Substrates for ELISA Detection

Thermo Scientific™ Pierce™ TMB Substrates

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ Pierce™ ABTS Substrates

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Thermo Scientific™ Pierce™ PNPP Substrates

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Thermo Scientific™ SuperSignal™ ELISA Femto Maximum Sensitivity Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Thermo Scientific™ Pierce™ Substrate Buffers for Soluble Substrates

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

Thermo Scientific™ SuperSignal™ ELISA Pico Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate

Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).

Thermo Scientific™ Pierce™ PNPP Substrates

An alkaline phosphatase substrate for ELISA applications

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Thermo Scientific™ Pierce™ OPD Substrates

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Thermo Scientific™ Pierce™ ONPG Substrate

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

BD TMB Substrate Reagent Set Available on GSA/VA Contract for Federal Government customers only.

For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3′, 5,5′ tetramethylbenzidine (TMB) in an organic solvent.