Hygromycin B, also known as Hygrovetine, is an aminoglycoside antibiotic effective against both prokaryotic and eukaryotic cells. It functions by strongly inhibiting 70S and 80S ribosomes, thereby preventing protein synthesis through ribosomal translocation. Synthesized by *Streptomyces hygroscopicus*, it is widely used in research applications, including resistance selection in transgenic models.
- Active against prokaryotic and eukaryotic cells
- Inhibits protein synthesis by blocking 70S and 80S ribosomes
- Prevents ribosomal translocation
- Used in resistance selection experiments
- Applicable in cell proliferation/viability assays
- Suitable for Western blot and immunofluorescence analyses