ABO, Blood Group A Antigen Mouse anti-Human, Clone: HE-14, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247868B	Novus Biologicals NBP2478680.2MG	0.2 mg

Description

ABO, Blood Group A Antigen Monoclonal specifically detects ABO, Blood Group A Antigen in Human samples. It is validated for ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Agglutination.

Specifications

• Antigen: ABO, Blood Group A Antigen
• Concentration: 1.0 mg/mL
• Applications: Immunohistochemistry (Paraffin), Immunofluorescence, Agglutination
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Alias: A transferase, A3GALNT, A3GALT1, ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase), ABO glycosyltransferase, B transferase, B(A) alpha-1,3-galactosyltransferase, EC 2.4.1.37, EC 2.4.1.40, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, GTB, Histo-blood group A transferase, histo-blood group A2 transferase, histo-blood group ABO system transferase, Histo-blood group B transferase, NAGAT
• Gene Symbols: ABO
• Isotype: IgM κ
• Purification Method: Protein A or G purified
• Test Specificity: This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used.

• Clone: HE-14
• Dilution: Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, Agglutination
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 28
• Immunogen: Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.