Ago2 Monoclonal Antibody (1B1-E2H5), Active Motif™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-2906	Active Motif 39854	10 μg
50-199-2905	Active Motif 39853	100 μg

Description

Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or 'Slicer' activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.

Specifications

• Antigen: Ago2
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
• Gene Alias: 1110029L17Rik; 2310051F07Rik; AGO1; AGO2; AI225898; AL022874; argonaute 2; argonaute 2, RISC catalytic component; Argonaute RISC catalytic component 2; argonaute RISC catalytic subunit 2; Argonaute1; Argonaute2; argonaute2 {ECO:0000255; AW546247; CTA-204B4.6; eIF2C; eIF2C 1; eIF-2C 1; eIF2C 2; eIF-2C 2; eIF2C 2 {ECO:0000255; eIF-2C 2 {ECO:0000255; EIF2C1; Eif2c2; ENSMUSG00000072493; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C 2 {ECO:0000255; eukaryotic translation initiation factor 2C, 2; GERp95; Gm10365; Golgi ER protein 95 kDa; hAgo1; hAgo2; HAMAP-Rule:MF_03031}; Kiaa4215; mAgo2; mKIAA4215; PAZ Piwi domain protein; Piwi/argonaute family protein meIF2C2; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer; Q10
• Gene Symbols: AGO2
• Isotype: IgG2a
• Purification Method: Protein G
• Product Type: Antibody

• Clone: 1B1-E2H5
• Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: Q9UKV8
• Gene ID (Entrez): 27161
• Immunogen: This Ago2 antibody was raised against a recombinant protein corresponding to amino acids 1-148 of human Ago2.
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: AGO2