Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
Monochromator- or filter-based wavelength selection
Visible only or UV/Vis spectrum, fluorescence, and luminescence
Multiple photomultiplier tubes (PMTs) for simultaneous assays
Temperature and CO2/O2controls
Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Offers budget-friendly, advanced detection capabilities. BioTek Epoch Microplate Spectrophotometer is an optical reader which provides precise UV/Vis measurements in one compact, highly versatile unit.
Take3 and Take3 Trio Micro-Volume plates expand functionality of the BioTek microplate spectrophotometers, multi-mode and Hybrid readers, while reducing sample volumes, costs and time required for routine quantification
Flexible monochromator-based detection with high performance dichroic-based filter detection. BioTek Synergy H1 Hybrid Multi-Mode Monochromator Fluorescence Microplate Readers with its monochromator optics supports top and bottom fluorescence intensity, UV-visible absorbance and high-performance luminescence detection.
Flexible monochromator-based detection with high performance dichroic-based filter detection. BioTek Synergy H1 Hybrid Multi-Mode All Detection Modes Microplate Readers have monochromator optics which supports top and bottom fluorescence intensity, UV-visible absorbance and high-performance luminescence detection.