Substrates for ELISA Detection
Thermo Scientific™ Pierce™ TMB Substrates
Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific™ SuperSignal™ ELISA Pico Substrate
HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.
Thermo Scientific™ SuperSignal™ ELISA Femto Maximum Sensitivity Substrate
HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate
Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.
R&D Systems™ Substrate Reagent Pack
8 vials of Color Reagent A (stabilized hydrogen peroxide) and 8 vials of Color Reagent B (stabilized tetramethylbenzidine)
Thermo Scientific™ Pierce™ OPD Substrates
Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.
BD TMB Substrate Reagent Set
For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3′, 5,5′ tetramethylbenzidine (TMB) in an organic solvent.
Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate
Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).
Thermo Scientific™ Pierce™ PNPP Substrates
Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.
Thermo Scientific™ Pierce™ ABTS Substrates
Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.
Thermo Scientific™ Pierce™ ONPG Substrate
Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.
Thermo Scientific™ Pierce™ Substrate Buffers for Soluble Substrates
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.