Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
Rapidly separate cells or cell lysate from tissue debris after mechanical disruption using a 250-micron mesh filter and gravity or gentle centrifugation.
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Perform small-scale hydrophobic membrane-associated protein solubilization and enrichment with this simple reagent-based procedure.
Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents.
Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.