One-Step Reverse Transcription PCR Reagents
ABclonal Technology Lyophilization One Step RT-qPCR Probe Kit
The Lyophilization One-Step RT-qPCR Probe Kit is a ready-to-use, freeze-dried reagent for probe-based RT-qPCR, compatible with lyophilized powder or microspheres. It integrates ABScript III Reverse Transcriptase, hot-start Taq polymerase, heat-labile UDG, and RNase inhibitor for efficient one-tube reactions, boosting throughput. The dUTP/UDG system prevents contamination, with UDG degrading at 50°C to avoid interference. Stable at 45°C for 6 months, it contains <0.2% glycerol and lyoprotectants for long-term performance.
ABclonal Technology Lyophilization One Step RT-qPCR Probe Kit
The Lyophilization One-Step RT-qPCR Probe Kit is a ready-to-use, freeze-dried reagent for probe-based RT-qPCR, compatible with lyophilized powder or microspheres. It integrates ABScript III Reverse Transcriptase, hot-start Taq polymerase, heat-labile UDG, and RNase inhibitor for efficient one-tube reactions, boosting throughput. The dUTP/UDG system prevents contamination, with UDG degrading at 50°C to avoid interference. Stable at 45°C for 6 months, it contains <0.2% glycerol and lyoprotectants for long-term performance.
Cytek Biosciences Inc Classic++™ 1-Step RT-PCR Kit
Classic++™ 1-Step RT-PCR Kit ..... Cat. No. 31-5220-0050R ..... Size 50 rxns
LAMDA BIOTECH INC One-Step TaqProbe qRT-PCR Kit
A single-tube system designed for combined reverse transcription and probe-based real-time PCR analysis. Integrates RT and qPCR steps in a unified reaction for RNA quantification applications.
VWR International 95114-012 PerfeCTa® qPCR ToughMix®, Low ROX™ 1250 x 20 μL rxns (10 x 1.25 mL)
ToughMix additives neutralize PCR inhibitors to ensure reliable assay performance with a spectrum of starting materials including: clinical specimens, plants, soil, environmental or complex food matricesSuperior assay sensitivity and target precision with AccuStart™ II enzyme technology – maximum-yielding, engineered Taq DNA polymerase with stringent, ultrapure antibody hotstart
ABclonal Technology AFTSpin Multifunction DNA Purification Kit
The AFTSpin Multifunction DNA Purification Kit enables rapid gel recovery and PCR product purification using silica-column technology. DNA binds to the membrane in high-salt conditions, while impurities are removed via wash steps. A unique gel lysis/binding solution (yellow for easy pH monitoring) combines gel digestion and DNA binding, streamlining agarose recovery, PCR cleanup, and enzyme digestion purification. Delivers high-purity DNA with improved efficiency in a single kit.
ABclonal Technology AFTSpin Bacterial Fast RNA Extraction Kit
This kit can be used for rapid extraction of RNA from various bacterial samples, and does not rely on toxic reagents such as phenol and chloroform. The unique lysis buffer rapidly lyses cells from trace samples and inactivates cell RNA enzymes. The lysate mixture passes through a gDNA Remove Column, where gDNA is removed and RNA is filtered through. After adjusting the binding conditions by ethanol, RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt. In a series of rapid washing-centrifugation steps, the purified RNA is eluted from the silicon membrane with low salt RNase-free H2O. The extracted RNA can be directly used in RT-PCR, qPCR and RNA library constructing experiments.
ABclonal Technology AFTSpin EndoFree Plasmid Mini Kit
This kit uses a modified SDS-alkaline lysis method to lyse cells. Crude extract is selectively bound to the spin column by a unique endotoxin scavenger and centrifuged to remove endotoxin. The silicone matrix membrane in the spin column selectively binds plasmid DNA at high salt and low pH conditions, allowing for impurities and other bacterial components to be removed by Buffer PR and Buffer WB. The purified plasmid DNA is eluted from the silicone matrix membrane using low-salt and high-pH Buffer EB.