Western Blotting Reagents, Membranes, Supplies
MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes
Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.
Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
MilliporeSigma™ Immobilon™-FL PVDF Transfer Membranes
Designed specifically for Western blots and protein sequencing. MilliporeSigma™ Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.
Thermo Scientific™ Pierce™ Fast Western Blot Kits, ECL
Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.
Invitrogen™ Invitrolon Novex Invitrolon PVDF/Filter Paper Sandwich
Pre-cut and pre-assembled and ready for protein blotting/transfer
Thermo Scientific™ Pierce™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Thermo Scientific™ SuperSignal™ West Dura Chemiluminescent Substrate
Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.
Thermo Scientific™ Pierce™ SuperSignal West Pico Fast Western Blot Kit
For picogram-level detection and Western blot development less than 1 hour after gel transfer.
Thermo Scientific™ PVDF Transfer Membrane, 0.2 µm, 26.5 cm x 3.75 m
Thermo Scientific Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for Western, Southern, and Northern blotting methods. This PVDF membrane has a 0.2-micron pore size and is ideal for colorimetric and chemiluminescent detection.
Invitrogen™ Novex™ Nitrocellulose Pre-Cut Blotting Membranes
Ideal for blotting proteins and nucleic acids
Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Invitrogen™ Novex™ PVDF Pre-cut Blotting Membranes
Pre-cut and pre-assembled—ready for protein blotting/transfer
Invitrogen™ Novex™ XCell™ II Blot Vertical Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes
GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls
Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Thermo Scientific™ Clean-Blot IP Detection Reagents and Kit
Avoid interference from denatured IP antibody fragments in Western blots of immunoprecipitated proteins with these HRP and AP conjugates.
GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent
Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.
Thermo Scientific™ HisProbe™-HRP Reagent and Kit
Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.
ZeptoMetrix SIV Western Blot Assay Kit
For the in vitro detection of antibodies to SIV in serum or plasma
GE Healthcare Amersham™ Hybond™ P 0.45μm PVDF Membrane
Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. GE Healthcare Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.
Invitrogen™ Novex™ WesternBreeze™ Chromogenic Kits
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate
Recommended HRP substrate for fluorescence imagers
GE Healthcare Whatman™ Grade GB003 Blotting Paper
Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.
Thermo Scientific™ Pierce™ NBT/BCIP Substrate Solutions
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Thermo Scientific™ Pierce™ SuperSignal West Dura Fast Western Blot Kits
For femtogram-level detection and Western blot development less than 1 hour after gel transfer.
GE Healthcare Amersham™ Hybond™ -N+ Membranes
Use for the highest sensitivity in DNA and RNA blotting. GE Healthcare Life Sciences™ Amersham™ Hybond™ -N+ Membrane is a positively charged nylon membranes for use with radioactive and nonradioactive chemiluminescence and chemifluorescence detection.
Invitrogen™ iBind™ Flex Western Device
Allows better antigen-antibody interaction and solutions flow between the iBind™ Card and membrane for maximum interaction between protein and antibody
Find what you need to support your Western blotting workflow, including nitrocellulose membranes, detection reagents, and more.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. After electrophoresis, the proteins in the gel are transferred to a membrane. The memabrane is then covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
The keys steps of western blotting include:
- Separate proteins by molecular weight using gel electrophoreses
- Protein gels, ladders, and stains
- Western blotting reagents and buffers
- Chamber systems
- Transfer the protein from the gel to a solid-support membrane
- Wet, semi-dry, and dry transfer systems
- Western blotting membranes
- Detect proteins visually using antibodies and substrates
- Antibodies and detection reagents
- Western blot buffers
- Imaging systems