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Regeneration or ActivationA saturated molecular sieve can be restored to its original capacity by regeneration the principle of which involves changing the conditions surrounding the adsorbent to correspond to a very low equilibrium capacity. In general the greater the difference between the equilibrium capacities of adsorption and regeneration the more rapid and complete the regeneration.The sieve may be regenerated in one of four ways1. Thermal reactivation -The maximum regeneration temperature for Silica is 300C.2. Pressure reactivation3. Passing an appropriate fluid through the gel bed at normal temperature and pressure.4. Displacement of adsorbates by passing a high concentration of molecules in a fluid through the bed.
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Nutlin-3a ((-)-Nutlin-3) the active enantiomer of Nutlin-3 inhibits the p53/MDM2 interaction with IC50 of 90 nM in a cell-free assay Nutlin-3a induces autophagy and apoptosis in a p53-dependent manner
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Molecular sieves are crystalline metal aluminosilicates having a 3-dimensional interconnecting network of silica and alumina tetrahedra. Natural water of hydration is removed from this network by heating to produce uniform cavities which selectively adsorb molecules of a specific size. The 3A form is made by substituting potassium cations for the inherent sodium ions of the 4A structure reducing the effective pore size to ~3A… excluding diameter 3A… e.g. ethane. The 4 to 8-mesh type is normally used in gas-phase applications. Molecular sieves are considered a universal drying agent in polar and nonpolar media.
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SMI-4a (CAS 438190-29-5) is a selective inhibitor of the serine/threonine kinase Pim-1 exhibiting an IC50 of 17 nM Pim-1 is implicated in the regulation of cell cycle progression and apoptosis with its overexpression associated with enhanced cell survival in various malignancies SMI-4a demonstrates increased activity compared to related compounds and inhibits downstream mTORC1 signaling and activates AMPK in leukemia cell lines leading to cell cycle arrest and apoptosis induction In preclinical studies SMI-4a reduced tumor volume in xenograft mouse models highlighting its utility for investigating Pim kinase-driven pathways in cancer research
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Recombinant human Semaphorin-3A is a secreted guidance protein produced in HEK293 mammalian cells with an N-terminal His tag and supplied as a small research pack (2 μg). The product preserves native glycosylation and folding, exhibits an observed molecular weight of 60-73 kDa by SDS-PAGE, and has low endotoxin levels suitable for many cell-based and biochemical assays. Aliquot and store frozen to maintain activity.
Preserves mammalian glycosylation and native folding
Low endotoxin suitable for cell-based assays
N-terminal His tag simplifies purification and detection
Small research pack size ideal for pilot studies
Observed molecular weight 60-73 kDa due to glycosylation
Stable when aliquoted and stored frozen
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