Additional Cell Culture Reagents and Supplements
ILEX LIFE SCIENCES LLC 100X NEUROPAN-2 50 ML
PAN-Biotech Neuropan-2 Supplement (100x): Serum-Free Supplement for Cultivation of Neurons (50 ml). DESCRIPTION: Neuropan-2 is a supplement formulated to meet special requirements of neuronal cells. When supplemented with Neuropan Basal Medium, it allows long-term maintenance of normal phenotype and growth of neuronal cells, and maintains pure populations of neuronal cells without need for an astrocyte feeder. Neuropan-2 meets the special cell culture requirements of prenatal and embryonic neuronal cells from hippocampus, cortex, and other regions of the brain. APPLICATION: Neuropan-2 Supplement is a chemically-defined, serum-free supplement for the growth of primary embryonic neurons as well as embryonic neurospheres/CNS progenitor cells when combined with bFGF and EGF in Neuropan Basal Medium. COMPOSTION: Neuropan-2 Supplement contains insulin, transferrin, albumin, and hormones. LIQUID/POWDER: Liquid. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
STEMCELL Technologies Human Recombinant IL-7, ACF, Size: 1000 µg
Interleukin 7 (IL-7) is a member of the type I cytokine family that is critical for T and B cell development and survival. It is produced by non-hematopoietic cells in the thymus, lymphoid organs, and by bone marrow stromal cells (Lundström et al.). IL-7 binds to a receptor (IL-7R) composed of common gamma chain and IL-7Ra (CD127) and signals through the JAK/STAT and PI3K pathways. IL-7 regulates the survival of naïve and memory CD4+ and CD8+ T cells, γδ T cells, NK T cells, innate lymphoid cells, and regulatory T cells (Carrette & Surh). Although a deficiency in IL-7R still permits the generation of normal numbers of peripheral B cells in humans, stimulation of human B cell precursors with IL-7 could promote STAT5-dependent proliferation and survival in vitro (Clark et al.; Corfe & Paige). This product is animal component-free.
FUJIFILM BIOSCIENCES INC RPMI Medium 1640 - Liquid
RPMI Medium 1640 is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
Sigma Aldrich Fine Chemicals Biosciences IL 6 human Animal componen
IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains IL-6RÎ and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3. Murine IL-6 is inactive on human cells while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kDa protein containing 184 amino acid residues.
Promocell GmbH PromoCell Trypsin / EDTA 2 30 ml, 0,025 % Trypsin / 0,01 % EDTA
Trypsin is still the most widely employed enzyme used for cell detachment. PromoCell´s Trypsin Solutions exhibit standardized enzyme activity and contain trypsin from extra-pure lots.Trypsin / EDTA Solution having a ratio of 0.025% / 0.01%.
ILEX LIFE SCIENCES LLC 100X NEUROPAN-2 10 ML
PAN-Biotech Neuropan-2 Supplement (100x): Serum-Free Supplement for Cultivation of Neurons (10 ml). DESCRIPTION: Neuropan-2 is a supplement formulated to meet special requirements of neuronal cells. When supplemented with Neuropan Basal Medium, it allows long-term maintenance of normal phenotype and growth of neuronal cells, and maintains pure populations of neuronal cells without need for an astrocyte feeder. Neuropan-2 meets the special cell culture requirements of prenatal and embryonic neuronal cells from hippocampus, cortex, and other regions of the brain. APPLICATION: Neuropan-2 Supplement is a chemically-defined, serum-free supplement for the growth of primary embryonic neurons as well as embryonic neurospheres/CNS progenitor cells when combined with bFGF and EGF in Neuropan Basal Medium. COMPOSTION: Neuropan-2 Supplement contains insulin, transferrin, albumin, and hormones. LIQUID/POWDER: Liquid. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
STEMCELL Technologies Nicotinamide, Size: 100 g
Nicotinamide (NAM) is a cell culture supplement used in the differentiation of embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is an amide derivative of vitamin B3, a poly (ADP-ribose) polymerase (PARP) inhibitor, and represents the primary precursor of NAD+. Nicotinamide has also been shown to modulate stem cell differentiation in various applications, most notably for the differentiation of pancreatic mouse ES and iPS cells to pancreatic islet-like insulin-secreting cells.
Biolegend Recombinant Porcine IL-4 carri
Recombinant Porcine IL-4 (carrier-free); Reactivity: Pig; Apps: BA; Size: 100 μg
Cayman Chemical CYP2C9 InductIon ReporTR1ea
Early screening of drug candidate’s potential to induce or inhibit CYP2C9 is a critical step in the drug development process. The CYP2C9 Induction Reporter Assay Kit is a novel method to evaluate CYP2C9 induction by drug candidates. Cayman's CYP2C9 Induction Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing a Secreted Alkaline Phosphatase (SEAP) reporter regulated by the human CYP2C9 gene promoter. Cells grown on the CYP2C9 Reporter Reverse Transfection Strip Plate will introduce the reporter gene and express GRα as well as HNF4α. Test compounds that promote binding of the nuclear receptors to the CYP2C9 promoter initiates SEAP expression. SEAP activity is subsequently measured using a luminescence readout. The kit is simple to use and can be EAsily adapted to high-throughput screening for potential nuclear receptor-mediated CYP2C9 inducers.
Biolegend PE anti-mouse/human Bcl-6
PE anti-mouse/human Bcl-6 [IG191E/A8]; Isotype: Mouse IgG1; Reactivity: Human, Mouse; Apps: ICFC; Size: 25 tests
ATHENA ES Glucose Nutrient Mix
Glucose Nutrient Mix is an APF Certified™ medium supplement used for enhancing the expression of proteins when using Athena’s Hyper Broth™ or Glucose M9Y. The powder blend consists of glucose as a carbon source and is Glucose Nutrient Mix Powder 150g 0109. Free Available in dry-powder packets. supplemented with calcium chloride and magnesium sulfate. Glucose Nutrient Mix is included with both media, but can now be purchased separately in individual packets.
ATHENA ES Augmedium™, Powder 500
Augmedium™ is a medium additive which conditions cells prior to induction during a recombinant protein expression protocol. This pre-induction conditioner increases the level of chaperone proteins which can improve the fraction of product that accumulates as soluble protein. Augmedium™ is specifically intended for use with strains in which the target protein accumulates as an inclusion body or as an insoluble aggregate. Augmedium™ is supplied as a powder for preparation of 50x concentrated stock solutions. It is available in 100mL and 500mL amounts.
STEMCELL Technologies NeuroCult™ Differentiation Supplement (Mouse & Rat), Size: 50 mL
NeuroCult™ Differentiation Supplement (Mouse & Rat) is a standardized, serum-containing supplement for the differentiation of mouse and rat neural stem and progenitor cells into astrocytes, neurons, and oligodendrocytes when used in combination with NeuroCult™ Basal Medium (Mouse & Rat; Catalog #05700). This supplement is also a component of NeuroCult™ Differentiation Kit (Mouse & Rat; Catalog #05704).
ILEX LIFE SCIENCES LLC 50X NEUROPAN-27 100 ML
PAN-Biotech Neuropan-27 Supplement (50x): Defined Serum Substitute for Serum-Free Culture of CNS Neuronal Cells (100 ml). DESCRIPTION: Neuropan-27 is provided as a 50x concentrated serum substitute designed for the long-term growth and survival of hippocampal and other neuronal cells of the Central Nervous System (CNS). By supplementing Neuropan Basal Medium with Neuropan-27 and 0.5 mM L-Glutamine, excellent long-term viability of rat embryonic hippocampal neurons has been achieved after several weeks in culture. Glial cell growth is reduced to less than 0.1% for a largely pure neuronal cell culture. COMPOSTION: Neuropan-27 Supplement is a serum substitute of defined composition containing albumin, insulin, transferrin, lipids, and hormones. It is used as a supplement to Neuropan Basal Medium to support growth and survival of neuronal cells from the CNS. LIQUID/POWDER: Liquid. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
