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A lignan with diverse biological activities; inhibits the binding of PAF to isolated rabbit platelet plasma membranes (IC50 = 4.5 µM); inhibits RANKL- and M-CSF-induced osteoclast formation in mouse bone marrow-derived macrophages at 3-100 µM; protects against cytotoxicity induced by human Aβ (25-35) in primary rat hippocampal neurons at 10 µM; reduces acetic acid-induced abdominal constrictions in mice and carrageenan-induced paw edema in rats at 20 mg/kg
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Alkaline phosphatase catalyzes the removal of phosphate group from various compounds that are phosphorylated. Alkaline phosphatase from calf intestine hydrolyzes 5AE-monophosphate groups from both DNA and RNA. It can also hydrolyze 5AE-diphosphate and 5AE-triphosphate groups from RNA.
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rAPid Alkaline Phosphatase is an alkaline phosphatase isolated from bovine intestine and supplied as a recombinant enzyme of 56 kD expressed in the yeast Pichia Pastoris. The recombinant form ensures consistency and safety. rAPid Alkaline Phosphatase catalyzes the dephosphorylation of 5 phosphates from DNA and RNA nucleotides and proteins. Alkaline phosphatase treatment prevents self-ligation of DNA fragments by removing the 5-phosphoryl termini required by ligases this feature is of major importance in cloning strategies to decrease vector background. Unlike calf intestinal phosphatase rAPid Alkaline Phosphatase is rapidly completely and irreversibly inactivated by heat treatment for two minutes at 75 C. It is therefore an excellent alternative to Shrimp Alkaline Phosphatase. In addition the enzyme is active in restriction enzyme buffers. Therefore restriction enzyme digestion dephosphorylation enzyme inactivation ligation or 5-end labeling can be performed without purif
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