for inhibitor screening, and in biological fluids. Ex = 380 nm, Em = 460 nm. Alternative name: Trp-Pro-AMC. Purity: ≥95% (HPLC). Formulation: Lyophilized. Solubility: Soluble in water or DMSO. Long Term Storage: -20°C.
![Alkali Scientific D Luciferin Potassium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thiazolecarboxylic acid, potassium salt], 3 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660940.jpg-250.jpg)
Alkali Scientific D Luciferin Potassium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thiazolecarboxylic acid, potassium salt], 3 Grams
Luciferin potassium salt is a luminescent substrate used in bioluminescence assays. The substrate is oxidized by luciferase enzymes to produce light, making it ideal for cell assays, enzyme activity detection, and ATP measurements. This 3g quantity is highly concentrated and suitable for a variety of applications, including gene expression studies, cellular viability assays, and metabolic activity analysis. The bioluminescent reaction is highly sensitive and provides rapid results, allowing researchers to quantify enzyme activity and monitor cellular processes in real time. Luciferin is commonly used in biological research and diagnostic assays, offering a powerful tool for detecting low levels of biological activity.
![Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 500 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660986.jpg-250.jpg)
Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 500 Milligrams
D-Luciferin Free Acid is a high-purity biochemical reagent used in various laboratory applications, particularly in bioluminescence assays. It plays a crucial role in luciferase-based detection systems, where it acts as a substrate to produce light when catalyzed by luciferase enzymes. This reagent is used in a variety of applications such as biological research, molecular biology, and bioassays. The 500mg pack is perfect for laboratory use, ensuring precise and reliable results. It is essential for research in areas like cell signaling, gene expression studies, and protein quantification assays.
Biotium Coelenterazine CP
Coelenterazine cp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 15 times higher and has a faster response time to calcium compared to native coelenterazine.Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator. It binds coelenterazine to form an aequorin complex that emits light upon calcium binding and measures concentrations ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter of transcription regulation and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines are also used for chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues. Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines are also available.Properties : Yellow solid soluble in MeOH or EtOH; Store at -20°C, protect from light; C25H25N3O3; MW: 415.48.
Medchemexpress LLC Met-Gly-Pro-AMC | 1926163-52-1 | 99.7% | 460.55 | 5 MG
Met-Gly-Pro-AMC is a fluorescent peptide substrate of MetAP2. It is intended for research use only.
- Fluorescent peptide substrate of MetAP2
- Solid appearance, white to off-white
- Soluble in H2O at 50 mg/mL (108.57 mM) with ultrasonic
- Store powder at -80°C for 2 years, or -20°C for 1 year
- Store in solvent at -80°C for 6 months, or -20°C for 1 month
- Requires sealed storage, away from moisture
![Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 100 Mg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661849.jpg-250.jpg)
Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 100 Mg
D-Luciferin Free Acid is a bioluminescence substrate formulated for high-sensitivity detection in a range of biochemical assays. When oxidized by luciferase, D-Luciferin emits light, making it a valuable tool for detecting ATP levels, measuring cellular activity, or assessing enzyme activity. This 100mg product is designed for use in laboratories where precise and sensitive measurements are required. It is commonly used in applications involving molecular biology, microbiology, and cellular research, providing excellent luminescence signals for quantification and assay development in high-throughput systems.
![Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 50 Mg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660984.jpg-250.jpg)
Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 50 Mg
D-Luciferin Free Acid is a bioluminescence substrate used for high-sensitivity detection in biochemical assays. This reagent contains D-Luciferin, a substrate that is oxidized by the enzyme luciferase, resulting in light emission that can be measured for various applications. The free acid form is commonly used in bioluminescence assays for detecting ATP or monitoring cellular activity in research and clinical settings. This 50mg product is ideal for use in laboratories requiring high sensitivity for low-light detection, providing reliable and accurate results in a variety of biological assays and experimental setups.
MilliporeSigma TMB/E SINGLE REAGENT
TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate (soluble)
![Research Products International Corp Red-GAL [6-Chloro-3-indolyl-B-D-galactopyranoside], 100 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40040-0.1.JPG-250.jpg)
Research Products International Corp Red-GAL [6-Chloro-3-indolyl-B-D-galactopyranoside], 100 Milligrams
An alternative chromogenic substrate for β-D-galactosidase. Red-GAL is used in conjuction with IPTG for detection of β-galactosidase activity in bacterial colonies in a colorimetric assay to detect recombinants from non-recombinants.
Medchemexpress LLC Ac-DEVD-AMC | 169332-61-0 | MFCD00671411 | 99.2% | 675.64 | 5 MG
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treated with cell lysate, it releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
- Purity 99.18%
- Molecular weight 675.64
- Appearance as a solid
- Color white to off-white
- Emission wavelength 480 nm
- Excitation wavelength 390 nm
- Recommended powder storage: -80°C for 2 years, -20°C for 1 year
- Recommended storage in solvent: -80°C for 6 months, -20°C for 1 month
- In vitro solubility in DMSO: 10 mg/mL (14.80 mM)
Abcam Resorufin maltotriose, 5MG
MW 669.91 g/mol. Alpha-amylase substrate with red emission (λ max=585nm) after enzyme reaction. Useful for biofuels research.
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Z-Val-Lys-Met-AMC (TFA) | 141223-71-4 | 99.9% | 667.82 | 1 MG
Z-Val-Lys-Met-AMC (TFA) is a fluorescent substrate utilized for detecting β-secretase activity of cathepsin B.
- Fluorescent substrate for cathepsin B activity
- Appears as a white to off-white solid
- Store as powder at -80°C for up to 2 years
- Store in solvent at -80°C for 6 months, away from moisture
- Shipping at room temperature is suitable for durations less than 2 weeks
- For laboratory research use only
Medchemexpress LLC ATR kinase substrate peptide | 1232497-16-3 | 99.3% | 1814.05 | 1 MG
ATR kinase substrate peptide (ASELPASQPQPFSAKKK) is a peptide substrate for ATR protein kinase. It can be utilized to detect ATR kinase activity.
- Functions as a peptide substrate for ATR protein kinase
- Utilized to detect ATR kinase activity
- Has a Ki value of 6 nM against ATR
- Features the sequence shortening ASELPASQPQPFSAKKK
Medchemexpress LLC Gly-Arg-AMC | 65147-19-5 | 99.4% | 388.42 | 5 MG
Gly-Arg-AMC is a high-purity peptide substrate specifically designed for studying Dipeptidyl Aminopeptidase 1 (DPAP1) activity. It is provided in a solid, white to off-white appearance and is soluble in both DMSO and water. This substrate is essential for biochemical assays and research involving DPAP1, with specific storage recommendations to ensure long-term stability.
- Functions as a specific peptide substrate for DPAP1
- Supplied in a solid, white to off-white form
- High purity
- Soluble in DMSO and water
- Requires sealed storage away from moisture and light for optimal stability
- Stable as a powder at -80°C for 2 years or -20°C for 1 year
- Stable in solvent at -80°C for 6 months or -20°C for 1 month
AG Scientific Inc IPTG, 5 G
IPTG known as Isopropyl-Beta-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.CAS Number: 367-93-1Molecular Weight: 238.3 DaChemical Formula: C9H18O5SSolubility: Soluble in water (5%), renders clear colorless solutionStorage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.