Substrates
Gold Biotechnology Inc X-gluc (CHX salt) 2 x 1 g
X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate. X-Gluc is often used in GUS staining of plant cells and tissue and in infections caused by E. coli.
Gold Biotechnology Inc D-Luciferin, Potassium Salt (Proven and Published™) 2 x 1 g
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Cell Signaling Technology ChIP-Grade Protein G Agarose Beads 1 ml
ChIP-Grade Protein G Agarose Beads 1 ml
Cell Signaling Technology ChIP-Grade Protein G Magnetic Beads 1 ml
ChIP-Grade Protein G Magnetic Beads 1 ml
Vector Laboratories VECTOR Blue Alkaline Phosphatase (AP) Substrate Kit 200 ml
VECTOR Blue Alkaline Phosphatase (AP) Substrate Kit produces a blue, heat-stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Sections stained with the VECTOR Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount) must be used because the reaction product is soluble in xylene. VECTOR Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. The stock solutions in this kit are provided in convenient dropper bottles, with sufficient volume for 200 ml working solution.
Iwai North America IMATRIX-511 1050UG 175UG X6
iMatrix-511 is a recombinant Laminin- 511 E8 fragment expressed by CHO-cell. Laminin-511 E8 fragments allow ES/iPS cells and MSCs to be maintained in xeno-free culture conditions. It also enables the passaging of single cells and provides greater adhesion than full-length Laminin, Vitronectin, or Matrigel.
Medchemexpress LLC Solutol HS-15, 61909-81-7, 50 g
Formula: (C₂H₄O)nC₁₈H₃₆O3. Solutol HS-15 is a permeability enhancer. Store at 4°C, sealed storage, away from moisture.
Medchemexpress LLC Ac-ANW-AMC | 2357123-49-8 | 99.4% | 10 MG
Ac-ANW-AMC is a fluorogenic peptide substrate used to measure immunoproteasome β5i activity in vitro. Proteolytic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal suitable for kinetic or end-point assays.
- releases AMC fluorophore upon proteolytic cleavage for fluorescence detection.
- reported excitation/emission: 380 nm/460 nm; assay notes recommend 345 nm/445 nm for β5i measurements.
- high purity (99.4%) for reliable biochemical assays.
- molecular weight 588.61 g/mol; formula C30H32N6O7.
- available in small quantities (for example, 10 mg) for research use.
- store at -20°C and protect from light; in solution, store at -80°C for long-term stability.
Enzo Life Sciences Suc-Leu-Leu-Val-Tyr-AMC (5 mg) CAS: 94367-21-2
Proteasome substrate. Fluorogenic substrate for 20S proteasome (Prod. No. BML-PW8720) and other chymotrypsin-like proteases, as well as calpains. Ex.: 380nm Em.: 460nm. Alternative Name: Suc-LLVY-AMC, Proteasome substrate (fluorogenic). Calpain substrate (fluorogenic). Formula: C40 H53 N5 O10. MW: 763.9. CAS: 94367-21-2. Formulation: Lyophilized. Purity: ≥97% (HPLC). Sequence N-Succinyl-Leu-Leu-Val-Tyr-AMC (AMC=7-amino-4-methylcoumarin). Solubility: Soluble in DMSO (at least 5mM). Long Term Storage: -20°C. Handling: Protect from light.
Carbosynth LLC. D-Luciferin Firefly, sodium salt monohydrate, 103404-75-7, MFCD00044938, 1 g
Formula: C11H9N2NaO4S2, Molecular weight: 320.32 g/mol, 98%, Store at -15°C, Synonyms: (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt.
Sigma Aldrich Fine Chemicals Biosciences 4-Methylumbelliferyl phosphate | 3368-04-5 | MFCD00016969 | 250mg
4-Methylumbelliferyl phosphate | MW: 256.15 | 3368-04-5 | MFCD00016969 | 250mg
Medchemexpress LLC D-luciferin (potassium) | 115144-35-9 | 99.1% | 318.41 g/mol | C11H7KN2O3S2 | 500MG
D-Luciferin potassium is the potassium salt of D-luciferin, the native substrate for firefly luciferase. It is provided as a water-soluble reagent for use in in vitro and in vivo bioluminescence assays and imaging, intended to deliver consistent luminescent signals with appropriate storage for stability.
- Water-soluble potassium salt form suitable for aqueous assays.
- Natural substrate for luciferase-based bioluminescent detection.
- High purity (99.1% by LCMS) for reproducible assay results.
- Molecular weight 318.41 g/mol enables precise dosing.
- Stable when stored sealed at 4°C; in solvent stable at -80°C for up to 2 years.
- Suitable for reporter assays and in vivo bioluminescence imaging.
Medchemexpress LLC Boc-Gln-Arg-Arg-AMC acetate | 2223665-74-3 | 99.25% | 775.85 | 10 MG
Boc-Gln-Arg-Arg-AMC acetate is a fluorogenic substrate utilized for determining protease activity. Upon hydrolysis, it releases the fluorescent product 7-amino-4-methylcoumarin (AMC), which emits a fluorescent signal under UV light. This product is for research use only.
- Fluorogenic substrate
- Used for determining protease activity
- Releases fluorescent product 7-amino-4-methylcoumarin (AMC) upon hydrolysis
- Emits fluorescent signal under UV light