Flow Cytometry Analyzer Supplies
Apexbio Technology LLC LUMI ATP CELL VIAB ASSAY 1000T
Luminescent ATP Cell Viability Assay Kit I uses thermostable firefly luciferase and purified luciferin substrate to quantitatively measure cellular ATP levels an indicator closely correlated with viable cell numbers and metabolic activity Upon reagent addition cells rapidly lyse to release intracellular ATP which interacts with luciferase and luciferin substrate generating a luminescent signal directly proportional to the number of viable cells The protocol does not require prior cell lysis steps offering a streamlined assay completed within 10 minutes This assay is suitable for detecting viability ranging from 10 to 30 000 cells and provides comparable results to common commercial ATP-based viability assays such as CellTiter-Glo
Bellco Glass, Inc. Drive stem assembly with 3/4¿OD shaft for use with 7L marine and pitched blade impellers. Fits into 27 mm center port of 7L metric headplates. Multiple impellers may be assembled onto shaft as required.
Drive stem assembly with 3/4¿OD shaft for use with 7L marine and pitched blade impellers. Fits into 27 mm center port of 7L metric headplates. Multiple impellers may be assembled onto shaft as required.
Cytek Biosciences Inc Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X)
Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) ..... Cat. No. TNB-4970-UL400 ..... Size 400 µL
Enzo Life Sciences CYTO-ID Green long-term cell tracer kit (1Kit)
CYTO-ID Green long-term cell tracer kit uses proprietary noncovalent cell labeling technology to stably incorporate a green fluorescent dye containing hydrophobic aliphatic chains into the cell membrane’s lipid bilayer. The dye may be loaded into cells by following the included protocol. The labeling buffer is isotonic for mammalian cells and contains no detergents or organic solvents. The appearance of labeled cells may vary depending upon the cell type from uniformly bright to punctuate. This difference is thought to relate to the extent of membrane internalization occurring after cell labeling. The CYTO-ID Green tracer dye fluorescence is independent of pH within normally encountered physiologic ranges and fluorescence intensity per cell is typically unaffected by the ultimate pattern of dye distribution. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection .
Biolegend Purified anti-FOXP3 [W23223B] Isotype Rat IgG2a Reactivity Human Apps IHC-P WB ICFC Size 25 g
Purified anti-FOXP3 [W23223B] Isotype Rat IgG2a Reactivity Human Apps IHC-P WB ICFC Size 25 g
AdipoGen JC-10 (high purity), 1 mg
MW: ~600, ≥98%, AppearanceDeep blue to violet solid. JC-10 is superior analog of JC-1, useful for determining mitochondrial membrane potential (MMP) in cells by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 is a cationic, lipophilic dye that accumulates in mitochondria of cells with a polarized mitochondrial membrane, selectively generating an orange J-aggregate emission profile (λex = 540 nm/λem = 590 nm) in healthy cells.
Biotium Live-or-Dye™ Staining Kit 488/515 50 Assays
Live-or-Dye™ Fixable Viability Staining Kits are cell membrane impermeable amine-reactive dyes designed for discrimination between live and dead cells during flow cytometry. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. Live-or-Dye™ 488/515 is suitable for excitation by 488 nm laser line. This trial size kit has sufficient reagent for 50 assays.
Biotium Live-or-Dye™ 750/777 Fixable Viability Staining Kit, Trial Size (50 assays)
Live-or-Dye™ Fixable Viability Staining Kits are cell membrane impermeable amine-reactive dyes designed for discrimination between live and dead cells during flow cytometry. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. Live-or-Dye™ 750/777 is suitable for excitation by 633 nm or 640 nm laser line. This trial size kit has sufficient reagent for 50 assays.
Biotium Live-or-Dye™ 568/583 Fixable Viability Staining Kit (200 assays)
Live-or-Dye™ Fixable Viability Staining Kits are cell membrane impermeable amine-reactive dyes designed for discrimination between live and dead cells during flow cytometry. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. Live-or-Dye™ 568/583 is suitable for excitation by 488 nm or 561 nm laser line. This kit has sufficient reagent for 200 assays.
Biotium Live-or-Dye™ 405/452 Fixable Viability Staining Kit (200 assays)
Live-or-Dye™ Fixable Viability Staining Kits are cell membrane impermeable amine-reactive dyes designed for discrimination between live and dead cells during flow cytometry. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. Live-or-Dye™ 405/452 is suitable for excitation by 405 nm laser line. This kit has sufficient reagent for 200 assays.
Biotium Live-or-Dye NucFix™ Red Staining Kit (200 assays)
Live-or-Dye NucFix™ Red is a chemically-reactive, cell membrane impermeable dye that specifically stains the nuclei of dead cells. The dye is able to enter into dead cells that have compromised membrane integrity and covalently label the cell nucleus, allowing for clear differentiation of live and dead cells by either microscopy or flow cytometry. Unlike other commonly used nuclear stains such as Propidium Iodide or DRAQ7™, NucFix™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye NucFix™ Red staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. The dye has an excitation/emission of 520/593 nm and is suitable for staining mammalian cells, yeast, or gram-negative bacteria. This kit contains sufficient reagent for 200 assays.
Biotium Live-or-Dye NucFix™ Red Staining Kit, Trial Size (50 assays)
Live-or-Dye NucFix™ Red is a chemically-reactive, cell membrane impermeable dye that specifically stains the nuclei of dead cells. The dye is able to enter into dead cells that have compromised membrane integrity and covalently label the cell nucleus, allowing for clear differentiation of live and dead cells by either microscopy or flow cytometry. Unlike other commonly used nuclear stains such as Propidium Iodide or DRAQ7™, NucFix™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye NucFix™ Red staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. The dye has an excitation/emission of 520/593 nm and is suitable for staining mammalian cells, yeast, or gram-negative bacteria. This trial size kit contains sufficient reagent for 50 assays.
Biotium Live-or-Dye™ 488/515 Fixable Viability Staining Kit (200 assays)
Live-or-Dye™ Fixable Viability Staining Kits are cell membrane impermeable amine-reactive dyes designed for discrimination between live and dead cells during flow cytometry. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. Live-or-Dye™ 488/515 is suitable for excitation by 488 nm laser line. This kit has sufficient reagent for 200 assays.