Substrates
MOLECULAR DEPOT LLC Substrate Buffer - 50 mL
Beta-Galactosidase Chromogenic Substrate Buffer is a highly optimized buffer for the specific stabilization of beta-galactosidase chromogenic substrates This product has been used as stabilizing agent in various beta-galactosidase-related applications For in vitro research use only
![Research Products International Corp NBT [Nitro Blue Tetrazolium] [2,2'-Di-P-Nitrophenyl-5, 5'-Diphenyl-3, 3'-(3,3'-Dimethoxy-4, 4'-Diphenylene)-Ditetrazolium Chloride], 100 MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-N66000-0.1.JPG-250.jpg)
Research Products International Corp NBT [Nitro Blue Tetrazolium] [2,2'-Di-P-Nitrophenyl-5, 5'-Diphenyl-3, 3'-(3,3'-Dimethoxy-4, 4'-Diphenylene)-Ditetrazolium Chloride], 100 MG
Colorimetric substrate for dehydrogenases and other oxidases. NBT is used with X-Phos for colorimetric detection of alkaline phosphatase activity in blotting, immunohistochemical and cytochemistry techniques. Protect from light.
Research Products International Corp 4-Nitrophenyl-B-D-Glucopyranoside, 5 Grams
Spectrophotometric substrate for detection of β-D-Glucosidase.
Free PNP: <200ppm
Biotium NucView 488 Caspase-3 Substrate, 1mM in 1X PBS
NucView Caspase-3 Substrates are novel fluorogenic substrates for real-time or endpoint detection of apoptosis in live cells. The non-toxic, no-wash substrates do not inhibit caspase-3 unlike other caspase substrates or FLICA assays permitting multiday analysis. Consisting of a high-affinity DNA dye linked to a DEVD caspase-3 substrate moiety, the cell permeable non-fluorescent substrate is cleaved by caspase-3 in apoptotic cells to emit fluorescence, allowing detection of both caspase-3 activity and apopototic nuclear morphology. Suitable for flow cytometry, fluorescence microscopy, or live cell imaging systems, staining is compatible with fixation and permeabilization for immunostaining. NucView 488 is validated in over 100 cell types and 200 publications. Available with blue (NucView 405), green (NucView 488), or red (NucView 530) fluorescence, in DMSO or PBS for DMSO-sensitive cells, and as kits with apoptosis or necrosis probes. NucView technology is covered by U.S. patents.
Biotium NucView 530 Caspase-3 Substrate, 1 mM in DMSO, Trial Size
NucView Caspase-3 Substrates are novel fluorogenic substrates for real-time or endpoint detection of apoptosis in live cells. The non-toxic, no-wash substrates do not inhibit caspase-3 unlike other caspase substrates or FLICA assays permitting multiday analysis. Consisting of a high-affinity DNA dye linked to a DEVD caspase-3 substrate moiety, the cell permeable non-fluorescent substrate is cleaved by caspase-3 in apoptotic cells to emit fluorescence, allowing detection of both caspase-3 activity and apopototic nuclear morphology. Suitable for flow cytometry, fluorescence microscopy, or live cell imaging systems, staining is compatible with fixation and permeabilization for immunostaining. NucView 488 is validated in over 100 cell types and 200 publications. Available with blue (NucView 405), green (NucView 488), or red (NucView 530) fluorescence, in DMSO or PBS for DMSO-sensitive cells, and as kits with apoptosis or necrosis probes. NucView technology is covered by U.S. patents.
Gold Biotechnology Inc D Luciferin Sodium Salt 250mg
Tested and Certified for in vivo imaging (See Luciferin FAQ in additional information)
Gold Biotechnology Inc D Luciferin Potassium Salt 4g
L uciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2 as cofactors to emit a characteristic yellow-green emission in the presence of oxygen which shifts to red light in vivo at 37 C Through the utilization of ATP the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain
Sino Biological Peptide Substrates: Histone H1 Peptide
The Histone H1 Peptide (152-159) sequence (GGGPATP-KKAKKL-COOH) is derived from human histone H1 between amino acids 152-159 and is suitable for use as the substrate for CDK family kinase assays, such as CDK1, CDK2, CDK5 and CDK6.
Aniara Diagnostica LLC BIOPHEN CS-01(38) THROMB 12 EA
This item MFG by HYPHEN BioMed and Dis. by Aniara Diagnostics. Chromogenic substrate for thrombin, lyophilized. Peptide sequence H-D-Phe-Pip-Arg-pNa, 2HCl. 12 vials x 25 mg.
Medchemexpress LLC D-luciferin sodium salt | 103404-75-7 | 99.9% | 302.30 g/mol | C11H7N2NaO3S2 | 200MG
D-Luciferin sodium is the sodium salt of D-luciferin, the natural substrate for firefly luciferase. Upon oxidation by luciferase it produces bioluminescence, making it suitable for in vitro and in vivo imaging and quantitative luciferase assays. The material is supplied as a solid for preparation of aqueous stock solutions and experimental use.
- High purity (~99.9%).
- Water-soluble sodium salt form suitable for biological assays.
- Enables bioluminescent imaging and quantitative luciferase assays.
- Molecular weight 302.30 g/mol.
- Available in multiple pack sizes from 5 mg to 50 g.
Ubpbio K48-linked di-Ubiquitin (human), enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
Ubpbio K63-linked polyubiquitin chain mix containing 2-12 human ubiquitin, enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
Enzo Life Sciences Digoxigenin-UTP, alkali-stable (250nmol)
Digoxigenin-UTP can replace UTP in reactions catalyzed by T3, T7 or SP6 RNA polymerases. The digoxigenin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids. The single-stranded RNA probes offer selectivity unavailable with double-stranded DNA probes, because they are strand-specific. Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. Alternative name: Digoxigenin-11-UTP. Concentration: 10mM. Quantity: 25µl. Purity: ≥93% (HPLC). Formulation: Liquid. Solution in water.
Medchemexpress LLC Meosuc-aapv-amc | 72252-90-5 | MFCD00077135 | 98.1% | 627.69 g/mol | C31H41N5O9 | 5 MG
MeOSuc-AAPV-AMC is a fluorogenic peptide substrate that releases 7-amino-4-methylcoumarin (AMC) upon proteolytic cleavage, producing a measurable fluorescence signal for elastase activity assays (Ex 380 nm, Em 460 nm). Intended for research use in enzymatic and kinetic studies.
- Fluorogenic substrate for human leukocyte and porcine pancreatic elastase.
- Generates AMC fluorescence at Ex 380 nm and Em 460 nm.
- Reported Km for elastase: 362 μM.
- High purity (98.07%) suitable for analytical assays.
- Soluble in DMSO at 100 mg/mL; ultrasonication recommended.
- White to off-white solid; powder storage at low temperature preserves stability.
- Provided in milligram quantities for assay development and validation.