Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Supplier:  Applied Biosystems™ 4387391

Catalog No. 43-873-91

Description

• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization. A Detection Enhancer is also provided as an optional reagent for amplification of templates with a high GC content or persistent secondary structure.

Specifications

• Content And Storage: Sufficient for 1000 reactions of 25 μL, contains:
  • 14 mL 2X RT-PCR Buffer
  • 1100 μL 25X RT-PCR Enzyme Mix
  • 25 mL Nuclease-free Water.
• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq Gold DNA Polymerase
• For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
• Product Line: AgPath-ID™, Ambion™
• Reverse Transcriptase: ArrayScript™
• Sufficient For: 1,000 Reactions
• Fidelity (vs. Taq): 25 X
• Sample Type: RNA

• Description: Works on all AB platforms, but is optimized for the 7500.
• Format: Tube
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Standard
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: One-Step RT-PCR Reagent
• Shipping Condition: Dry Ice
• Concentration: 25X
• No. of Reactions: 1000 Reactions
• Volume: 14 mL

For Veterinary Use Only.