Substrates
Medchemexpress LLC D-luciferin sodium | 103404-75-7 | 99.9% | 302.30 | C11H7N2NaO3S2 | 1G
D-Luciferin sodium is the water-soluble sodium salt of D-luciferin, the natural substrate for firefly luciferase. Upon enzymatic oxidation it produces rapid yellow-green chemiluminescence (peak ~560 nm) used in reporter gene assays, ATP detection, and bioluminescence imaging. Supplied as a solid with high purity and multiple pack sizes, it should be stored protected from moisture and light.
- High purity (99.92%) for consistent assay results.
- Water-soluble sodium salt for easy preparation of solutions.
- Rapid yellow-green chemiluminescence with peak emission at ~560 nm.
- Suitable for reporter gene assays, ATP quantification, and imaging applications.
- Available in multiple pack sizes including 1 g.
- Stable when stored sealed at 4°C away from moisture and light.
Ubpbio BOC-LRR-AMC, 5MG
Fluorescent Peptides/Protein Substrates – BOC-LRR-AMC (UBPBio catalog# G3100, G3101) – Boc-LRR-AMC is a fluorogenic substrate for the trypsin – like activity of the 20S proteasome. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Medchemexpress LLC D-luciferin potassium | 115144-35-9 | 100.0% | 318.41 g·mol^-1 | C11H7KN2O3S2 | 1G
D-Luciferin potassium is the water-soluble potassium salt of D-luciferin, the natural substrate for firefly luciferase. It produces yellow-green bioluminescence upon ATP-dependent oxidation and is used for biochemical luciferase assays, cell-based reporter assays, and in vivo imaging. The compound is supplied as a solid powder suitable for preparation of aqueous solutions for experimental use.
- Water-soluble potassium salt for easy aqueous formulation.
- Suitable for bioluminescent luciferase assays and imaging.
- High analytical purity (manufacturer lists 99.97%).
- Molecular weight 318.41 g·mol⁻¹ and formula C11H7KN2O3S2.
- Available in multiple pack sizes including 1 g for laboratory use.
Cell Signaling Technology ChIP-Grade Protein G Agarose Beads 1 ml
ChIP-Grade Protein G Agarose Beads 1 ml
Medchemexpress LLC D-luciferin sodium | 103404-75-7 | MFCD00044938 | >95.0% | 302.3 g/mol | C11H7N2NaO3S2 | 100MG
D-luciferin sodium is the water-soluble sodium salt of firefly luciferin used as a chemiluminescent substrate for luciferase assays in vitro and in vivo. Supplied as a powder, it dissolves readily in aqueous or organic solvents to prepare assay solutions for reporter gene assays, bioluminescence imaging, and ATP-dependent measurements.
- Water-soluble sodium salt suitable for luciferase-based bioluminescence assays.
- High purity (>95%) for reliable assay performance.
- Soluble in water and DMSO (solubility reported to ~25 mM) for easy preparation.
- Provided as a lyophilized powder for accurate dosing and storage.
- Stable when stored desiccated at -20°C and protected from light.
Medchemexpress LLC Z-Leu-Leu-Glu-AMC | 348086-66-8 | MFCD01074989 | 99.5% | 664.75 | C35H44N4O9 | 25MG
Z-Leu-Leu-Glu-AMC is a fluorogenic tripeptide substrate used to measure postacidic-like (peptidylglutamyl) hydrolyzing activity of the 20S proteasome. Proteolytic cleavage releases the fluorescent 7-amino-4-methylcoumarin (AMC) reporter, enabling quantitative, sensitive measurement of proteasome activity in biochemical and enzymatic assays.
- Fluorogenic substrate for proteasome activity assays.
- Releases AMC reporter for sensitive fluorescence detection.
- Suitable for biochemical and enzymatic assay development.
- High purity for reproducible assay performance.
- Available in small research-scale quantities.
ACROBiosystems Human FcRn binding Kit (TR-FRET) is expressed from Human, 100 tests
Human FcRn binding Kit (TR-FRET) is expressed from Human
Medchemexpress LLC Boc-Gln-Arg-Arg-AMC acetate | 2223665-74-3 | 99.25% | 775.85 | 10 MG
Boc-Gln-Arg-Arg-AMC acetate is a fluorogenic substrate utilized for determining protease activity. Upon hydrolysis, it releases the fluorescent product 7-amino-4-methylcoumarin (AMC), which emits a fluorescent signal under UV light. This product is for research use only.
- Fluorogenic substrate
- Used for determining protease activity
- Releases fluorescent product 7-amino-4-methylcoumarin (AMC) upon hydrolysis
- Emits fluorescent signal under UV light
Research Products International Corp D-Luciferin Potassium Salt 3 G
RPI offers the highest purity D-Luciferin Potassium Salt for a wide range of bioluminescent applications. Produces bioluminescence when oxidized by firefly luciferase. Allows highly sensitive bioluminescent measurements. Common research uses reporter gene assays, ATP assays, whole animal studies, DNA sequencing and others. Our product is specially purified and tested extensively to ensure the highest signal and reproducibility with low background and sensitivity. Guaranteed purity is determined by both standard HPLC and Chiral HPLC methods. Protect from light and moisture.
Research Products International Corp D-Luciferin Sodium Salt 10 G
RPI offers the highest purity D-Luciferin Sodium Salt for a wide range of bioluminescent applications. Allows highly sensitive bioluminescent measurements. Ideal for reporter gene assays, ATP assays, whole animal studied, DNA sequencing and more. Our product is specially purified and tested extensively to ensure the highest signal and reproducibility with low background and sensitivity. Guaranteed purity is determined by both standard HPLC and Chiral HPLC methods. Protect from light and moisture.
Prolume Ltd h-Coelenterazine-SOL (in vivo), 1 mg
Water-soluble h-Coelenterazine is formulated to be water-soluble and safe for in vivo imaging applications with Renilla Luciferase.
Iwai North America IMATRIX-511 1050UG 175UG X6
iMatrix-511 is a recombinant Laminin- 511 E8 fragment expressed by CHO-cell. Laminin-511 E8 fragments allow ES/iPS cells and MSCs to be maintained in xeno-free culture conditions. It also enables the passaging of single cells and provides greater adhesion than full-length Laminin, Vitronectin, or Matrigel.