Protein Extraction and Purification

Sartorius™ Vivaflow 200 Crossflow Cassettes Available on GSA/VA Contract for Federal Government customers only.

Offers a standard for ease of use, reliability and flexibility unrivalled by other filtration systems. Sartorius™ Vivaflow 200 Crossflow Cassettes are ready to use, reusable laboratory crossflow cassettes for concentrating up to 5L samples. Available in different MWCOs and membrane types for different application needs.

GE Healthcare GammaBind™ Plus Sepharose™ Medium Available on GSA/VA Contract for Federal Government customers only.

Engineered for better binding to rat and mouse IgG subclasses

Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose Available on GSA/VA Contract for Federal Government customers only.

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

GE Healthcare HiTrap™ Phenyl HP Columns Available on GSA/VA Contract for Federal Government customers only.

Prepacked columns for convenient and fast separations.  GE Healthcare Lifescience™ HiTrap™ Phenyl HP Columns are designed for use with a syringe, laboratory pump or chromatography system.

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads Available on GSA/VA Contract for Federal Government customers only.

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagent Available on GSA/VA Contract for Federal Government customers only.

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

Thermo Scientific™ HisPur™ Ni-NTA Resin Available on GSA/VA Contract for Federal Government customers only.

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets, EDTA-free

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.

Thermo Scientific™ Pierce™ Protein L Agarose

Affinity purify antibodies that have kappa light chains using Protein L offered in bottles, spin columns, filter plates and complete purification kits.

GE Healthcare Protein G Sepharose™ 4 Fast Flow Media Available on GSA/VA Contract for Federal Government customers only.

Rapid processing of large volumes, recoveries of 70 to 90%. GE Healthcare Protein G Sepharose™ 4 Fast Flow Media is recombinant protein G coupled to Sepharose 4 Fast Flow.

Thermo Scientific™ SulfoLink™ Coupling Resin

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

MilliporeSigma™ BioPak™ Disposable Ultrafilter Available on GSA/VA Contract for Federal Government customers only.

Nuclease-free, pyrogen-free water

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents Available on GSA/VA Contract for Federal Government customers only.

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

GE Healthcare HiTrap™ Heparin HP Columns Available on GSA/VA Contract for Federal Government customers only.

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. GE Healthcare Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Thermo Scientific™ UltraLink™ Hydrazide Resin

Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.

GE Healthcare HiTrap™ Protein G HP Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

Use for fast, easy separations of IgG from different species and subclasses. GE Healthcare Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.

Thermo Scientific™ CarboxyLink™ Immobilization Kit, 2 mL

Covalently immobilize peptide antigens and other carboxylate ligands for affinity purification using DADPA-activated agarose beads and EDC crosslinker.

Thermo Scientific™ Pierce™ His Protein Interaction Pull-Down Kit Available on GSA/VA Contract for Federal Government customers only.

Easily capture and affinity purify binding partners of His-tagged fusion proteins using nickel-chelate (Ni-IDA) agarose beads and this complete reagent set.

BD Recombinant Human β2 Microglobulin

Recombinant protein expressed in insect cells and purified by a combination of ion-exchange and gel-filtration methods

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

Invitrogen™ NanoOrange™ Protein Quantitation Kit, 200-2,000 assays Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution

Invitrogen™ MAGnify™ Chromatin Immunoprecipitation System Non-distribution product as customer accommodation.

Streamlined, optimized assay for the enrichment of chromatin/protein complexes and DNA recovery using magnetic bead capture technology.

Thermo Scientific™ Pierce™ Glutathione Agarose Available on GSA/VA Contract for Federal Government customers only.

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ HiPPR™ Detergent Removal Spin Columns, 0.1 mL

Efficiently remove detergents from samples with this high protein and peptide recovery detergent removal resin, especially for dilute protein samples.

Thermo Scientific™ ActivX™ TAMRA-FP Serine Hydrolase Probe

Tagged phosphonate probes to assay serine hydrolase active sites using fluorescence, biotin-affinity purification or detection, or mass spectrometry.

MilliporeSigma™ Pellicon™ XL Devices Available on GSA/VA Contract for Federal Government customers only.

Small-volume tangential flow filter device

Thermo Scientific™ Pierce™ Protein G Agarose Available on GSA/VA Contract for Federal Government customers only.

Affinity purify antibodies (IgG) with immobilized recombinant Protein G agarose offered in bottles, spin columns, filter plates and complete purification kits.

  spinner