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Actin (Muscle Specific) Antibody (HHF35 + MSA/953) - Azide and BSA Free, Novus Biologicals™
SDP

Catalog No. NBP247663B Shop All R&D Systems Products
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NBP247663B 0.2 mg
NBP247663A 0.1 mg
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Catalog No. NBP247663B Supplier Novus Biologicals Supplier No. NBP2476630.2MG

Mouse Monoclonal Antibody

pan Actin Monoclonal antibody specifically detects Antigen in Human, Rabbit, Rat samples. It is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifications

Antigen pan Actin
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Classification Monoclonal
Clone HHF35 + MSA/953
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Formulation PBS with No Preservative
Gene Alias ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3
Gene Symbols ACTA1
Host Species Mouse
Immunogen Recombinant human actin fragment
Purification Method Protein A or G purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Stem Cell Markers
Primary or Secondary Primary
Gene ID (Entrez) 58
Test Specificity This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.AIt stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Target Species Human, Rat, Rabbit
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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