Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF AAV8 Detection Kit, 10,000 Assay Points
HTRF AAV8 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA Surefire Ultra Human Phospho-NPM (Thr199) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Human Phospho-NPM (Thr199) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human XIAP / BIR3 Binding Kit Standard
HTRF Human XIAP / BIR3 Binding Kit Standard
Revvity Health Sciences Inc HTRF Human Androgen Receptor Full Length Detection Kit, 10,000 Assay Points
HTRF Human Androgen Receptor Full Length Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit, 100,000 Assay Points
HTRF Human IL-1β High Performance Detection Kit, 100,000 Assay Points
Molecular Devices Emission Filter, 465 nm, 35 nm Bandwidth
This emission filter is centered at 465 nm with a 35 nm bandwidth and is designed for fluorescence detection in multi-mode microplate readers. It enables sensitive measurement of blue-emitting fluorophores and serves as a direct replacement filter for compatible reader filter modules.
- Passes emission centered at 465 nm, 35 nm bandwidth.
- Suitable for detection of blue-fluorescent dyes and probes.
- Intended for use in fluorescence-based microplate assays.
- Replacement format designed for plug-in filter modules.
- Helps improve assay specificity by isolating emission band.
Molecular Devices Fluorescence Polarization Filter, 635 nm, 35 nm BW
Molecular Devices fluorescence polarization filter specified for a 635 nm center wavelength with a 35 nm bandwidth. It is designed for fluorescence polarization assays in microplate and ELISA readers and is supplied with a holder for insertion into compatible filter modules.
- Suitable for fluorescence polarization measurements.
- 635 nm center wavelength, 35 nm bandwidth.
- Polarized horizontal orientation.
- Includes holder for filter module insertion.
- Intended for microplate and ELISA reader applications.
Molecular Devices Fluorescence Polarization Filter, 625 nm, 35 nm, Vertical
Fluorescence polarization filter element with a 625 nm center wavelength and 35 nm bandwidth, polarized vertical, designed as an accessory for compatible microplate readers to enable fluorescence polarization assays.
- 625 nm center wavelength.
- 35 nm bandwidth.
- Vertical polarization orientation.
- Designed for fluorescence polarization assays.
- Compatible with microplate reader accessory filter holders.
- Easy installation into the reader filter slot.
Revvity Health Sciences Inc HTRF Human & Mouse Total RBM39 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total RBM39 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK3 Detection Kit, Control Lysate
HTRF Human Total JAK3 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 50,000 Assay Points
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF High Performance Human IL-6 Detection Kit, Standard, 3 vials
HTRF High Performance Human IL-6 Detection Kit, Standard, 3 vials
Revvity Health Sciences Inc HTRF Human Total ATG14 Detection Kit, 500 Assay Points
HTRF Human Total ATG14 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit Standard, 3 Vials
HTRF Human IL-1β High Performance Detection Kit Standard, 3 Vials