AKT1, p Ser473 Antibody (17F6.B11), Biotin, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP221678

Catalog No. NBP221678

Description

AKT1 Monoclonal specifically detects AKT1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: AKT1 (p Ser473)
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Western Blot 1:500-1:3000, Flow Cytometry 1:10-1:1000, ELISA 1:20000, Immunohistochemistry 20 ug/ml, Immunocytochemistry/Immunofluorescence 1:500-1:3000, Immunohistochemistry-Paraffin 20 ug/ml
• Gene Alias: AKT, AKT serine/threonine kinase 1, AKT1m, CWS6, EC 2.7.11, EC 2.7.11.1, PKB, PKB alpha, PKBMGC99656, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, rac protein kinase alpha, RAC-ALPHA, RAC-alpha serine/threonine-protein kinas
• Host Species: Mouse
• Molecular Weight of Antigen: 55.7 kDa
• Quantity: 0.05 mg
• Research Discipline: Apoptosis, Autophagy, Cancer, Growth and Development, Hypoxia, mTOR Pathway, Neuronal Cell Markers, Phospho Specific, Signal Transduction
• Gene ID (Entrez): 207
• Reconstitution: Reconstitute with 100 ul deionized water (or equivalent)
• Content And Storage: Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 17F6.B11
• Conjugate: Biotin
• Gene Accession No.: P31749
• Gene Symbols: AKT1
• Immunogen: AKT1 [p Ser473] Antibody (17F6.B11) was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT11 protein, followed by hybridoma development. (Uniprot: P31749)
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
• Target Species: Human, Mouse, Rat, Monkey
• Form: Purified

For Research Use Only