Substrates
Medchemexpress LLC Boc-Gln-Arg-Arg-AMC acetate | 2223665-74-3 | 99.25% | 775.85 | 10 MG
Boc-Gln-Arg-Arg-AMC acetate is a fluorogenic substrate utilized for determining protease activity. Upon hydrolysis, it releases the fluorescent product 7-amino-4-methylcoumarin (AMC), which emits a fluorescent signal under UV light. This product is for research use only.
- Fluorogenic substrate
- Used for determining protease activity
- Releases fluorescent product 7-amino-4-methylcoumarin (AMC) upon hydrolysis
- Emits fluorescent signal under UV light
Ubpbio SUC-LLVY-AMC, 25MG
Fluorescent Peptides/Protein Substrates – SUC-LLVY-AMC (UBPBio catalog# G1100, G1101) – Suc-LLVY-AMC is a fluorogenic substrate for the chymotrypsin – like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Ubpbio BOC-LRR-AMC, 5MG
Fluorescent Peptides/Protein Substrates – BOC-LRR-AMC (UBPBio catalog# G3100, G3101) – Boc-LRR-AMC is a fluorogenic substrate for the trypsin – like activity of the 20S proteasome. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Neogen Diagnostics Enhanced K-Blue TMB Substrate, 200 mL, Microwell, HRP, Colorimetric
Enhanced K-Blue is a one bottle stabilized chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3,5,5 tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). Enhanced K-Blue develops a deep blue color in the presence of peroxidase labelled conjugate. Enhanced K-Blue has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate. Stable for a minimum of 48 months when stored at 2-8C.
Cayman Chemical ADHP Fluorescent
A highly sensitive, stable substrate for HRP that reacts with H2O2 to produce the fluorescent compound resorufin; enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl samples
Medchemexpress LLC HER2/CD340 Human B 20ug
The HER2/CD340 protein is a dynamic tyrosine kinase that is essential in the neuregulin receptor complex and regulates microtubule dynamics Upon activation it triggers the MEMO1-RHOA-DIAPH1 pathway inhibits GSK3B and promotes the association of APC and CLASP2 on the cell membrane to achieve microtubule stabilization HER2/CD340 Protein Human (Biotinylated HEK293 Fc-Avi) is the recombinant human-derived HER2/CD340 protein expressed by HEK293 with C-Avi C-hFc labeled tag
Sino Biological Peptide Substrates: Abltide
The Abltide peptide sequence (EAIYAAPFAKKK) is based on the C-terminus of Abl.
Bioworld X-Gal-IPTG, Ready-to-Use Spray, 20 mL
X-Gal-IPTG, Ready- to-use, SprayStable X-gal/IPTG non-toxic solution For use in blue/white colony screening in expression studiesDoes not contain toxic DMF and DMSOContains 40 mg/ml of IPTG (Isopropyl-β-D-Thiogalactopyranoside) and 40 mg/ml of X-gal (5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside)Supplied in a very easy to use and convenient to use Spray BottleAlso available in a Dropper Bottle (cat# 21530077-1)Use: Spray 2-3 times on desired bacterial plate Spread and ensure absorption (wait ~5-10 minutes) Plate the transformed bacteria to be tested and incubate
Medchemexpress LLC Suc-AAPF-AMC | 88467-45-2 | MFCD00080246 | 99.5% | 661.70 | C34H39N5O9 | 5 MG
Suc-AAPF-AMC is a fluorogenic peptide substrate used to quantify α-chymotrypsin activity. The succinylated tetrapeptide releases 7-amino-4-methylcoumarin (AMC) upon proteolytic cleavage, producing a fluorescent signal suitable for kinetic or end-point assays. The product is supplied with purity data, a product data sheet, and a safety data sheet for handling and storage.
- Fluorogenic substrate for α-chymotrypsin activity assays
- AMC release produces a measurable fluorescent signal for kinetic and end-point assays
- Available as a solid and as a 10 mM solution in DMSO for assay flexibility
- High purity suitable for research applications
- Includes datasheet and safety data sheet for use and storage guidance
Medchemexpress LLC Boc-LRR-AMC | 5MG
Boc-LRR-AMC (Boc-Leu-Arg-Arg-AMC) is a fluorogenic substrate which can be utilized for detecting the trypsin-like activity of the 26S proteasome or the 20S proteasome core[1]
Medchemexpress LLC D-luciferin potassium | 115144-35-9 | 100.0% | 318.41 g·mol^-1 | C11H7KN2O3S2 | 1G
D-Luciferin potassium is the water-soluble potassium salt of D-luciferin, the natural substrate for firefly luciferase. It produces yellow-green bioluminescence upon ATP-dependent oxidation and is used for biochemical luciferase assays, cell-based reporter assays, and in vivo imaging. The compound is supplied as a solid powder suitable for preparation of aqueous solutions for experimental use.
- Water-soluble potassium salt for easy aqueous formulation.
- Suitable for bioluminescent luciferase assays and imaging.
- High analytical purity (manufacturer lists 99.97%).
- Molecular weight 318.41 g·mol⁻¹ and formula C11H7KN2O3S2.
- Available in multiple pack sizes including 1 g for laboratory use.
Medchemexpress LLC Ac-PAL-AMC | 1431362-79-6 | 1MG
Ac-PAL-AMC is a fluorogenic substrate specific for 20S proteasome LMP2/ 1i activity[1]
Medchemexpress LLC Z-leu-leu-glu-AMC | 348086-66-8 | MFCD01074989 | 99.5% | 664.75 g/mol | C35H44N4O9 | 1MG
Z-Leu-Leu-Glu-AMC is a fluorogenic tripeptide substrate used to measure post-acidic (caspase-like) hydrolytic activity of the 20S proteasome. Proteolytic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal suitable for kinetic assays, inhibitor screening, and proteasome activity profiling in biochemical research.
- High purity (≈99.5%) for reliable assay performance.
- Releases AMC for sensitive fluorometric detection.
- Suitable for 20S/26S proteasome activity and inhibitor assays.
- Available as solid and 10 mM DMSO solution formats.
- Supplied in small research pack sizes for convenient use.