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Substrates are laboratory reagents, chemicals, and consumables, and kits used to support or enhance biomedical, pharmaceutical, clinical, food science, environmental, and other research and testing laboratory processes. Products include enzymes, inhibitors, acids, bases, buffers, antibodies, and chromatography supplies.
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Boc-Leu-Gly-Arg-AMC is a fluorogenic AMC substrate primarily used for convertases. This compound is suitable for use in enzymatic assays. Research has shown its application in developing enzymatic assays to detect proteolytic enzymes, such as those capable of generating sodefrin, by measuring the liberation of 7-amino-4-methylcoumarin. The optimal activity for cleaving Boc-Leu-Gly-Arg-AMC in certain extracts has been observed at pH 9.0 and 45 °C.
Fluorogenic AMC substrate for convertases.
Can be used in enzymatic assays.
Suitable for demonstrating the presence of proteolytic enzymes.
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X-GAL (BCIG) is a widely used chromogenic -galactosidase substrate -galactosidase cleaves X-gal and produce an insoluble detectable blue compound X-Gal is applied in blue-white selection of recombinant bacterial colonies with the lac genotype
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An ALK5 and ALK4 inhibitor (IC50s 47 and 129 nM respectively) selective for ALK5 and ALK4 over a panel of 27 kinases at 10 UM inhibits ALK7-induced increases in phosphorylated SMAD2 levels in COS-1 cells overexpressing ALK7 at 5 UM inhibits TGF-B-induced but not TNF-a-induced apoptosis in Fao cells at 1 UM decreases IOP and subconjunctival immune cell infiltration in a rabbit model of trabeculectomy at 5 mg/eye
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A FAS-I inhibitor (IC50 79 nM) selective for FAS-I over 30 other enzymes receptors and ion channels at 10 UM inhibits the incorporation of 14C-acetate in mouse N-42 hypothalamus cells (IC50 570 nM) reduces the proliferation of GaMG human glioma cells at 10 UM
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