Isothermal Amplification Reagents
ABclonal Technology Pilot phi29 DNA Polymerase
Pilot phi29 DNA Polymerase differs from Phi29 DNA Polymerase by significantly enhancing protein thermal stability, reaction speed, and salt tolerance while retaining all the advantages of the latter. Pilot phi29 DNA Polymerase exhibits strong multiple strand displacement, continuous synthesis characteristics, and 3 -5 exonuclease activity.
GINKGO BIOWORKS INC (3P) 10 mL CFPS Economy kit. 1000 reactions of 10 uL. Contains lysate, reagent mix, and control plasmid.
Ginkgo’s cell free protein synthesis (CFPS) Economy kit is a beta version of our CFPS kit formulated to maximize protein expression per dollar spent. The E. coli transcription/translation system produces proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification.10 mL kit enables user to run 1000 reactions of 10 µL, 200 reactions of 50 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C.
Medchemexpress LLC Phi29 DNA polymerase | 125 U
Phi29 DNA polymerase is a protein-engineered Phi29 DNA polymerase expressed in E. coli, purified and isolated multiple times. It exhibits higher amplification efficiency and sensitivity compared to the wild-type enzyme, significantly shortening reaction times.
- High amplification efficiency and sensitivity: Greatly shortens reaction time.
- Special strand displacement activity: Possesses efficient continuous synthesis characteristics.
- Strong binding to templates: Can continuously synthesize DNA fragments up to 70kb without dissociating from the template.
- Strong 3′→5′ exonuclease proofreading function: Fidelity is 100 times higher than Taq DNA polymerase.
- High purity: Multiple column purifications ensure a clear single-purpose band in SDS-PAGE gel detection, with no E. coli DNA residue or endonuclease/exonuclease contamination in qPCR detection.
New England Biolabs, Inc. phi29-XT RCA DNA Ampification Kit - 500 reactions
The phi29-XT RCA DNA Amplification Kit includes everything needed for sensitive and robust rolling circle amplification (RCA), an isothermal amplification approach to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence. RCA enables several downstream applications, including DNA sequencing, cell-free protein expression, viral DNA enrichment, plasmid amplification, and padlock probe-based assays. The kit features phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability, sensitivity, and capable of generating higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any circular DNA sequence and dNTPs. Input material can be a purified plasmid, liquid media culture, a cultured colony from an agar plate, or a glycerol stock.
ABclonal Technology Pilot phi29 DNA Polymerase
Pilot phi29 DNA Polymerase differs from Phi29 DNA Polymerase by significantly enhancing protein thermal stability, reaction speed, and salt tolerance while retaining all the advantages of the latter. Pilot phi29 DNA Polymerase exhibits strong multiple strand displacement, continuous synthesis characteristics, and 3 -5 exonuclease activity.
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