Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human IL-23 / IL23R Binding Kit Standard
HTRF Human IL-23 / IL23R Binding Kit Standard
Revvity Health Sciences Inc HTRF Human Total BRD2 Detection Kit, 10,000 Assay Points
HTRF Human Total BRD2 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total ULK1 Detection Kit, 10,000 Assay Points
HTRF Human Total ULK1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF EPIgeneous Binding Domain B Detection Kit, 10,000 Assay Points
HTRF EPIgeneous Binding Domain B Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total RBM39 Detection Kit, Control Lysate
HTRF Human & Mouse Total RBM39 Detection Kit, Control Lysate
Molecular Devices Laptop, Dell Latitude D620, 1.83 GHz, 2 GB RAM
The Molecular Devices PC Laptop Bundle (0310-4874) provides a ready-to-use laptop configured for instrument control, data acquisition, and basic office tasks when used with microplate readers.
- Dual-core 1.83 GHz processor.
- 2 GB RAM.
- 80 GB hard drive.
- 24x CD-RW/DVD optical drive.
- 14.1 in WXGA display.
- Includes office productivity and PDF software.
- Configured for microplate reader instrument control and data acquisition.
Sigma Aldrich Fine Chemicals Biosciences Duolink(R) In Situ Wash Buffers, Fluorescence | 20L
Duolink(R) In Situ Wash Buffers, Fluorescence | 20L
Revvity Health Sciences Inc HTRF Human Total JAK1 Detection Kit, 10,000 Assay Points
HTRF Human Total JAK1 Detection Kit, 10,000 Assay Points
Molecular Devices Polarization Filter, 700 nm BW 60 nm, Vertical
This fluorescence polarization filter in a holder enables near-infrared fluorescence polarization measurements centered at 700 nm and installs into the filter slot of compatible multi-mode microplate readers to support FP assays.
- 700 nm center wavelength with 60 nm bandwidth.
- Fluorescence polarization optical design for polarization-based measurements.
- Vertical polarization orientation for polarization detection.
- Mounted in a holder for insertion into reader filter slots.
- Intended for fluorescence polarization assays and near-IR detection workflows.
Revvity Health Sciences Inc HTRF Human & Mouse Ataxin 2 Detection Kit Standard
HTRF Human & Mouse Ataxin 2 Detection Kit Standard
Revvity Health Sciences Inc HTRF Human IL-2 High Performance Detection Kit Standard
HTRF Human IL-2 High Performance Detection Kit Standard
Revvity Health Sciences Inc HTRF Human and Mouse Total IKZF1 Detection Kit, 500 Assay Points
HTRF Human and Mouse Total IKZF1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human IL-23 / IL12R-β1 Binding Kit, 10,000 Assay Points
HTRF Human IL-23 / IL12R-β1 Binding Kit, 10,000 Assay Points