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Molecular Probes™ 7-AAD (7-Aminoactinomycin D)

Catalog No. A1310
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Quantity:
1 mg

7-aminoactinomycin D (7-AAD) is a fluorescent intercalator that undergoes a spectral shift upon association with DNA.

7-aminoactinomycin D (7-AAD) is a fluorescent intercalator that undergoes a spectral shift upon association with DNA. 7-AAD/DNA complexes can be excited by the 488 nm laser and has an emission maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy and flow cytometry. 7-AAD appears to be generally excluded from live cells, but can be used with cells that have been fixed and permeabilized. 7-AAD has been used for cell cycle analysis by flow cytometry. 7-AAD also binds selectively to GC regions of DNA yielding a distinct banding pattern in polytene chromosomes and chromatin for use in chromosome banding studies.

To make a concentrated stock solution from 1–2 mM, dissolve 7-AAD in either high quality anhydrous DMSO or DMF. Store frozen, desiccated and protected from light.

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Contains 1 vial of 7-AAD (1 mg).

Store in freezer (-5°C to -30°C) and protect from light.
Excitation Wavelength Range 488 nm
Dye Type Cell-Impermeant
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Quantity 1 mg
Detection Method Fluorescence
Emission 647 nm
Form Solid
Shipping Condition Room Temperature
Solubility DMSO (Dimethylsulfoxide)
Label Type Fluorescent Dye
Product Type Nuclear Labeling
SubCellular Localization Nucleic Acids, Nucleus
Molecular Weight (g/mol) 1270.45
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For Research Use Only. Not for use in diagnostic procedures.

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