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Promega AccessQuick™ RT-PCR System

Catalog No. PRA1702 Shop All Promega Corporation Products
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100 Reactions
20 Reactions
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Catalog No. No. of Reactions
PRA1702 100 Reactions
PR-A17-01 20 Reactions
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Catalog No. PRA1702 Supplier Promega Supplier No. A1702
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Description

Includes

AccessQuick Master Mix, 2X; AMV reverse transcriptase; Nuclease-free water

The AccessQuick RT-PCR System is an easy and convenient master mix for one-tube RT-PCR.

  • Convenient RT-PCR in One Tube
  • Mix contains Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer
  • Separate AMV RT provided for important no-RT control reactions
  • Simply combine AccessQuick™ Master Mix with AMV RT, RNA template, primers and water for efficient RT-PCR

For laboratory use.

Specifications

Concentration 2X (Master Mix)
Amplicon Size 323
Content And Storage • 2X Master Mix, 3 x 1 mL
• Reverse Transcriptase, 1 x 500 μL
• Nuclease-free Water, 2 x 1.25 mL

Store at –30° to –10°C.
Detection Method qPCR, RT-PCR
Form Liquid
Format Kit
PCR Method 1-step RT-PCR
Polymerase Tfl DNA Polymerase
Technique RT-PCR
DNase DNase-free
Host Cell E. coli
Includes Label or Dye No
Kit Type AccessQuick™ RT-PCR system
Label or Dye Unlabeled
No. of Tests 100 Tests
Optimal Reaction Temperature 68°C to 72°C
Primer Gene-Specific Primers
Product Line AccessQuick
Product Type RT-PCR Kit
Reverse Transcriptase AMV Reverse Transcriptase
Ribonuclease H Activity Yes
Sufficient For 100 Reactions
For Use With (Application) RT-PCR
Activation Time 2 min.
Compatibility RT-PCR
Fidelity (vs. Taq) 1X
Final Product Type cDNA
Hot Start No
No. of Reactions 100 Reactions
Overhang 3'-A
Reaction Format Separate Components
Reagent Type RT-PCR Reagent
Sample Type RNA
Starting Material RNA
Volume 50 μL
Source Tfl DNA Polymerase and AMV Reverse Transcriptase
Storage Buffer AMV/Tfl 5X Reaction Buffer
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